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Utility Of Green Fluorescent Nucleic Acid Dyes And Aluminum Oxide Membrane Filters For Rapid Epifluorescence Enumeration Of Soil And Sediment Bacteria

Markus G. Weinbauer, Christiane Beckmann, Manfred G. Höfle

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ABSTRACT High background fluorescence and unspecific staining hampered the epifluorescence enumeration of bacteria in 45% of the tested soil and sediment samples with 4′,6-diamidino-2-phenylindole (DAPI) and polycarbonate membrane filters. These problems of the determination of total cell counts can be circumvented by using green fluorescent high-affinity nucleic acid dyes and aluminum oxide membrane filters. Due to the bright staining of cells, we recommend SYBR Green II as dye.