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Ink And Vinegar, A Simple Staining Technique For Arbuscular-Mycorrhizal Fungi

Horst Vierheilig, Andrew P. Coughlan, Urs Wyss, Yves Piché

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ABSTRACT We developed a reliable, inexpensive, and simple method for staining arbuscular-mycorrhizal fungal colonizations in root tissues. Apart from applications in research, this nontoxic, high-quality staining method also could be of great utility in teaching exercises. After adequate clearing with KOH, an ink-vinegar solution successfully stained all fungal structures, rendering them clearly visible.