← Back to Search
Suppression Of ICE And Apoptosis In Mammary Epithelial Cells By Extracellular Matrix
N. Boudreau, C. J. Sympson, Z. Werb, M. Bissell
Published 1995 · Biology, Political Science, Medicine
Download PDFAnalyze on Scholarcy
Apoptosis (programmed cell death) plays a major role in development and tissue regeneration. Basement membrane extracellular matrix (ECM), but not fibronectin or collagen, was shown to suppress apoptosis of mammary epithelial cells in tissue culture and in vivo. Apoptosis was induced by antibodies to beta 1 integrins or by overexpression of stromelysin-1, which degrades ECM. Expression of interleukin-1 beta converting enzyme (ICE) correlated with the loss of ECM, and inhibitors of ICE activity prevented apoptosis. These results suggest that ECM regulates apoptosis in mammary epithelial cells through an integrin-dependent negative regulation of ICE expression.
This paper references
Epidermal growth factor or transforming growth factor alpha is required for kidney tubulogenesis in matrigel cultures in serum-free medium.
M. Taub (1990)
C. elegans cell survival gene ced-9 encodes a functional homolog of the mammalian proto-oncogene bcl-2
M. Hengartner (1994)
The C. elegans cell death gene ced-3 encodes a protein similar to mammalian interleukin-1β-converting enzyme
J. Yuan (1993)
Viral inhibition of inflammation: Cowpox virus encodes an inhibitor of the interleukin-1β converting enzyme
C. Ray (1992)
the faint ethidium bromide staining observed in DNA extracted from cells cultured on EHS is attributable to randomly degraded DNA in the initial EHS preparations
M Taub (1990)
was added at a dilution of 1:200 for 2 days to MECs that had been cultured for 2 days on filters
C H Damsky (1982)
Proteinases of the mammary gland: developmental regulation in vivo and vectorial secretion in culture.
R. Talhouk (1991)
Structure-function relationships in the collagenase family member transin.
R. Sánchez-López (1988)
New mammary epithelial and fibroblastic cell clones in coculture form structures competent to differentiate functionally
E. Reichmann (1989)
Induction of apoptosis in fibroblasts by IL-1β-converting enzyme, a mammalian homolog of the C. elegans cell death gene ced-3
M. Miura (1993)
The extracellular matrix as a cell survival factor.
J. Meredith (1993)
CID-9 cells were cultured on Milli-Cell CM filters (Millipore) under conditions that promote synthesis and vectorial secretion of milk proteins
were cultured in Dulbecco's modified Eagle's medium-F-1 2 without serum and supplemented with insulin
C Schmidhauser (1990)
Disruption of epithelial cell-matrix interactions induces apoptosis
S. Frisch (1994)
CA-21765, and CA-13539 from the National Cancer Institute, DHHS; by REFERENCES AND NOTES
Multi‐faceted regulation of cell differentiation by extracellular matrix
C. Lin (1993)
The cells were then lysed hypotonically and removed
E Reichmann (1989)
Cell death by apoptosis during involution of the lactating breast in mice and rats.
N. Walker (1989)
Inhibition of human skin fibroblast collagenase, thermolysin, and Pseudomonas aeruginosa elastase by peptide hydroxamic acids.
D. Grobelny (1992)
A novel heterodimeric cysteine protease is required for interleukin-1βprocessing in monocytes
Nancy A. Thornberry (1992)
Regulation of differentiation and polarized secretion in mammary epithelial cells maintained in culture: extracellular matrix and membrane polarity influences
G. Parry (1987)
Targeted expression of stromelysin-1 in mammary gland provides evidence for a role of proteinases in branching morphogenesis and the requirement for an intact basement membrane for tissue-specific gene expression [published erratum appears in J Cell Biol 1996 Feb;132(4):following 752]
C. J. Sympson (1994)
cal analysis we detected an insoluble, basally deposited ECM rich in type IV collagen and laminin
Science Vol (1995)
Influence of a reconstituted basement membrane and its components on casein gene expression and secretion in mouse mammary epithelial cells.
M. Li (1987)
The GM6001 peptide metalloproteinase inhibitor
designated M2 (14) into the Not site of the pOPRSV vector. This vector contains RSV long terminal repeat (LTR) sequences upstream of an intron sequence with lac repressor binding sites
R Sanchez-Lopez (1988)
Extracellular matrix and hormones transcriptionally regulate bovine beta-casein 5' sequences in stably transfected mouse mammary cells.
C. Schmidhauser (1990)
Synthesis of extracellular matrix glycoproteins by cultured microvascular endothelial cells isolated from the dermis of neonatal and adult skin
Randall H. Kramer (1985)
Coordinated expression of extracellular matrix-degrading proteinases and their inhibitors regulates mammary epithelial function during involution
R. Talhouk (1992)
Chromatin condensation was detected by staining with acridine orange (10 gg/mI) dissolved in phosphatebuffered saline
Prevention of vertebrate neuronal death by the crmA gene.
V. Gagliardini (1994)
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation
Y. Gavrieli (1992)
Integral Membrane Glycoproteins Related to Cell–Substratum Adhesion in Mammalian Cells
C. Damsky (1982)
Inactivated BACMK or tosyl-asparginine-methyl ester (Sigma) (2 to 5 [uM) in dimethyl sulfoxide was used as a control
Lg/mI), and prolactin (3 ptg/ml) at a density of 3.8 x 106 cells per 100 mm plastic culture plates for 4 to 10 days. When indicated
Apoptotic cell death and tissue remodelling during mouse mammary gland involution.
R. Strange (1992)
This paper is referenced by
Bovine pregnancy associated glycoproteins can alter selected transcripts in bovine endometrial explants.
Rhianna M Wallace (2019)
Nanogrooves and keratin nanofibers on titanium surfaces aimed at driving gingival fibroblasts alignment and proliferation without increasing bacterial adhesion.
S. Ferraris (2017)
Interleukin 1 and interleukin 1β converting enzyme (caspase 1) expression in the human colonic epithelial barrier. Caspase 1 downregulation in colon cancer
A. Jarry (1999)
Arabinogalactan-proteins in the Multiple Domains of the Plant Cell Surface
M. D. Serpe (1999)
Psoriasis and apoptosis: A fundamental analysis of the psoriatic phenotype with clinical and therapeutic correlations
C. A. Raskin (1999)
Matrix Metalloproteinases in Remodeling of the Normal and Neoplastic Mammary Gland
L. Rudolph-Owen (2004)
Understanding micrometastatic disease and Anoikis resistance in ewing family of tumors and osteosarcoma.
S. Strauss (2010)
Vascular integrin immunoreactivity is selectively lost on capillaries during rat focal cerebral ischemia and reperfusion
D. Burggraf (2008)
Angiogenesis and matrix metalloproteinases in non-small cell lung cancer
G. Cox (2000)
Proteases and Their Inhibitors in Cancer Metastasis
R. Ablin (2002)
Amniotic membrane transplantation for partial limbal stem cell deficiency
D. Anderson (2001)
Thymic nurse cells: a microenvironment for thymocyte development and selection.
J. Guyden (2003)
The results of amniotic membrane transplantation for primary pterygium compared with conjunctival autograft.
N. Tananuvat (2004)
Modulation of Thr Phosphorylation of Integrin β1 during Muscle Differentiation*
Seon-Myung Kim (2004)
Small cell lung cancer: the importance of the extracellular matrix.
R. Buttery (2004)
Review Signalling mechanisms of anoikis
M. Zhan (2004)
Signalling mechanisms of anoikis.
M. Zhan (2004)
Human Amniotic Membrane : Hope in Periodontal Regeneration
D. Rana (2016)
Primary prevention of breast cancer by hormone-induced differentiation.
I. Russo (2007)
Hepatocyte transplantation in animal models
A. Weber (2009)
Cell‐generated forces influence the viability, metabolism and mechanical properties of fibroblast‐seeded collagen gel constructs
C. Berry (2009)
In vitro prevention of cell‐mediated xeno‐graft rejection via the Fas/FasL‐pathway in CrmA‐transducted porcine kidney cells
M. Fujino (2001)
Apoptosis in normal and neoplastic mammary gland development
R. Strange (2001)
Reactive Oxygen Species and Antioxidants in Luteal Cell Demise
H. Behrman (1997)
An SH3 domain-containing GTPase-activating protein for Rho and Cdc42 associates with focal adhesion kinase.
J. Hildebrand (1996)
Embigin, a developmentally expressed member of the immunoglobulin super family, is also expressed during regression of prostate and mammary gland.
R. Guenette (1997)
Apoptosis in hormone-responsive malignancies.
S. Denmeade (1997)
Both wild-type and strongly attenuated bovine leukemia viruses protect peripheral blood mononuclear cells from apoptosis.
F. Dequiedt (1997)
Feedback control of milk secretion from milk
M. Peaker (2005)
Intestinal tumorigenesis is suppressed in mice lacking the metalloproteinase matrilysin.
C. L. Wilson (1997)
Roles of the matrix metalloproteinases in mammary gland development and cancer
C. Benaud (2004)
Proteolytic activities that mediate apoptosis.
V. Kidd (1998)See more