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Outer Cell Surface Components Essential For Fe(III) Oxide Reduction By Geobacter Metallireducens
J. A. Smith, D. Lovley, Pier-Luc Tremblay
Published 2012 · Medicine, Biology
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ABSTRACT Geobacter species are important Fe(III) reducers in a diversity of soils and sediments. Mechanisms for Fe(III) oxide reduction have been studied in detail in Geobacter sulfurreducens, but a number of the most thoroughly studied outer surface components of G. sulfurreducens, particularly c-type cytochromes, are not well conserved among Geobacter species. In order to identify cellular components potentially important for Fe(III) oxide reduction in Geobacter metallireducens, gene transcript abundance was compared in cells grown on Fe(III) oxide or soluble Fe(III) citrate with whole-genome microarrays. Outer-surface cytochromes were also identified. Deletion of genes for c-type cytochromes that had higher transcript abundance during growth on Fe(III) oxides and/or were detected in the outer-surface protein fraction identified six c-type cytochrome genes, that when deleted removed the capacity for Fe(III) oxide reduction. Several of the c-type cytochromes which were essential for Fe(III) oxide reduction in G. metallireducens have homologs in G. sulfurreducens that are not important for Fe(III) oxide reduction. Other genes essential for Fe(III) oxide reduction included a gene predicted to encode an NHL (Ncl-1–HT2A–Lin-41) repeat-containing protein and a gene potentially involved in pili glycosylation. Genes associated with flagellum-based motility, chemotaxis, and pili had higher transcript abundance during growth on Fe(III) oxide, consistent with the previously proposed importance of these components in Fe(III) oxide reduction. These results demonstrate that there are similarities in extracellular electron transfer between G. metallireducens and G. sulfurreducens but the outer-surface c-type cytochromes involved in Fe(III) oxide reduction are different.
This paper references
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
Geobacter: the microbe electric's physiology, ecology, and practical applications.
D. Lovley (2011)
Extracellular electron transfer via microbial nanowires
G. Reguera (2005)
Supercapacitors based on c-type cytochromes using conductive nanostructured networks of living bacteria.
N. Malvankar (2012)
Long-range electron transport to Fe(III) oxide via pili with metallic-like conductivity.
D. Lovley (2012)
Direct Exchange of Electrons Within Aggregates of an Evolved Syntrophic Coculture of Anaerobic Bacteria
Zarath M. Summers (2010)
Current Protocols in Molecular Biology (New York: Greene Publishing Associates and Wiley-Interscience). Host-Range Shuttle System for Gene Insertion into the Chromosomes of Gram-negative Bacteria.
F. M. Ausubel (1988)
Anode Biofilm Transcriptomics Reveals Outer Surface Components Essential for High Density Current Production in Geobacter sulfurreducens Fuel Cells
K. Nevin (2009)
Live wires: direct extracellular electron exchange for bioenergy and the bioremediation of energy-related contamination
D. Lovley (2011)
Molecular cloning: A laboratory manual 2nd ed. Edited by J. . Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989 (in 3 volumes)
P. Deininger (1990)
Molecular Analysis of the O-Antigen Gene Cluster of Escherichia coli O86:B7 and Characterization of the Chain Length Determinant Gene (wzz)
Hongjie Guo (2005)
Potential for Direct Interspecies Electron Transfer in Methanogenic Wastewater Digester Aggregates
M. Morita (2011)
Evidence that OmcB and OmpB of Geobacter sulfurreducens are outer membrane surface proteins.
Xinlei Qian (2007)
Potential for Quantifying Expression of the Geobacteraceae Citrate Synthase Gene To Assess the Activity of Geobacteraceae in the Subsurface and on Current-Harvesting Electrodes
D. Holmes (2005)
Transcriptome of Geobacter uraniireducens growing in uranium-contaminated subsurface sediments
D. Holmes (2009)
Electrical Conductivity in a Mixed-Species Biofilm
N. Malvankar (2012)
Alignment of the c-Type Cytochrome OmcS along Pili of Geobacter sulfurreducens
C. Leang (2010)
The structure of the core region of the lipopolysaccharide from Geobacter sulfurreducens.
E. Vinogradov (2004)
Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria.
C. J. Marx (2001)
Genome-scale analysis of anaerobic benzoate and phenol metabolism in the hyperthermophilic archaeon Ferroglobus placidus
D. Holmes (2012)
Outer Membrane c-Type Cytochromes Required for Fe(III) and Mn(IV) Oxide Reduction in Geobacter sulfurreducens
T. Mehta (2005)
Identification of Multicomponent Histidine-Aspartate Phosphorelay System Controlling Flagellar and Motility Gene Expression in Geobacter Species*
T. Ueki (2012)
Characterization of the Escherichia coli O59 and O155 O-antigen gene clusters: the atypical wzx genes are evolutionary related.
H. Guo (2005)
Geobacter metallireducens gen. nov. sp. nov., a microorganism capable of coupling the complete oxidation of organic compounds to the reduction of iron and other metals
D. Lovley (2004)
Biofilm and Nanowire Production Leads to Increased Current in Geobacter sulfurreducens Fuel Cells
G. Reguera (2006)
DR Lovley (2012)
Pseudomonas aeruginosa 1244 pilin glycosylation: glycan substrate recognition.
Joseph Horzempa (2006)
MacA, a diheme c-type cytochrome involved in Fe(III) reduction by Geobacter sulfurreducens.
J. Butler (2004)
Specific localization of the c-type cytochrome OmcZ at the anode surface in current-producing biofilms of Geobacter sulfurreducens.
K. Inoue (2011)
Anaerobic Benzene Oxidation by Geobacter Species
Tian Zhang (2012)
Functional characterization of bacterial oligosaccharyltransferases involved in O-linked protein glycosylation.
Amirreza Faridmoayer (2007)
An improved staining procedure for the detection of the peroxidase activity of cytochrome P-450 on sodium dodecyl sulfate polyacrylamide gels.
P. Thomas (1976)
Purification and Characterization of OmcZ, an Outer-Surface, Octaheme c-Type Cytochrome Essential for Optimal Current Production by Geobacter sulfurreducens
K. Inoue (2010)
Development of a Genetic System forGeobacter sulfurreducens
M. Coppi (2001)
PSORTb 3.0: improved protein subcellular localization prediction with refined localization subcategories and predictive capabilities for all prokaryotes
N. Y. Yu (2010)
Microbial nanowires: a new paradigm for biological electron transfer and bioelectronics.
N. Malvankar (2012)
The immunoglobulin fold. Structural classification, sequence patterns and common core.
P. Bork (1994)
Tunable metallic-like conductivity in microbial nanowire networks.
N. Malvankar (2011)
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
K. Livak (2001)
Analysis of Shigella flexneri Wzz (Rol) function by mutagenesis and cross‐linking: Wzz is able to oligomerize
C. Daniels (1999)
Identification of an extracellular polysaccharide network essential for cytochrome anchoring and biofilm formation in Geobacter sulfurreducens.
Janet B Rollefson (2011)
A genetic system for Geobacter metallireducens: role of the flagellin and pilin in the reduction of Fe(III) oxide.
Pier-Luc Tremblay (2012)
Cyclic voltammetry of biofilms of wild type and mutant Geobacter sulfurreducens on fuel cell anodes indicates possible roles of OmcB, OmcZ, type IV pili, and protons in extracellular electron transfer
H. Richter (2009)
Biochemical and genetic characterization of PpcA, a periplasmic c-type cytochrome in Geobacter sulfurreducens.
J. Lloyd (2003)
Evolution of electron transfer out of the cell: comparative genomics of six Geobacter genomes
J. Butler (2009)
16S ribosomal DNA amplification for phylogenetic study.
William G. Weisburg (1991)
Naphthalene and Benzene Degradation under Fe(III)-Reducing Conditions in Petroleum-Contaminated Aquifers
R. T. Anderson (1999)
Geobacter metallireducens accesses insoluble Fe(iii) oxide by chemotaxis
S. Childers (2002)
The genome sequence of Geobacter metallireducens: features of metabolism, physiology and regulation common and dissimilar to Geobacter sulfurreducens
M. Aklujkar (2008)
Microarray and genetic analysis of electron transfer to electrodes in Geobacter sulfurreducens.
D. Holmes (2006)
Identification of genes involved in biofilm formation and respiration via mini-Himar transposon mutagenesis of Geobacter sulfurreducens.
Janet B Rollefson (2009)
Molecular characterization of the Pseudomonas aeruginosa serotype O5 (PAO1) B‐band lipopolysaccharide gene cluster
L. Burrows (1996)
This paper is referenced by
Proteome of Geobacter sulfurreducens in the presence of U(VI).
Roberto Orellana (2014)
Constraint-Based Modeling of Carbon Fixation and the Energetics of Electron Transfer in Geobacter metallireducens
A. Feist (2014)
U(VI) Reduction by a Diversity of Outer Surface C-Type Cytochromes of Geobacter 1
Kelly A. Flanagan (2013)
Combined Biological and Chemical Mechanisms for Degradation of Insensitive Munitions
Jolanta B. Niedźwiecka (2016)
Anaerobic Utilization of Hydrocarbons, Oils, and Lipids
Matthias Boll (2020)
Functional characterization and directed engineering of redox proteins for a rational improvement of Bioelectrochemical systems
A. Alves (2014)
Bacterially facilitated rock-coating formation as a component of the geochemical budget in cold climates: An example from Kärkevagge, Swedish Lapland
C. L. Marnocha (2014)
Physiology of Geobacter metallireducens under excess and limitation of electron donors. Part II. Mimicking environmental conditions during cultivation in retentostats.
S. Marozava (2014)
Mechanisms Involved in Fe(III) Respiration by the Hyperthermophilic Archaeon Ferroglobus placidus
J. A. Smith (2015)
metallireducens and Methanosarcina barkeri 2
Amelia-Elena Rotaru (2014)
Further Readings in Geomicrobiology
The Family Geobacteraceae
W. Röling (2014)
Characterization of extracellular electron transfer components of Geobacter bacteria
T. Fernandes (2018)
Microbial nanowires for bioenergy applications.
N. Malvankar (2014)
The electrically conductive pili of Geobacter soli
Shiyan Zhuo (2020)
A Chemist's Perspective on Bioenergy: Opportunities and Challenges
Sannapaneni Janardan (2015)
Extracellular Electron Uptake: Among Autotrophs and Mediated by Surfaces.
Pier-Luc Tremblay (2017)
Mediation of Extracellular Polymeric Substances in Microbial Reduction of Hematite by Shewanella oneidensis MR-1
L. Gao (2019)
A comprehensive overview on electro-active biofilms, role of exo-electrogens and their microbial niches in microbial fuel cells (MFCs).
G. Saratale (2017)
PHYSIOLOGICAL MODELS OF GEOBACTER SULFURREDUCENS AND DESULFOBACTER POSTGATEI TO UNDERSTAND URANIUM REMEDIATION IN SUBSURFACE SYSTEMS
R. Orellana (2014)
Novel insights into the taxonomic diversity and molecular mechanisms of bacterial Mn(III) reduction
Nadia Szeinbaum (2019)
Methanosarcina barkeri between Geobacter metallireducens and Direct Interspecies Electron Transfer
Derek R. Lovley (2014)
Redox cycling of Fe(II) and Fe(III) in magnetite by Fe-metabolizing bacteria
J. Byrne (2015)
Going Wireless: Fe(III) Oxide Reduction without Pili by Geobacter sulfurreducens Strain JS-1
J. A. Smith (2014)
Anodic concentration loss and impedance characteristics in rotating disk electrode microbial fuel cells
Liye Shen (2016)
Global transcriptional analysis of Geobacter sulfurreducens under palladium reducing conditions reveals new key cytochromes involved
A. Hernández-Eligio (2018)
Iron Oxide Nanoparticles for Biomedical Applications: A Perspective on Synthesis, Drugs, Antimicrobial Activity, and Toxicity
L. S. Arias (2018)
Mechanisms for Extracellular Electron Exchange by Geobacter Species
J. A. Smith (2015)
Exoelectrogens: Recent Advances in Molecular Drivers Involved in Extracellular Electron Transfer and Strategies used to Improve it for Microbial Fuel Cell Applications
R. Kumar (2016)
Iron and Electron Shuttle Mediated (Bio)degradation of 2,4-Dinitroanisole (DNAN).
Jolanta B. Niedźwiecka (2017)
Recovery of Metals from Wastes Using Bioelectrochemical Systems
Liping Huang (2019)
Transcriptomic and Genetic Analysis of Direct Interspecies Electron Transfer
P. Shrestha (2013)See more