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CumA, A Gene Encoding A Multicopper Oxidase, Is Involved In Mn2+ Oxidation In Pseudomonas Putida GB-1

Geert-Jan Brouwers, Johannes P. M. de Vrind, Paul L. A. M. Corstjens, Pierre Cornelis, Christine Baysse, Elisabeth W. de Vrind-de Jong

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ABSTRACT Pseudomonas putida GB-1-002 catalyzes the oxidation of Mn2+. Nucleotide sequence analysis of the transposon insertion site of a nonoxidizing mutant revealed a gene (designatedcumA) encoding a protein homologous to multicopper oxidases. Addition of Cu2+ increased the Mn2+-oxidizing activity of the P. putidawild type by a factor of approximately 5. The growth rates of the wild type and the mutant were not affected by added Cu2+. A second open reading frame (designatedcumB) is located downstream from cumA. BothcumA and cumB probably are part of a single operon. The translation product of cumB was homologous (level of identity, 45%) to that of orf74 ofBradyrhizobium japonicum. A mutation in orf74resulted in an extended lag phase and lower cell densities. Similar growth-related observations were made for the cumAmutant, suggesting that the cumA mutation may have a polar effect on cumB. This was confirmed by site-specific gene replacement in cumB. The cumB mutation did not affect the Mn2+-oxidizing ability of the organism but resulted in decreased growth. In summary, our data indicate that the multicopper oxidase CumA is involved in the oxidation of Mn2+ and that CumB is required for optimal growth of P. putida GB-1-002.