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Mouse Strain-dependent Variation In The Course And Outcome Of Chlamydial Genital Tract Infection Is Associated With Differences In Host Response

T. Darville, C. Andrews, K. Laffoon, W. Shymasani, L. R. Kishen, R. Rank
Published 1997 · Medicine, Biology

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Whether there is a pathogenic or protective outcome to chlamydial infection may be defined by the host response. We infected C57BL/6 (C57) and C3H/HeN (C3H) mice with the human biovar of Chlamydia trachomatis, serovar E, and, in select experiments, with the mouse pneumonitis agent of C. trachomatis (MoPn). We compared the courses of infection, histopathology, and host responses that resulted from these infections. The duration of infection with either chlamydial biovar was significantly increased in the C3H strain of mice. The intensity of infection was examined in mice infected with serovar E, and it was significantly increased in the C3H strain. Histopathology revealed the incidence of severe hydrosalpinx to be significantly greater in C3H mice than in C57 mice. In contrast, severe distention of the uterine horns was observed in all infected C57 mice compared to none of the C3H mice infected with serovar E and only 25% of those infected with MoPn. Acute inflammation was significantly increased in the uterine horns of C57 mice compared to that of C3H mice. Examination of antigen-specific responses revealed qualitatively similar responses in the two strains. Determination of gamma interferon- versus interleukin 4- producing cells revealed the predominance of a Th1 response in both strains. Serum enzyme-linked immunosorbent assays for immunoglobulin G1 (IgG1) and IgG2a revealed a predominance of IgG2a antibody in both strains, although the levels of antibody were significantly greater in C3H mice. Lymphocyte proliferation studies revealed increased proliferation in the iliac nodes of both strains at 1 to 3 weeks after infection. Because of the early eradication of infection observed in the C57 strain, we explored the relative production of tumor necrosis factor alpha (TNF-alpha) in the two strains. TNF-alpha levels were significantly increased in the genital tract secretions of C57 mice compared to that of C3H mice during the first week of infection. Increased TNF-alpha may be beneficial to the host by leading to earlier eradication of infection, thereby preventing infection of the oviduct and thus the major disease sequelae associated with chlamydial infection of the genital tract.
This paper references
Manual of clinical microbiology
P. Murray (1975)
Chlamydia trachomatis infection of the Fallopian tubes. Histological findings in two patients.
B. Møller (1979)
Chlamydiae (psittacosis-lymphogranuloma venereumtrachoma group), p. 357–364
J. Schachter (1980)
Chlamydiae ( psittacosis - lymphogranuloma venereum - trachoma group )
E. H. Lennette (1980)
Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis
H. Caldwell (1981)
Salpingitis in mice induced by human strains of Chlamydia trachomatis.
M. Tuffrey (1986)
The in vitro bactericidal activity of peritoneal and spleen cells from Listeria-resistant and -susceptible mouse strains.
P. Wood (1986)
Genetic control of responsiveness of mice to Chlamydia trachomatis pneumonitis
D. M. Graham (1986)
Genetic control of responsiveness of mice to Chlamydia trachomatis pneumonitis , p . 396
T. Triglia (1986)
Effect of Prior Sexually Transmitted Disease on the Isolation of Chlamydia trachomatis
B. Katz (1987)
Chlamydia trachomatis infections in the United States. What are they costing us?
A. Washington (1987)
Variation in virulence among oculogenital serovars of Chlamydia trachomatis in experimental genital tract infection
J. Ito (1990)
A role in vivo for tumor necrosis factor alpha in host defense against Chlamydia trachomatis
D. Williams (1990)
Genetic control of natural resistance to Chlamydia psittaci loth strain in mice
B Fuentes (1990)
Genetic control of natural resistance to Chlamydia psittaci loth strain in mice, p. 232
B. Fuentes (1990)
Genital chlamydial infections: epidemiology and reproductive sequelae.
W. Cates (1991)
Production of colony-stimulating factors during pneumonia caused by Chlamydia trachomatis
D. Magee (1991)
Heterotypic protection of mice against chlamydial salpingitis and colonization of the lower genital tract with a human serovar F isolate of Chlamydia trachomatis by prior immunization with recombinant serovar L1 major outer-membrane protein.
M. Tuffrey (1992)
Chlamydia trachomatis pneumonia induces in vivo production of interleukin-1 and -6
D. Magee (1992)
Intravaginal inoculation of mice with the Chlamydia trachomatis mouse pneumonitis biovar results in infertility
L. M. de la Maza (1994)
Local Th1-like responses are induced by intravaginal infection of mice with the mouse pneumonitis biovar of Chlamydia trachomatis
T. Cain (1995)
Tumor necrosis factor alpha activity in genital tract secretions of guinea pigs infected with chlamydiae
T. Darville (1995)
Mutational analysis of TNF-alpha gene reveals a regulatory role for the 3'-untranslated region in the genetic predisposition to lupus-like autoimmune disease.
C. Jacob (1996)
Initial route of antigen administration alters the T-cell cytokine profile produced in response to the mouse pneumonitis biovar of Chlamydia trachomatis following genital infection
K. Kelly (1996)
Genetically determined differences in IL-10 and IFN-gamma responses correlate with clearance of Chlamydia trachomatis mouse pneumonitis infection.
X. Yang (1996)
Secretion of proinflammatory cytokines by epithelial cells in response to Chlamydia infection suggests a central role for epithelial cells in chlamydial pathogenesis.
S. Rasmussen (1997)

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S. Lehr (2018)
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L. Li (2017)
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Chlamydia trachomatis Genital Tract Infections: When Host Immune Response and the Microbiome Collide.
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