Endophytic Colonization And In Planta Nitrogen Fixation By A Herbaspirillum Sp. Isolated From Wild Rice Species
A. El-beltagy, K. Nishioka, T. Sato, H. Suzuki, B. Ye, T. Hamada, T. Isawa, H. Mitsui, K. Minamisawa
Published 2001 · Biology, Medicine
Download PDFAnalyze on Scholarcy
ABSTRACT Nitrogen-fixing bacteria were isolated from the stems of wild and cultivated rice on a modified Rennie medium. Based on 16S ribosomal DNA (rDNA) sequences, the diazotrophic isolates were phylogenetically close to four genera: Herbaspirillum,Ideonella, Enterobacter, andAzospirillum. Phenotypic properties and signature sequences of 16S rDNA indicated that three isolates (B65, B501, and B512) belong to the Herbaspirillum genus. To examine whether Herbaspirillum sp. strain B501 isolated from wild rice, Oryza officinalis, endophytically colonizes rice plants, the gfp gene encoding green fluorescent protein (GFP) was introduced into the bacteria. Observations by fluorescence stereomicroscopy showed that the GFP-tagged bacteria colonized shoots and seeds of aseptically grown seedlings of the original wild rice after inoculation of the seeds. Conversely, for cultivated rice Oryza sativa, no GFP fluorescence was observed for shoots and only weak signals were observed for seeds. Observations by fluorescence and electron microscopy revealed that Herbaspirillum sp. strain B501 colonized mainly intercellular spaces in the leaves of wild rice. Colony counts of surface-sterilized rice seedlings inoculated with the GFP-tagged bacteria indicated significantly more bacterial populations inside the original wild rice than in cultivated rice varieties. Moreover, after bacterial inoculation, in planta nitrogen fixation in young seedlings of wild rice, O. officinalis, was detected by the acetylene reduction and 15N2gas incorporation assays. Therefore, we conclude thatHerbaspirillum sp. strain B501 is a diazotrophic endophyte compatible with wild rice, particularly O. officinalis.
This paper references
The growing rice plant : an anatomical monograph
星川 清親 (1989)
The neighbor-joining method: a new method for reconstructing phylogenetic trees.
Naruya Saitou (1987)
Comparison of benefit to sugarcane plant growth and 15N2 incorporation following inoculation of sterile plants with Acetobacter diazotrophicus wild-type and Nif- mutants strains.
M. Sevilla (2001)
Ideonella dechloratans gen.nov., sp.nov., a New Bacterium Capable of Growing Anaerobically with Chlorate as an Electron Acceptor
Åsa Malmqvist (1994)
Effect of inoculation with Klebsiella oxytoca and Enterobacter cloacae on dinitrogen fixation by rice-bacteria associations. Plant Soil 103:221–226
T. Fujie (1987)
Bacterial Endophytes: Potential Role in Developing Sustainable Systems of Crop Production
A. V. Sturz (2000)
GFP as a marker gene
A. Unge (1998)
Non-nodular endorhizospheric nitrogen fixation in wetland rice
Chongbiao You (1989)
Life in grasses: diazotrophic endophytes.
B. Reinhold-Hurek (1998)
Isolation of endophytic bacteria from wetland rice
W. L. Barraquio (1997)
Infection of sugar cane by the nitrogen-fixing bacterium Acetobacter diazotrophicus
E. James (1994)
Emended description of Herbaspirillum; inclusion of [Pseudomonas] rubrisubalbicans, a milk plant pathogen, as Herbaspirillum rubrisubalbicans comb. nov.; and classification of a group of clinical isolates (EF group 1) as Herbaspirillum species 3.
J. I. Baldani (1996)
16S ribosomal DNA amplification for phylogenetic study.
William G. Weisburg (1991)
A single medium for the isolation of acetylene-reducing (dinitrogen-fixing) bacteria from soils.
R. J. Rennie (1981)
Effect of inoculation withKlebsiella oxytoca andEnterobacter cloacae on dinitrogen fixation by rice-bacteria associations
T. Fujii (1987)
Phenotypic and genetic diversity of chlorine-resistant Methylobacterium strains isolated from various environments.
Akira Hiraishi (1995)
Ecological-Genetic Studies on Wild and Cultivated Rice in Tropical Asia (4th survey). 2. Wild Rice in the Middle Basin of Mekong River.
Hiroshi Yamagishi (1994)
Isolation and characterization of endophytic bacteria from wild and traditionally cultivated rice varieties
A. El-beltagy (2000)
Infection and Colonization of Sugar Cane and Other Graminaceous Plants by Endophytic Diazotrophs
E. James (1998)
The Remobilization of Nitrogen Related to Leaf Growth and Senescence in Rice Plants (Oryza sativa L.)
T. Mae (1981)
Use of green fluorescent protein to detect expression of nif genes of Azoarcus sp. BH72, a grass-associated diazotroph, on rice roots.
T. Egener (1998)
Root colonization and systemic spreading of Azoarcus sp. strain BH72 in grasses.
T. Hurek (1994)
The genus Serratia, p. 2822–2848
F. Grimont (1991)
Molecular Basis of Symbiotic Promiscuity
X. Perret (2000)
Characterization of Herbaspirillum seropedicae gen. nov., sp. nov., a root-associated nitrogen-fixing bacterium.
J. I. Baldani (1986)
Herbaspirillum frisingense sp. nov., a new nitrogen-fixing bacterial species that occurs in C4-fibre plants.
G. Kirchhof (2001)
Manual of Methods for General Bacteriology
D. Jones (1981)
Preferential occurrence of diazotrophic endophytes, Azoarcus spp., in wild rice species and land races of Oryza sativa in comparison with modern races.
M. Engelhard (2000)
Nitrogen fixation in endophytic and associative symbiosis.
E. James (2000)
Host-plant specificity in the infection of cereals with Azospirillum spp
V. L. Baldani (1980)
Biological Nitrogen Fixation in Sugar Cane: A Key to Energetically Viable Biofuel Production
R. Boddey (1995)
Origin of cultivated rice
H. Oka (1988)
Phylogeny of spore-forming lactic acid bacteria based on 16S rRNA gene sequences.
Taiga Suzuki (1994)
DoesAcetobacter diazotrophicusLive and Move in the Xylem of Sugarcane Stems? Anatomical and Physiological Data
Z. Dong (1997)
Herbaspirillum, an endophytic diazotroph colonizing vascular tissue in leaves of Sorghum bicolor L. Moench
E. James (1997)
Recent advances in BNF with non-legume plants
J. Baldani (1997)
The potential role of biological nitrogen fixation in meeting future demand for rice and fertilizer
D. Dawe (2000)
The quest for nitrogen fixation in rice.
J. K. Ladha (2000)
This paper is referenced by
A Bacterial Endophyte, Pseudomonas brassicacearum YC5480, Isolated from the Root of Artemisia sp. Producing Antifungal and Phytotoxic Compounds
Bok Sil Chung (2008)
Effects of rhizobial inoculation, cropping systems and growth stages on endophytic bacterial community of soybean roots
Yun Zhang (2011)
Biodiversity of diazotrophic bacteria within the soil, root and stem of field-grown maize
Luiz Fernando Würdig Roesch (2007)
Endophytic Lifestyle of Biocontrol Strains of Pseudomonas spp. in Olive Roots
Jesús Mercado-Blanco (2013)
Effects of Colonization of a Bacterial Endophyte, Azospirillum sp. B510, on Disease Resistance in Rice
Michiko Yasuda (2009)
The role of the antimicrobial compound 2,4-diacetylphloroglucinol in the impact of biocontrol Pseudomonas fluorescens F113 on Azospirillum brasilense phytostimulators.
Olivier Couillerot (2011)
Instructions for use Title Identification and isolation of active N 2 O reducers in rice paddy soil
Satoshi Ishii (2017)
The art of isolating nitrogen-fixing bacteria from non-leguminous plants using N-free semi-solid media: a practical guide for microbiologists
José Ivo Baldani (2014)
Phylogenyofthe 1-aminocyclopropane-1-carboxylic acid deaminase-encodinggeneacdS in phytobene ¢ cial and pathogenic Proteobacteria and relationwith strain biogeography
Didier Blaha (2006)
Beneficial microbiomes: Biodiversity and potential biotechnological applications for sustainable agriculture and human health -
Ajar Nath Yadav (2017)
Determination of the bacterial diversity of a natural freshwater wetland impacted by acid mine drainage.
Karin Staebe (2015)
Selección de cepas de Herbaspirillum spp. promotoras del crecimiento de arroz
Karina Punschke (2011)
Isolation and Identification Enterobacter asburiae from Consumed Powdered Infant Formula Milk (PIF) in the Neonatal Intensive Care Unit (NICU).
J. Mardaneh (2016)
Doses and application methods of Azospirillum brasilense in irrigated upland rice
Nayara Fernanda Siviero Garcia (2016)
Production of border cells and colonization of maize root tips by Herbaspirillum seropedicae are modulated by humic acid
Luciano Pasqualoto Canellas (2017)
The influence of host genotype and salt stress on the seed endophytic community of salt-sensitive and salt-tolerant rice cultivars
Denver I Walitang (2018)
Endophytic microorganisms of tropical tuber crops: Potential and perspectives
Shubhransu Nayak (2017)
Antagonism between Azospirillum brasilense Az 39 and Pseudomonas oryzihabitans , a seed-borne endophyte , in growing rice plants
G. Rariz (2017)
Modulation of defence and iron homeostasis genes in rice roots by the diazotrophic endophyte Herbaspirillum seropedicae
L. Brusamarello-Santos (2019)
Growth and biochemical profiling of artificially associated micropropagated oil palm plantlets with Herbaspirillum seropedicae
Shey-Li Lim (2018)
Evaluation of diazotrophic bacteria as biofertilizers.
M. H. Kifle (2014)
The Type III Secretion System (T3SS) is a Determinant for Rice-Endophyte Colonization by Non-Photosynthetic Bradyrhizobium
Pongdet Piromyou (2015)
Applicability of the 16S-23S rDNA internal spacer for PCR detection of the phytostimulatory PGPR inoculant Azospirillum lipoferum CRT1 in field soil.
E. Baudoin (2010)
Protist-enhanced survival of a plant growth promoting rhizobacteria, Azospirillum sp. B510, and the growth of rice (Oryza sativa L.) plants
Rasit Asiloglu (2020)
Plasmid Plasticity in the Plant-Associated Bacteria of the Genus Azospirillum
Elena I. Katsy (2011)
Rice （Oryza sativa L.） nutrient management using mycorrhizal fungi and endophytic Herbaspirillum seropedicae
Hamzeh Hoseinzade (2016)
Azospirillum sp. in current agriculture: From the laboratory to the field
Fabricio Cassán (2016)
Influence of External Nitrogen on Nitrogenase Enzyme Activity and Auxin Production in Herbaspirillum seropedicae (Z78).
Tan Tzy Yin (2015)
Azospirillum Changes in Phase Variation and Genomic Architecture
Ludovic Vial (2006)
Endophytic Bacteria in the Rice Plant
Transmission of Bacterial Endophytes
Anna Carolin Frank (2017)
The type III secretion system is necessary for the development of a pathogenic and endophytic interaction between Herbaspirillum rubrisubalbicans and Poaceae
M. A. Schmidt (2011)See more