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Taking Cell-Matrix Adhesions To The Third Dimension
E. Cukierman, R. Pankov, D. R. Stevens, K. Yamada
Published 2001 · Biology, Medicine
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Adhesions between fibroblastic cells and extracellular matrix have been studied extensively in vitro, but little is known about their in vivo counterparts. Here, we characterized the composition and function of adhesions in three-dimensional (3D) matrices derived from tissues or cell culture. “3D-matrix adhesions” differ from focal and fibrillar adhesions characterized on 2D substrates in their content of α5β1 and αvβ3 integrins, paxillin, other cytoskeletal components, and tyrosine phosphorylation of focal adhesion kinase (FAK). Relative to 2D substrates, 3D-matrix interactions also display enhanced cell biological activities and narrowed integrin usage. These distinctive in vivo 3D-matrix adhesions differ in structure, localization, and function from classically described in vitro adhesions, and as such they may be more biologically relevant to living organisms.
This paper references
COLLAGEN SUBSTRATA FOR STUDIES ON CELL BEHAVIOR
Tom Elsdale (1972)
Dynamic reciprocity revisited: a continuous, bidirectional flow of information between cells and the extracellular matrix regulates mammary epithelial cell function.
C. Roskelley (1995)
Integrins: emerging paradigms of signal transduction.
M. Schwartz (1995)
Integrins: Versatility, modulation, and signaling in cell adhesion
R. Hynes (1992)
Molecules in focus Paxillin
Christopher E. Turner (1998)
Guidance of myogenic cell migration by oriented deposits of fibronectin.
D. Turner (1983)
Focal Adhesion Kinase: a regulator of focal adhesion dynamics and cell movement
J. Parsons (2000)
Cell-matrix contact structures
J. Adams (2001)
Focal adhesions, contractility, and signaling.
K. Burridge (1996)
Single subunit chimeric integrins as mimics and inhibitors of endogenous integrin functions in receptor localization, cell spreading and migration, and matrix assembly
S. LaFlamme (1994)
Focal adhesions: a nexus for intracellular signaling and cytoskeletal dynamics.
S. K. Sastry (2000)
Molecular interactions in cell adhesion complexes.
K. Yamada (1997)
The structural and mechanical complexity of cell-growth control
S. Huang (1999)
The biology of cell locomotion within three-dimensional extracellular matrix
P. Friedl (2000)
Synergistic roles for receptor occupancy and aggregation in integrin transmembrane function
S. Miyamoto (1995)
Immunofluorescence detection of ezrin/radixin/moesin (ERM) proteins with their carboxyl-terminal threonine phosphorylated in cultured cells and tissues.
K. Hayashi (1999)
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P. Heikkilä (2005)
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M. Bissell (2003)
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S. Filippov (2005)
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P. D. Cerdán (2003)
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Fan He (2013)
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A. Yamashita (2009)
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B. Weigelt (2009)
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Organotypic Cultures of Hepg2 Cells for In Vitro Toxicity Studies
D. Müller (2011)
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M. Kirsch (2019)
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R. Zaidel-Bar (2007)
Proliferation and adhesion capability of human gingival fibroblasts onto zirconia, lithium disilicate and feldspathic veneering ceramic in vitro.
S. Teté (2014)
High Cell Density Three-Dimensional Neural Co-Cultures Require Continuous Medium Perfusion for Survival
D. Cullen (2006)
h2-calponin Is Regulated by Mechanical Tension and Modifies the Function of Actin Cytoskeleton*
M. Hossain (2005)
Reorganization of actin filaments enhances chondrogenic differentiation of cells derived from murine embryonic stem cells.
Z. Zhang (2006)
A mathematical model of glioblastoma tumor spheroid invasion in a three-dimensional in vitro experiment.
A. M. Stein (2007)
Defined substrates for human embryonic stem cell growth identified from surface arrays.
Ratmir Derda (2007)
Tissue engineering: a new frontier in physiological genomics.
M. C. Petersen (2007)
Collagen IV regulates Caco-2 cell spreading and p130Cas phosphorylation by FAK-dependent and FAK-independent pathways
M. Sanders (2008)
Ovarian normal and tumor-associated fibroblasts retain in vivo stromal characteristics in a 3-D matrix-dependent manner.
R. Quiros (2008)
Stem cells, microenvironment mechanics, and growth factor activation.
Rebeca M. Tenney (2009)
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Mira Töyli (2011)
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Changge Fang (2013)
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