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Characterization Of Benthic Microbial Community Structure By High-resolution Gas Chromatography Of Fatty Acid Methyl Esters.

R. J. Bobbie, D. White
Published 1980 · Chemistry, Medicine

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Fatty acids are a widely studied group of lipids of sufficient taxonomic diversity to be useful in defining microbial community structure. The extraordinary resolution of glass capillary gas-liquid chromatography can be utilized to separate and tentatively identify large numbers of fatty acid methyl esters derived from the lipids of estuarine detritus and marine benthic microbiota without the bias of selective methods requiring culture or recovery of the microbes. The gas-liquid chromatographic analyses are both reproducible and highly sensitive, and the recovery of fatty acids is quantitative. The analyses can be automated, and the diagnostic technique of mass spectral fragmentation analysis can be readily applied. Splitless injection on glass capillary gas chromatographic columns detected by mass spectral selective ion monitoring provides an ultrasensitive and definitive monitoring system. Reciprocal mixtures of bacteria and fungi, when extracted and analyzed, showed progressive changes of distinctive fatty acid methyl esters derived from the lipids. By manipulating the environment of an estuarine detrital microbial community with antibiotics and culture conditions, it was possible to produce a community greatly enriched in eucaryotic fungi, as evidenced by scanning electron microscopic morphology. The fatty acid methyl esters from the lipids in the fungus-enriched detritus showed enrichment of the C(18) dienoic and the C(18) and C(20) polyenoic esters. Manipulation of the detrital microbiota that increased the procaryotic population resulted in an absence of large structures typical of fungal mycelia or diatoms, as evidenced by scanning electron microscopy, and a significantly larger proportion of anteiso- and isobranched C(15) fatty acid esters, C(17) cyclopropane fatty acid esters, and the cis-vaccenic isomer of the C(18) monoenoic fatty acid esters. As determined by these techniques, a marine settling community showed greater differences in bacterial as contrasted to microeucaryotic populations when compared with the microbial communities of benthic cores.
This paper references

This paper is referenced by
Characterization of microbial populations in polluted marine sediments
R. J. Parkes (1985)
A new method for assessing soil microorganism diversity and evidence of vitamin deficiency in low diversity communities
J. Korner (2004)
Carbon source utilization of soil extracted microorganisms as a tool to detect the effects of soil supplemented with genetically engineered and non-engineered Corynebacterium glutamicum and a recombinant peptide at the community level
W. Vahjen (1995)
Isolation and characterization of antibiotic producing thermophilic bacillus in selected hot-springs along lake Bogoria, Kenya
Tom Kintet Torome (2015)
Chemical Characterization of Films
D. White (1984)
Discovery of marine Bacillus species by 16S rRNA and rpoB comparisons and their usefulness for species identification.
J. Ki (2009)
Identification and ecology of bacterial communities associated with necroses of three cactus species.
J. Foster (1993)
Partial characterization of organic material in surface sediments from Balsfjorden, northern Norway, in relation to its origin and nutritional value for sediment-ingesting animals
J. S. Sargent (1983)
Determination of the gram-positive bacterial content of soils and sediments by analysis of teichoic acid components.
M. J. Gehron (1984)
A quantitative comparison of microbial community structure of estuarine sediments from microcosms and the field
T. Federle (1986)
Molecular Methods for Studying Microorganisms From Atypical Environments
H. R. Dash (2018)
Development of a group-specific PCR combined with ARDRA for the identification of Bacillus species of environmental significance.
Xiyang Wu (2006)
Microbial community structure in sediments of a polluted bay as determined by phospholipid ester-linked fatty acids
N. Rajendran (1992)
Influence of n-alkanes and petroleum on fatty acid composition of a hydrocarbonoclastic bacterium: Marinobacter hydrocarbonoclasticus strain 617.
P. Doumenq (2001)
Plasmalogens of microbial communities associated with barley straw and clover in the rumen
L. Saluzzi (1995)
Anaerobic microbial activities including hydrogen mediated acetogenesis within natural gas transmission lines
T. Phelps (1991)
C15, C20, and C25 isoprenoid homologues in glycerol diether phospholipids of methanogenic archaebacteria.
C. Mancuso (1985)
Determination of factors controlling the fatty acid composition of settling particles in the water column by principal-component analysis and their quantitative assessment by multiple regression
T. Reemtsma (1992)
Incorporation and utilization of bacterial lipids in theSolemya velum symbiosis
N. Conway (1991)
The effects of feeding by the sand dollar Mellita quinquiesperforata (Leske) on the benthic microbial community
R. Findlay (1983)
Bacillus aequororis sp. nov., Isolated From Marine Sediment
N. Singh (2014)
Minocycline Down-Regulates Topical Mucosal Inflammation during the Application of Microbicide Candidates
L. Li (2012)
From gene expression to genetic adaptation : insights into the spatio-temporal dynamics of Alexandrium minutum cryptic species complex
Gabriel Metegnier (2018)
Microbial signature lipid profiling and exopolysaccharides: experiences initiated with Professor David C White and transported to Tasmania, Australia.
P. Nichols (2008)
Fatty Acids in Liver, Muscle and Gonad of three Tropical Rays including Non-Methylene-Interrupted Dienoic Fatty Acids
Mohamed Vall Ould El Kebir (2007)
Modifications of estuarine sedimentary microbiota by exclusion of epibenthic predators
T. Federle (1983)
Effects of oil and gas well-drilling fluids on the biomass and community structure of microbiota that colonize sands in running seawater
G. A. Smith (1982)
Controls on soil nitrogen cycling and microbial community composition across land use and incubation temperature
W. Cookson (2007)
Phospholipid fatty acid profiling of microbial communities–a review of interpretations and recent applications
C. Willers (2015)
Perturbations in the biomass, metabolic activity, and community structure of the estuarine detrital microbiota: Resource partitioning in amphipod grazing
G. A. Smith (1982)
The Behavior and Tracking of Bacteria in the Rhizosphere
D. Kluepfel (1993)
icrobial community analysis of perchlorate-reducing cultures rowing on zero-valent iron
hjeong Sona (2010)
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