Online citations, reference lists, and bibliographies.
← Back to Search

Sample Preparation And Assay Refinements For Pathogen Detection Platforms

D. Lim, E. Kearns, S. D. Leskinen, S. Magaña, Joyce M. Stroot, D. M. Hunter, Sarah M. Schlemmer
Published 2009 · Environmental Science, Engineering

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Food-borne and waterborne microbial pathogens are a potential problem in biowarfare and public health. Such pathogens can affect the health, combat readiness, and effectiveness of the warfighter in a battlefield environment and present potential threats to the civilian population through intentional or natural contamination of food and water. Conventional procedures to detect and identify microbial pathogens in food, water, and other materials can take days to perform and may provide inconclusive information. Research at the University of South Florida's Advanced Biosensors Laboratory (ABL) focuses on development of sample processing procedures and biosensor-based assays for rapid detection of biothreat agents. Rapid processing methods, including use of an automated concentrator of microorganisms in water, have been developed for complex matrix samples including ground beef, apple juice, produce, potable water and recreational water, enabling such samples to be directly tested by biosensor assays for target analytes. Bacillus atrophaeus spores and other bacteria can be concentrated from potable and recreational water at low levels with a dead-end hollow-fiber ultrafiltration concentration system. Target bacteria recovered by these processing procedures can be identified by evanescent wave, fiber optic biosensors or other detection platforms. Fiber optic biosensor assays have been improved to include subsequent PCR analysis and viability determination of captured target bacteria using broth enrichment and/or ATP luminescence.
This paper references
10.1023/A:1011125014586
PCR identification of Salmonella cells in food and stool samples after immunomagnetic separation
A. Španová (2000)
10.4315/0362-028X-62.7.711
Rapid detection of Escherichia coli O157:H7 in ground beef using a fiber-optic biosensor.
D. R. Demarco (1999)
10.1016/S0168-1605(98)00171-8
Rapid detection of stressed Salmonella spp. in dairy and egg products using immunomagnetic separation and PCR.
N. Rijpens (1999)
10.1139/W05-011
Comparison of 2 ultrafiltration systems for the concentration of seeded viruses from environmental waters.
J. Olszewski (2005)
10.1111/j.1365-2672.2006.02874.x
Portable continuous flow centrifugation and method 1623 for monitoring of waterborne protozoa from large volumes of various water matrices
U. Zuckerman (2006)
10.4315/0362-028X-65.4.596
Detection of Escherichia coli O157:H7 in 10- and 25-gram ground beef samples with an evanescent-wave biosensor with silica and polystyrene waveguides.
D. R. Demarco (2002)
10.1039/B110990C
Microsample preparation by dielectrophoresis: isolation of malaria.
P. Gascoyne (2002)
10.1128/JCM.34.3.516-519.1996
Isolation of enterohemorrhagic Escherichia coli O157 strains from patients with hemolytic-uremic syndrome by using immunomagnetic separation, DNA-based methods, and direct culture.
H. Karch (1996)
10.1128/JCM.42.3.1042-1047.2004
Rapid Quantification of Yersinia enterocolitica in Pork Samples by a Novel Sample Preparation Method, Flotation, Prior to Real-Time PCR
P. Wolffs (2004)
10.1016/S0167-7012(03)00133-7
Confirmation of viable E. coli O157:H7 by enrichment and PCR after rapid biosensor detection.
T. B. Tims (2003)
10.1111/J.1745-4581.2001.TB00250.X
DIRECT DETECTION OF ESCHERICHIA COLI 0157:H7 IN UNPASTEURIZED APPLE JUICE WITH AN EVANESCENT WAVE BIOSENSOR
D. R. Demarco (2001)
10.1128/JCM.33.2.336-341.1995
Detection of Yersinia pestis fraction 1 antigen with a fiber optic biosensor.
L. Cao (1995)
A buyer's guide to DNA and RNA prep kits
J. Chua (2004)
10.1046/j.1365-2672.2002.01570.x
Concentration of Cryptosporidium, microsporidia and other water‐borne pathogens by continuous separation channel centrifugation
M. Borchardt (2002)
10.1016/J.JCV.2003.10.002
Assessment of automated DNA extraction coupled with real-time PCR for measuring Epstein-Barr virus load in whole blood, peripheral mononuclear cells and plasma.
S. Fafi-Kremer (2004)
10.1128/CMR.18.4.583-607.2005
Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare
D. Lim (2005)
10.1046/j.1472-765X.1997.00069.x
A rapid sample preparation method for PCR detection of food pathogens based on buoyant density centrifugation
R. Lindqvist (1997)
10.1128/AEM.71.11.6878-6884.2005
Development of a Rapid Method for Simultaneous Recovery of Diverse Microbes in Drinking Water by Ultrafiltration with Sodium Polyphosphate and Surfactants
V. Hill (2005)
10.1111/J.1745-4581.2002.TB00015.X
RECOVERY OF ESCHERICHIA COLI O157:H7 FROM FIBER OPTIC WAVEGUIDES USED FOR RAPID BIOSENSOR DETECTION
M. F. Kramer (2002)
10.1016/0956-5663(93)80012-E
A fiber optic biosensor: combination tapered fibers designed for improved signal acquisition
G. M. Anderson (1993)
10.4315/0362-028X-64.11.1744
Combination of immunomagnetic separation and polymerase chain reaction for the simultaneous detection of Listeria monocytogenes and Salmonella spp. in food samples.
H. Hsih (2001)
10.1093/JAOAC/81.2.403
Direct 24-hour presumptive enumeration of Escherichia coli O157:H7 in foods using hydrophobic grid membrane filter followed by serological confirmation: collaborative study.
P. Entis (1998)
10.1111/j.1365-2672.2008.03757.x
Automated concentration and recovery of micro‐organisms from drinking water using dead‐end ultrafiltration
E. Kearns (2008)
10.1016/0003-2697(92)90440-I
Detection of Clostridium botulinum toxin A using a fiber optic-based biosensor.
R. A. Ogert (1992)
10.4315/0362-028X-67.5.1036
Detection of Bacillus spores using PCR and FTA filters.
K. Lampel (2004)
10.1016/0924-2244(91)90734-Z
Methods for the separation and concentration of bacteria from foods
M. J. Payne (1991)
10.4315/0362-028X-67.1.46
A rapid and automated fiber optic-based biosensor assay for the detection of Salmonella in spent irrigation water used in the sprouting of sprout seeds.
M. F. Kramer (2004)
10.1128/AEM.00052-08
Rapid Ultrafiltration Concentration and Biosensor Detection of Enterococci from Large Volumes of Florida Recreational Water
S. D. Leskinen (2008)
10.1117/1.601146
Portable multichannel fiber optic biosensor for field detection
J. Golden (1997)
10.1002/ELPS.200405899
Insulator‐based dielectrophoresis for the selective concentration and separation of live bacteria in water
B. Lapizco-Encinas (2004)
10.1016/J.BIOS.2005.02.012
Rapid PCR confirmation of E. coli O157:H7 after evanescent wave fiber optic biosensor detection.
Joyce M. Simpson (2005)
10.1186/1471-2334-4-15
Fast detection of Noroviruses using a real-time PCR assay and automated sample preparation
M. Schmid (2004)
10.1016/S0168-1605(97)00054-8
Preparation of PCR samples from food by a rapid and simple centrifugation technique evaluated by detection of Escherichia coli O157:H7.
R. Lindqvist (1997)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar