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Differential Display Of Eukaryotic Messenger RNA By Means Of The Polymerase Chain Reaction.

P. Liang, A. Pardee
Published 1992 · Biology, Medicine

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Effective methods are needed to identify and isolate those genes that are differentially expressed in various cells or under altered conditions. This report describes a method to separate and clone individual messenger RNAs (mRNAs) by means of the polymerase chain reaction. The key element is to use a set of oligonucleotide primers, one being anchored to the polyadenylate tail of a subset of mRNAs, the other being short and arbitrary in sequence so that it anneals at different positions relative to the first primer. The mRNA subpopulations defined by these primer pairs were amplified after reverse transcription and resolved on a DNA sequencing gel. When multiple primer sets were used, reproducible patterns of amplified complementary DNA fragments were obtained that showed strong dependence on sequence specificity of either primer.
This paper references
Efficient double stranded sequencing of cDNA clones containing long poly(A) tails using anchored poly(dT) primers.
A. S. Khan (1991)
G1 events and regulation of cell proliferation.
A. Pardee (1989)
Regulation of inducible and tissue-specific gene expression.
T. Maniatis (1987)
DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.
J. Williams (1990)
Synchronization of tumor and normal cells from G1 to multiple cell cycles by lovastatin.
K. Keyomarsi (1991)
Molecular cloning and expression of the human deoxythymidylate kinase gene in yeast.
J. Su (1991)
Cell cycle regulated synthesis of stable mouse thymidine kinase mRNA is mediated by a sequence within the cDNA.
R. Hofbauer (1987)
Tumor progression in four mammary epithelial cell lines derived from the same patient.
V. Band (1990)
Do the poly(A) tail and 3′ untranslated region control mRNA translation?
R. Jackson (1990)
Sequence identification of 2,375 human brain genes
M. Adams (1992)
Positive selection of candidate tumor-suppressor genes by subtractive hybridization.
S. Lee (1991)
High resolution two-dimensional electrophoresis of proteins.
P. O'Farrell (1975)
Driving the cell cycle: M phase kinase, its partners, and substrates
B. Lewin (1990)
Cloning of cDNA sequences of hormone-regulated genes from the MCF-7 human breast cancer cell line.
P. Masiakowski (1982)

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