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Evidence For Nitrogen Fixation By “ Dehalococcoides Ethenogenes ” Strain 195

Patrick K. H. Lee, Jianzhong He, Stephen H. Zinder, Lisa Alvarez-Cohen

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ABSTRACT Genome annotation of the chlorinated ethene-respiring “ Dehalococcoides ethenogenes ” strain 195 indicated the presence of a complete nitrogenase operon. Here, results from long-term growth experiments, gene expression, and 15 N 2 -isotope measurements confirm that strain 195 is capable of fixing atmospheric dinitrogen when a defined fixed-nitrogen source such as ammonium is unavailable.