Online citations, reference lists, and bibliographies.
← Back to Search

High-resolution Molecular Discrimination By RNA.

R. Jenison, S. Gill, A. Pardi, B. Polisky
Published 1994 · Chemistry, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
Species of RNA that bind with high affinity and specificity to the bronchodilator theophylline were identified by selection from an oligonucleotide library. One RNA molecule binds to theophylline with a dissociation constant Kd of 0.1 microM. This binding affinity is 10,000-fold greater than the RNA molecule's affinity for caffeine, which differs from theophylline only by a methyl group at nitrogen atom N-7. Analysis by nuclear magnetic resonance indicates that this RNA molecule undergoes a significant change in its conformation or dynamics upon theophylline binding. Binding studies of compounds chemically related to theophylline have revealed structural features required for the observed binding specificity. These results demonstrate the ability of RNA molecules to exhibit an extremely high degree of ligand recognition and discrimination.
This paper references
10.1093/CLINCHEM/27.11.1931
Theophylline determination by "high-pressure" liquid chromatography.
L. Broussard (1981)
10.1038/345836A0
Formation of a stable triplex from a single DNA strand.
V. Sklenár̆ (1990)
10.1038/355564A0
Selection of single-stranded DNA molecules that bind and inhibit human thrombin
L. C. Bock (1992)
10.1038/355850A0
Selection in vitro of single-stranded DNA molecules that fold into specific ligand-binding structures
A. Ellington (1992)
10.1016/0022-2836(92)90870-P
Selection of high affinity RNA ligands to the bacteriophage R17 coat protein.
D. Schneider (1992)
10.1038/346818A0
In vitro selection of RNA molecules that bind specific ligands
A. Ellington (1990)
10.1073/PNAS.90.23.11227
High-affinity RNA ligands to basic fibroblast growth factor inhibit receptor binding.
D. Jellinek (1993)
10.1017/S0033583500003875
RNA structure and NMR spectroscopy.
G. Varani (1991)
10.1073/PNAS.89.15.6988
RNA pseudoknots that inhibit human immunodeficiency virus type 1 reverse transcriptase.
C. Tuerk (1992)
10.1093/CLINCHEM/27.9.1575
Fluorescence polarization immunoassay. iii. an automated system for therapeutic drug determination.
M. Jolley (1981)
10.1111/J.1432-1033.1988.TB14425.X
Nucleic acids and nuclear magnetic resonance.
F. van de Ven (1988)
10.1021/BI00072A002
Three small ribooligonucleotides with specific arginine sites.
G. Connell (1993)
10.1021/JA00036A065
Stereospecific recognition of tryptophan agarose by in vitro selected RNA
M. Famulok (1992)
10.1126/SCIENCE.2200121
Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase.
C. Tuerk (1990)
10.1016/S0196-0644(86)80009-9
Rapid assay of serum theophylline levels.
T. Reinecke (1986)
10.1038/364550A0
An RNA motif that binds ATP
M. Sassanfar (1993)
10.1016/0003-2697(83)90052-0
A simple method for the estimation of amino groups on insoluble matrix beads.
G. Antoni (1983)
10.1080/01971529008053259
Immunoassay of theophylline by latex particle counting.
S. Poncelet (1990)
10.1016/0022-2836(91)90015-X
Escherichia coli sigma 70 and NusA proteins. I. Binding interactions with core RNA polymerase in solution and within the transcription complex.
S. Gill (1991)
10.1016/0092-8674(91)90527-6
HIV-1 rev regulation involves recognition of non-Watson-Crick base pairs in viral RNA
D. Bartel (1991)



This paper is referenced by
10.1016/j.bios.2012.07.064
A new strategy based on aptasensor to time-resolved fluorescence assay for adenosine deaminase activity.
K. Zhang (2013)
10.1080/15257779508010694
Efficient Synthesis of 2′-Amino-2′-deoxypyrimidine 5′-Triphosphates
D. Mcgee (1995)
10.1038/NBT0896-1021
An enzyme-linked oligonucleotide assay
D. W. Drolet (1996)
10.1089/OLI.1.1998.8.265
Oligonucleotide inhibitors of P-selectin-dependent neutrophil-platelet adhesion.
R. Jenison (1998)
10.1261/RNA.576908
A simple ligation-based method to increase the information density in sequencing reactions used to deconvolute nucleic acid selections.
Jessica L. Childs-Disney (2008)
10.1088/0957-4484/19/41/415605
Fluorescent detection of ATP based on signaling DNA aptamer attached silica nanoparticles.
Y. Wang (2008)
10.1039/b814993c
Selection and elution of aptamers using nanoporous sol-gel arrays with integrated microheaters.
Seung-Min Park (2009)
10.1021/ja1092002
Measuring single small molecule binding via rupture forces of a split aptamer.
Thi-Huong Nguyen (2011)
10.1093/NAR/GKH835
Selection and evolution of NTP-specific aptamers.
Laure Weill (2004)
10.1002/BIP.10519
Catalytic roles for proton transfer and protonation in ribozymes.
P. Bevilacqua (2004)
Kinetic discriminations for selective analysis in complex mixtures : applications in imaging
Jérôme Querard (2015)
10.22215/etd/2018-12721
Augmenting the Detection of Verotoxin-Producing Escherichia coli Through use of Fluorescently-labeled DNA Oligonucleotides
Dylan Jones Fox-Altherr (2018)
10.3390/s140203737
DNA-Aptamers Binding Aminoglycoside Antibiotics
N. Nikolaus (2014)
10.1016/j.bpj.2014.11.001
Tetracycline determines the conformation of its aptamer at physiological magnesium concentrations.
A. J. Reuss (2014)
10.1261/rna.1346609
Leakage and slow allostery limit performance of single drug-sensing aptazyme molecules based on the hammerhead ribozyme.
Chamaree de Silva (2009)
10.1016/j.bios.2013.07.037
Colorimetric detection of controlled assembly and disassembly of aptamers on unmodified gold nanoparticles
S. C. Gopinath (2014)
10.1016/j.ab.2019.05.012
Comparison of different label-free imaging high-throughput biosensing systems for aptamer binding measurements using thrombin aptamers.
C. Rath (2019)
10.1080/00032719.2014.924009
Determination of Enrofloxacin in Bovine Milk by a Novel Single-Stranded DNA Aptamer Chemiluminescent Enzyme Immunoassay
Hengjia Ni (2014)
10.1016/S0076-6879(96)67021-9
Affinity selection-amplification from randomized ribooligonucleotide pools.
J. Ciesiołka (1996)
10.1016/j.bios.2014.02.004
Small molecule detection in solution via the size contraction response of aptamer functionalized nanoparticles.
Omar A. Alsager (2014)
10.1093/NAR/29.7.1631
Cooperative binding of effectors by an allosteric ribozyme.
A. Jose (2001)
10.1016/B978-0-12-396983-5.00018-1
Nucleic Acid Aptamers for Biomaterials Development
Mark R. Battig (2014)
10.1016/B978-0-12-801122-5.00019-2
Facile characterization of aptamer kinetic and equilibrium binding properties using surface plasmon resonance.
Andrew L. Chang (2014)
10.1016/j.omtn.2018.09.013
Phosphorothioate-Modified AP613-1 Specifically Targets GPC3 when Used for Hepatocellular Carcinoma Cell Imaging
Lili Dong (2018)
10.1016/j.actbio.2013.06.016
Aptamers that bind to the hemagglutinin of the recent pandemic influenza virus H1N1 and efficiently inhibit agglutination.
S. C. Gopinath (2013)
10.1007/978-1-4419-8252-0_3
Single Molecule Detection with an Aptamer-Integrated Nanopore
L. Gu (2011)
10.1007/BF01718707
Screening chemical libraries for nucleic-acid-binding drugs by in vitro selection: A test case with lividomycin
S. Lato (2005)
10.1002/(SICI)1099-1352(199607)9:4<275::AID-JMR337>3.0.CO;2-8
Three‐dimensional model of a selective theophylline‐binding RNA molecule
C. Tung (1996)
10.1016/S0167-2789(97)00104-8
Landscapes and molecular evolution
P. Schuster (1997)
10.1038/41987
Torsional oscillations and the magnetic field within the Earth's core
S. Zatman (1997)
10.1016/B978-012233210-4/50012-8
11 – Progress towards Therapeutic Application of SELEX-derived Aptamers
B. Polisky (1998)
10.1016/S0969-2126(99)80102-6
Design of allosteric hammerhead ribozymes activated by ligand-induced structure stabilization.
G. Soukup (1999)
See more
Semantic Scholar Logo Some data provided by SemanticScholar