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Reconstruction Of An Active Integron Recombination Site After Integration Of A Gene Cassette At A Secondary Site

H. Segal, M. Francia, J. Lobo, G. Elisha
Published 1999 · Biology, Medicine

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ABSTRACT As the site of insertion of the aadB gene cassette on pRAY, from a clinical isolate of Acinetobacter, is almost identical to the preferred site on integrons, the composite 59-base element (59-BE) associated with this cassette is potentially recombinationally active. By using a conduction assay to quantitate site activity, the 59-BE was recognized by integrase with high frequency, indicating that the composite site is recombinationally active.
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