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Direct Production Of Ethanol From Raw Corn Starch Via Fermentation By Use Of A Novel Surface-Engineered Yeast Strain Codisplaying Glucoamylase And α-Amylase

H. Shigechi, J. Koh, Y. Fujita, T. Matsumoto, Yohei Bito, M. Ueda, E. Satoh, H. Fukuda, A. Kondo
Published 2004 · Biology, Medicine

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ABSTRACT Direct and efficient production of ethanol by fermentation from raw corn starch was achieved by using the yeast Saccharomyces cerevisiae codisplaying Rhizopus oryzae glucoamylase and Streptococcus bovis α-amylase by using the C-terminal-half region of α-agglutinin and the flocculation functional domain of Flo1p as the respective anchor proteins. In 72-h fermentation, this strain produced 61.8 g of ethanol/liter, with 86.5% of theoretical yield from raw corn starch.
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