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Sequence And Expression Of MRNAs Encoding The α1 And α2 Subunits Of A DHP-sensitive Calcium Channel
S. Ellis, M. E. Williams, N. R. Ways, R. Brenner, A. Sharp, A. T. Leung, K. Campbell, E. Mckenna, W. J. Koch, A. Hui, A. Schwartz, M. Harpold
Published 1988 · Biology
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Complementary DNAs were isolated and used to deduce the primary structures of the alpha 1 and alpha 2 subunits of the dihydropyridine-sensitive, voltage-dependent calcium channel from rabbit skeletal muscle. The alpha 1 subunit, which contains putative binding sites for calcium antagonists, is a hydrophobic protein with a sequence that is consistent with multiple transmembrane domains and shows structural and sequence homology with other voltage-dependent ion channels. In contrast, the alpha 2 subunit is a hydrophilic protein without homology to other known protein sequences. Nucleic acid hybridization studies suggest that the alpha 1 and alpha 2 subunit mRNAs are expressed differentially in a tissue-specific manner and that there is a family of genes encoding additional calcium channel subtypes.
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