← Back to Search
Nanoparticle-Based Bio-Bar Codes For The Ultrasensitive Detection Of Proteins
J. Nam, C. S. Thaxton, C. Mirkin
Published 2003 · Medicine, Chemistry
Download PDFAnalyze on Scholarcy
An ultrasensitive method for detecting protein analytes has been developed. The system relies on magnetic microparticle probes with antibodies that specifically bind a target of interest [prostate-specific antigen (PSA) in this case] and nanoparticle probes that are encoded with DNA that is unique to the protein target of interest and antibodies that can sandwich the target captured by the microparticle probes. Magnetic separation of the complexed probes and target followed by dehybridization of the oligonucleotides on the nanoparticle probe surface allows the determination of the presence of the target protein by identifying the oligonucleotide sequence released from the nanoparticle probe. Because the nanoparticle probe carries with it a large number of oligonucleotides per protein binding event, there is substantial amplification and PSA can be detected at 30 attomolar concentration. Alternatively, a polymerase chain reaction on the oligonucleotide bar codes can boost the sensitivity to 3 attomolar. Comparable clinically accepted conventional assays for detecting the same target have sensitivity limits of ∼3 picomdar, six orders of magnitude less sensitive than what is observed with this method.
This paper references
Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.
R. Saiki (1985)
Nanoparticles with Raman spectroscopic fingerprints for DNA and RNA detection.
Y. Cao (2002)
Self-assembly of DNA-streptavidin nanostructures and their use as reagents in immuno-PCR.
C. Niemeyer (1999)
Printing proteins as microarrays for high-throughput function determination.
G. MacBeath (2000)
A fluorescence-based method for determining the surface coverage and hybridization efficiency of thiol-capped oligonucleotides bound to gold thin films and nanoparticles.
L. Demers (2000)
Scanometric DNA array detection with nanoparticle probes.
T. Taton (2000)
Ultrasensitive assay of prostate-specific antigen used for early detection of prostate cancer relapse and estimation of tumor-doubling time after radical prostatectomy.
H. Yu (1995)
A DNA-based method for rationally assembling nanoparticles into macroscopic materials
C. Mirkin (1996)
Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems.
S. Bustin (2002)
Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates.
T. Sano (1992)
Polymerase chain reaction techniques.
R. Gibbs (1991)
Universal immuno-PCR for ultra-sensitive target protein detection.
H. Zhou (1993)
The developments of semisynthetic DNA-protein conjugates.
C. Niemeyer (2002)
High sensitivity multianalyte immunoassay using covalent DNA-labeled antibodies and polymerase chain reaction.
E. R. Hendrickson (1995)
A quantitative immuno-PCR assay for the detection of mumps-specific IgG.
A. McKie (2002)
Ultrasensitive detection of prostate-specific antigen by a time-resolved immunofluorometric assay and the Immulite immunochemiluminescent third-generation assay: potential applications in prostate and breast cancers.
R. Ferguson (1996)
This paper is referenced by
Nanoparticles and their biological and environmental applications.
W. Liu (2006)
Detection of respiratory syncytial virus using nanoparticle amplified immuno-polymerase chain reaction.
J. W. Perez (2011)
Fluorescence turn-on sensing of lectins with mannose-substituted tetraphenylethenes based on aggregation-induced emission.
T. Sanji (2010)
Two‐step signal amplification for high‐sensitivity detection of biomarkers using gold nanoparticle–based conjugates
P. Zhang (2019)
Construction and characterization of Cu²⁺, Ni²⁺, Zn²⁺, and Co²⁺ modified-DNA crystals.
S. R. Dugasani (2015)
Magneto-controlled flow-injection device for electrochemical immunoassay of alpha-fetoprotein on magnetic beads using redox-active ferrocene derivative polymer nanospheres.
B. Li (2019)
Graphene-based nanomaterials in biosystems
Na Lu (2018)
Role of Nanocomposites and Nanostructured Biomaterials in Biomedical and Nanobiotechnology.
A. Ahmed (2013)
On the structure of nanorods and nanowires with pentagonal cross-sections
J. Reyes-Gasga (2006)
MCM-41 mesoporous material modified carbon paste electrode for the determination of cardiac troponin I by anodic stripping voltammetry.
Huishi Guo (2005)
Biomarkers in Cancer Staging, Prognosis and Treatment Selection
J. Ludwig (2005)
Structural and fluorescence quenching characterization of hematite nanoparticles.
Ahmed S. Al-Kady (2011)
Selective aggregation of PAMAM dendrimer nanocarriers and PAMAM/ZnPc nanodrugs on human atheromatous carotid tissues: a photodynamic therapy for atherosclerosis
Nikolaos Spyropoulos-Antonakakis (2015)
Ultra-sensitive suspended graphene nanocomposite cancer sensors with strong suppression of electrical noise.
B. Zhang (2012)
NUCLEC ACDS AND OTHER ANALYTES
Proteins conjugation with ZnO sol–gel nanopowders
L. Armelao (2011)
Magnetic nanoparticle-enhanced biosensor based on grating-coupled surface plasmon resonance.
Yi Wang (2011)
Fundamentals and application of ordered molecular assemblies to affinity biosensing.
Zimple Matharu (2012)
A novel permanganate-sensitive fluorescent nano-chemosensor assembled with a new 8-hydroxyquinoline-functionalized SBA-15.
Mohammad Reza Ganjali (2012)
Role of nanotechnology in the diagnosis and treatment of alzheimer's disease
Kristen M. Jaruszewski (2012)
CdS nanoparticles functionalized colloidal carbon particles: preparation, characterization and application for electrochemical detection of thrombin.
Xiao-Ya Dong (2011)
Factors affecting T cell responses induced by fully synthetic glyco-gold-nanoparticles.
S. Fallarini (2013)
Rapid Synthesis of Ag Nanoparticles Using Henna Extract for the Fabrication of Photoabsorption Enhanced Dye Sensitized Solar Cell (PE-DSSC)
S. C. G. kiruba Daniel (2013)
2. Molecular Recognition
Wolfgang Pompe (2013)
Laser-induced synthesis of silver nanoparticles and their conjugation with protein
Deepti Joshi (2014)
Gold Nanoparticles as (Bio)Chemical Sensors
Miguel Peixoto de Almeida (2014)
Deriving the colloidal synthesis of crystalline nanosheets to create self-assembly monolayers of nanoclusters.
Yi Liu (2014)
Cancer biomarker detection: recent achievements and challenges.
L. Wu (2015)
A methanol VOC sensor using divalent metal ion-modified 2D DNA lattices
Hyeong-U Kim (2015)
Carbofuran bio-barcode immunoassay kit and application thereof
Dynamic light scattering (DLS)-based immunoassay for ultra-sensitive detection of tumor marker protein.
C. Li (2016)
A density functional study on the electronic structure, nature of bonding and reactivity of NO adsorbing Rh0/±n (n = 2–8) clusters
A. Dutta (2018)See more