Online citations, reference lists, and bibliographies.
← Back to Search

Mouse Strain-dependent Variation In The Course And Outcome Of Chlamydial Genital Tract Infection Is Associated With Differences In Host Response

T. Darville, C. Andrews, K. Laffoon, W. Shymasani, L. R. Kishen, R. Rank
Published 1997 · Medicine, Biology

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
Whether there is a pathogenic or protective outcome to chlamydial infection may be defined by the host response. We infected C57BL/6 (C57) and C3H/HeN (C3H) mice with the human biovar of Chlamydia trachomatis, serovar E, and, in select experiments, with the mouse pneumonitis agent of C. trachomatis (MoPn). We compared the courses of infection, histopathology, and host responses that resulted from these infections. The duration of infection with either chlamydial biovar was significantly increased in the C3H strain of mice. The intensity of infection was examined in mice infected with serovar E, and it was significantly increased in the C3H strain. Histopathology revealed the incidence of severe hydrosalpinx to be significantly greater in C3H mice than in C57 mice. In contrast, severe distention of the uterine horns was observed in all infected C57 mice compared to none of the C3H mice infected with serovar E and only 25% of those infected with MoPn. Acute inflammation was significantly increased in the uterine horns of C57 mice compared to that of C3H mice. Examination of antigen-specific responses revealed qualitatively similar responses in the two strains. Determination of gamma interferon- versus interleukin 4- producing cells revealed the predominance of a Th1 response in both strains. Serum enzyme-linked immunosorbent assays for immunoglobulin G1 (IgG1) and IgG2a revealed a predominance of IgG2a antibody in both strains, although the levels of antibody were significantly greater in C3H mice. Lymphocyte proliferation studies revealed increased proliferation in the iliac nodes of both strains at 1 to 3 weeks after infection. Because of the early eradication of infection observed in the C57 strain, we explored the relative production of tumor necrosis factor alpha (TNF-alpha) in the two strains. TNF-alpha levels were significantly increased in the genital tract secretions of C57 mice compared to that of C3H mice during the first week of infection. Increased TNF-alpha may be beneficial to the host by leading to earlier eradication of infection, thereby preventing infection of the oviduct and thus the major disease sequelae associated with chlamydial infection of the genital tract.
This paper references
10.2307/3758354
Manual of clinical microbiology
P. Murray (1975)
10.1136/sti.55.6.422
Chlamydia trachomatis infection of the Fallopian tubes. Histological findings in two patients.
B. Møller (1979)
Chlamydiae (psittacosis-lymphogranuloma venereumtrachoma group), p. 357–364
J. Schachter (1980)
Chlamydiae ( psittacosis - lymphogranuloma venereum - trachoma group )
E. H. Lennette (1980)
10.1128/iai.31.3.1161-1176.1981
Purification and partial characterization of the major outer membrane protein of Chlamydia trachomatis
H. Caldwell (1981)
Salpingitis in mice induced by human strains of Chlamydia trachomatis.
M. Tuffrey (1986)
10.1016/0008-8749(86)90225-X
The in vitro bactericidal activity of peritoneal and spleen cells from Listeria-resistant and -susceptible mouse strains.
P. Wood (1986)
Genetic control of responsiveness of mice to Chlamydia trachomatis pneumonitis
D. M. Graham (1986)
Genetic control of responsiveness of mice to Chlamydia trachomatis pneumonitis , p . 396
T. Triglia (1986)
10.1097/00007435-198707000-00008
Effect of Prior Sexually Transmitted Disease on the Isolation of Chlamydia trachomatis
B. Katz (1987)
10.1001/JAMA.1987.03390150086041
Chlamydia trachomatis infections in the United States. What are they costing us?
A. Washington (1987)
10.1128/iai.58.6.2021-2023.1990
Variation in virulence among oculogenital serovars of Chlamydia trachomatis in experimental genital tract infection
J. Ito (1990)
10.1128/iai.58.6.1572-1576.1990
A role in vivo for tumor necrosis factor alpha in host defense against Chlamydia trachomatis
D. Williams (1990)
Genetic control of natural resistance to Chlamydia psittaci loth strain in mice
B Fuentes (1990)
Genetic control of natural resistance to Chlamydia psittaci loth strain in mice, p. 232
B. Fuentes (1990)
10.1016/0002-9378(91)90559-A
Genital chlamydial infections: epidemiology and reproductive sequelae.
W. Cates (1991)
10.1128/iai.59.7.2370-2375.1991
Production of colony-stimulating factors during pneumonia caused by Chlamydia trachomatis
D. Magee (1991)
10.1099/00221287-138-8-1707
Heterotypic protection of mice against chlamydial salpingitis and colonization of the lower genital tract with a human serovar F isolate of Chlamydia trachomatis by prior immunization with recombinant serovar L1 major outer-membrane protein.
M. Tuffrey (1992)
10.1128/iai.60.3.1217-1220.1992
Chlamydia trachomatis pneumonia induces in vivo production of interleukin-1 and -6
D. Magee (1992)
10.1128/iai.62.5.2094-2097.1994
Intravaginal inoculation of mice with the Chlamydia trachomatis mouse pneumonitis biovar results in infertility
L. M. de la Maza (1994)
10.1128/iai.63.5.1784-1789.1995
Local Th1-like responses are induced by intravaginal infection of mice with the mouse pneumonitis biovar of Chlamydia trachomatis
T. Cain (1995)
10.1128/iai.63.12.4675-4681.1995
Tumor necrosis factor alpha activity in genital tract secretions of guinea pigs infected with chlamydiae
T. Darville (1995)
Mutational analysis of TNF-alpha gene reveals a regulatory role for the 3'-untranslated region in the genetic predisposition to lupus-like autoimmune disease.
C. Jacob (1996)
10.1128/iai.64.12.4976-4983.1996
Initial route of antigen administration alters the T-cell cytokine profile produced in response to the mouse pneumonitis biovar of Chlamydia trachomatis following genital infection
K. Kelly (1996)
Genetically determined differences in IL-10 and IFN-gamma responses correlate with clearance of Chlamydia trachomatis mouse pneumonitis infection.
X. Yang (1996)
10.1172/JCI119136
Secretion of proinflammatory cytokines by epithelial cells in response to Chlamydia infection suggests a central role for epithelial cells in chlamydial pathogenesis.
S. Rasmussen (1997)



This paper is referenced by
10.1038/s41541-021-00312-9
A Chlamydia trachomatis VD1-MOMP vaccine elicits cross-neutralizing and protective antibodies against C/C-related complex serovars
A. Olsen (2021)
10.1128/IAI.00841-19
Fluorescence-Reported Allelic Exchange Mutagenesis-Mediated Gene Deletion Indicates a Requirement for Chlamydia trachomatis Tarp during In Vivo Infectivity and Reveals a Specific Role for the C Terminus during Cellular Invasion
Susmita Ghosh (2020)
10.1128/IAI.00413-20
Chlamydia-Specific IgA Secretion in the Female Reproductive Tract Induced via Per-Oral Immunization Confers Protection against Primary Chlamydia Challenge
Nita Shillova (2020)
10.17352/jvi.000030
Intranasal vaccination with a Chimeric Chlamydial Antigen BD584 confers protection against Chlamydia trachomatis genital tract infection
Liang Steven (2020)
10.1038/s41598-020-72409-5
Shift work influences the outcomes of Chlamydia infection and pathogenesis
Stephanie R Lundy (2020)
10.1080/21645515.2020.1717183
Protection of outbred mice against a vaginal challenge by a Chlamydia trachomatis serovar E recombinant major outer membrane protein vaccine is dependent on phosphate substitution in the adjuvant
S. Pal (2020)
10.1016/b978-0-12-811924-2.00036-5
Sexually Transmitted Infections and the Urgent Need for Vaccines: A Review of Four Major Bacterial STI Pathogens
A. Kollipara (2020)
10.4236/WJV.2019.92004
Evaluating Potential Vaccine Antigens in both the Chlamydia trachomatis and Chlamydia muridarum Intravaginal Mouse Challenge Models
R. Kaufhold (2019)
10.1371/journal.pone.0207422
TLR3 deficiency exacerbates the loss of epithelial barrier function during genital tract Chlamydia muridarum infection
R. Kumar (2019)
Effect of Chlamydia trachomatis infection and subsequent TNFa secretion on apoptosis in the murine genital tract
J. Perfettini (2019)
10.1128/IAI.00143-18
T Cell-Independent Gamma Interferon and B Cells Cooperate To Prevent Mortality Associated with Disseminated Chlamydia muridarum Genital Tract Infection
Taylor B Poston (2018)
10.1007/978-3-319-71232-1
Biology of Chlamydia
G. Häcker (2018)
10.1002/9781119380924.CH8
Chlamydia trachomatis urogenital infections: Epidemiology, clinical presentations, and pathogenesis
C. W. Armitage (2018)
10.1371/journal.pone.0195165
Toll-like receptor 3 (TLR3) promotes the resolution of Chlamydia muridarum genital tract infection in congenic C57BL/6N mice
S. Carrasco (2018)
10.1101/459636
TLR3 deficiency exacerbates the loss of epithelial barrier function during genital tract Chlamydia muridarum infection
R. Kumar (2018)
10.1007/82_2016_6
Chlamydia trachomatis: Protective Adaptive Responses and Prospects for a Vaccine.
Taylor B Poston (2018)
10.1016/j.ebiom.2018.02.020
Pelvic Chlamydial Infection Predisposes to Ectopic Pregnancy by Upregulating Integrin β1 to Promote Embryo-tubal Attachment
S. F. Ahmad (2018)
10.1071/RD17241
Effect of lipopolysaccharide on the expression of inflammatory mRNAs and microRNAs in the mouse oviduct.
K. L. Cerny (2018)
10.1016/j.micinf.2018.02.007
Activation of neutrophils by Chlamydia trachomatis-infected epithelial cells is modulated by the chlamydial plasmid.
S. Lehr (2018)
10.1128/IAI.00640-17
Fluorescence-Reported Allelic Exchange Mutagenesis Reveals a Role for Chlamydia trachomatis TmeA in Invasion That Is Independent of Host AHNAK
M. J. McKuen (2017)
10.1128/CVI.00543-16
Update on Chlamydia trachomatis Vaccinology
L. M. de la Maza (2017)
10.1038/s41598-017-09193-2
Genome-wide profiling of humoral immunity and pathogen genes under selection identifies immune evasion tactics of Chlamydia trachomatis during ocular infection
H. Pickering (2017)
10.1093/femspd/ftx045
Effect of cold water‐induced stress on immune response, pathology and fertility in mice during Chlamydia muridarum genital infection
T. Belay (2017)
10.3390/vaccines5010003
A Protective Vaccine against Chlamydia Genital Infection Using Vault Nanoparticles without an Added Adjuvant
Janina Jiang (2017)
10.1128/IAI.00046-17
Chlamydia trachomatis Cellular Exit Alters Interactions with Host Dendritic Cells
Ashley M. Sherrid (2017)
10.1128/CVI.00010-17
The Predominant CD4+ Th1 Cytokine Elicited to Chlamydia trachomatis Infection in Women Is Tumor Necrosis Factor Alpha and Not Interferon Gamma
S. J. Jordan (2017)
10.4049/jimmunol.1601314
CCR7 Deficiency Allows Accelerated Clearance of Chlamydia from the Female Reproductive Tract
L. Li (2017)
10.1371/journal.pone.0178537
Rectal administration of a chlamydial subunit vaccine protects against genital infection and upper reproductive tract pathology in mice
R. Pais (2017)
10.17037/PUBS.03928322
Identification of Chlamydia trachomatis immune targets through immunological and population-genomic screens and elucidation of potential roles in bacterial pathogenesis
H. Pickering (2017)
10.4049/jimmunol.1700914
A Chlamydia-Specific TCR-Transgenic Mouse Demonstrates Th1 Polyfunctionality with Enhanced Effector Function
Taylor B Poston (2017)
10.1016/j.tim.2016.05.007
Chlamydia trachomatis Genital Tract Infections: When Host Immune Response and the Microbiome Collide.
Noa Ziklo (2016)
The role of regulatory T and B cells in host responses to Chlamydia genital tract infection
J. Moore-Connors (2016)
See more
Semantic Scholar Logo Some data provided by SemanticScholar