← Back to Search
Transcutaneous Immunization With Combined Cholera Toxin And CpG Adjuvant Protects Against Chlamydia Muridarum Genital Tract Infection
L. J. Berry, D. Hickey, K. Skelding, S. Bao, Amanda M Rendina, P. Hansbro, C. M. Gockel, K. Beagley
Published 2004 · Biology, Medicine
Save to my Library
Download via 🐼 PaperPanda Download via oaDOI Download via OAB Download via LibKey Download via Google Google ScholarAnalyze on Scholarcy Visualize in Litmaps
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
ABSTRACT Chlamydia trachomatis is a pathogen of the genital tract and ocular epithelium. Infection is established by the binding of the metabolically inert elementary body (EB) to epithelial cells. These are taken up by endocytosis into a membrane-bound vesicle termed an inclusion. The inclusion avoids fusion with host lysosomes, and the EBs differentiate into the metabolically active reticulate body (RB), which replicates by binary fission within the protected environment of the inclusion. During the extracellular EB stage of the C. trachomatis life cycle, antibody present in genital tract or ocular secretions can inhibit infection both in vivo and in tissue culture. The RB, residing within the intracellular inclusion, is not accessible to antibody, and resolution of infection at this stage requires a cell-mediated immune response mediated by gamma interferon-secreting Th1 cells. Thus, an ideal vaccine to protect against C. trachomatis genital tract infection should induce both antibody (immunoglobulin A [IgA] and IgG) responses in mucosal secretions to prevent infection by chlamydial EB and a strong Th1 response to limit ascending infection to the uterus and fallopian tubes. In the present study we show that transcutaneous immunization with major outer membrane protein (MOMP) in combination with both cholera toxin and CpG oligodeoxynucleotides elicits MOMP-specific IgG and IgA in vaginal and uterine lavage fluid, MOMP-specific IgG in serum, and gamma interferon-secreting T cells in reproductive tract-draining caudal and lumbar lymph nodes. This immunization protocol resulted in enhanced clearance of C. muridarum (C. trachomatis, mouse pneumonitis strain) following intravaginal challenge of BALB/c mice.
This paper references
Definitions of infectious and infectious-like conditions in the lower genital tract of the female.
L. Weström (1983)
Effect of antithymocyte serum on the course of chlamydial genital infection in female guinea pigs
R. Rank (1983)
Correlation of host immune response with quantitative recovery of Chlamydia trachomatis from the human endocervix
R. Brunham (1983)
Estradiol regulation of secretory component in the uterus of the rat: evidence for involvement of RNA synthesis.
C. Wira (1984)
Role of tryptophan degradation in respiratory burst-independent antimicrobial activity of gamma interferon-stimulated human macrophages
H. Murray (1989)
Role of tryptophan degraVOL
H. W. Murray (1989)
Intracellular neutralization of virus by immunoglobulin A antibodies.
M. Mazanec (1992)
A three-tiered view of the role of IgA in mucosal defense.
M. Mazanec (1993)
Partial protection against genital reinfection by immunization of guinea-pigs with isolated outer-membrane proteins of the chlamydial agent of guinea-pig inclusion conjunctivitis.
B. Batteiger (1993)
Protective cytotoxic T lymphocytes are induced during murine infection with Chlamydia trachomatis.
M. Starnbach (1994)
Animal models for urogenital infections.
R. Rank (1994)
Protective efficacy of major outer membrane protein-specific immunoglobulin A (IgA) and IgG monoclonal antibodies in a murine model of Chlamydia trachomatis genital tract infection
T. W. Cotter (1995)
Murine cytotoxic T lymphocytes induced following Chlamydia trachomatis intraperitoneal or genital tract infection respond to cells infected with multiple serovars
M. Starnbach (1995)
Neutrophils from human immunodeficiency virus (HIV)-seronegative donors induce HIV replication from HIV-infected patients' mononuclear cells and cell lines: an in vitro model of HIV transmission facilitated by Chlamydia trachomatis
J. Ho (1995)
Gene knockout mice establish a primary protective role for major histocompatibility complex class II-restricted responses in Chlamydia trachomatis genital tract infection
R. Morrison (1995)
4 – Cholera Toxin as a Mucosal Adjuvant
C. Elson (1996)
Protective Effect of Rotavirus VP6-Specific IgA Monoclonal Antibodies That Lack Neutralizing Activity
John W. Burns (1996)
A recombinant Chlamydia trachomatis major outer membrane protein binds to heparan sulfate receptors on epithelial cells.
H. Su (1996)
Nitric oxide production: a mechanism of Chlamydia trachomatis inhibition in interferon-γ-treated RAW264.7 cells
B. Chen (1996)
Nitric oxide production: a mechanism of Chlamydia trachomatis inhibition in interferon-gammatreated RAW264.7 cells
B. Chen (1996)
Mucosal vaccines, p. 59–72
C. O. Elson (1996)
Immunity to Chlamydia trachomatis is mediated by T helper 1 cells through IFN-gamma-dependent and -independent pathways.
L. Perry (1997)
Genital tract infection with Chlamydia trachomatis fails to induce protective immunity in gamma interferon receptor-deficient mice despite a strong local immunoglobulin A response
M. Johansson (1997)
Dissemination of Chlamydia trachomatis chronic genital tract infection in gamma interferon gene knockout mice
T. W. Cotter (1997)
The Hidden Epidemic: Confronting Sexually Transmitted Diseases
T. Eng (1997)
Mucosal immunity in the human female reproductive tract: cytotoxic T lymphocyte function in the cervix and vagina of premenopausal and postmenopausal women.
H. D. White (1997)
Chlamydia trachomatis genital tract infection of antibody-deficient gene knockout mice
H. Su (1997)
A nontoxic mutant of cholera toxin elicits Th2-type responses for enhanced mucosal immunity.
S. Yamamoto (1997)
CpG Oligodeoxynucleotides Act as Adjuvants that Switch on T Helper 1 (Th1) Immunity
R. Chu (1997)
Mutants in the ADP-ribosyltransferase Cleft of Cholera Toxin Lack Diarrheagenicity but Retain Adjuvanticity
Shingo Yamamoto (1997)
Synthetic peptides based on Chlamydia trachomatis antigens identify cytotoxic T lymphocyte responses in subjects from a trachoma‐endemic population
M. Holland (1997)
Virus-specific IgA reduces hepatic viral titers in vivo on mouse hepatitis (MHV) infection
D. S. Huang (1997)
Activation of cutaneous dendritic cells by CpG-containing oligodeoxynucleotides: a role for dendritic cells in the augmentation of Th1 responses by immunostimulatory DNA.
T. Jakob (1998)
A nontoxic adjuvant for mucosal immunity to pneumococcal surface protein A.
M. Yamamoto (1998)
Sexually transmitted diseases and reproductive health in women.
H. V. Burst (1998)
Immune Control of Chlamydial Growth in the Human Epithelial Cell Line RT4 Involves Multiple Mechanisms That Include Nitric Oxide Induction, Tryptophan Catabolism and Iron Deprivation
J. Igietseme (1998)
Inhibition of Apoptosis in Chlamydia-infected Cells: Blockade of Mitochondrial Cytochrome c Release and Caspase Activation
Tao Fan (1998)
Skin immunization made possible by cholera toxin
G. Glenn (1998)
Transcutaneous Immunization with Bacterial ADP-Ribosylating Exotoxins as Antigens and Adjuvants
G. Glenn (1999)
Principles of transcutaneous immunization using cholera toxin as an adjuvant.
T. Scharton‐Kersten (1999)
Chlamydia trachomatis infections: progress and problems.
W. Stamm (1999)
Induction of HLA class I-restricted CD8+ CTLs specific for the major outer membrane protein of Chlamydia trachomatis in human genital tract infections.
S. K. Kim (1999)
DNA activates human immune cells through a CpG sequence‐dependent manner
M. Bauer (1999)
Clearance of Chlamydia trachomatis from the Murine Genital Mucosa Does Not Require Perforin-Mediated Cytolysis or Fas-Mediated Apoptosis
L. Perry (1999)
Mucosal immunity in the human female reproductive tract, p. 1423–1434
W. H. Kutteh (1999)
Mucosal adjuvants, p. 817–838
C. O. Elson (1999)
Degradation of Transcription Factor Rfx5 during the Inhibition of Both Constitutive and Interferon γ–Inducible Major Histocompatibility Complex Class I Expression in Chlamydia-Infected Cells
G. Zhong (2000)
Multiple drug-resistant Chlamydia trachomatis associated with clinical treatment failure.
J. Somani (2000)
Delineation of a CpG Phosphorothioate Oligodeoxynucleotide for Activating Primate Immune Responses In Vitro and In Vivo1
G. Hartmann (2000)
Direct Detection and Magnetic Isolation of Chlamydia trachomatis Major Outer Membrane Protein-Specific CD8+ CTLs with HLA Class I Tetramers1
S. K. Kim (2000)
Cutting Edge: The Mucosal Adjuvant Cholera Toxin Redirects Vaccine Proteins into Olfactory Tissues
F. van Ginkel (2000)
Transcutaneous immunization induces mucosal and systemic immunity: a potent method for targeting immunity to the female reproductive tract.
C. M. Gockel (2000)
Immunity to Murine Chlamydia trachomatis Genital Tract Reinfection Involves B Cells and CD4+ T Cells but Not CD8+ T Cells
S. Morrison (2000)
Chlamydia trachomatis infection: incidence, health costs and prospects for vaccine development.
K. Beagley (2000)
Oral, intrarectal and intranasal immunizations using CpG and non-CpG oligodeoxynucleotides as adjuvants.
M. McCluskie (2000)
GLOBAL PREVALENCE AND INCIDENCE OF SELECTED CURABLE SEXUALLY TRANSMITTED DISEASES: OVERVIEW AND ESTIMATES
A Toll-like receptor recognizes bacterial DNA
H. Hemmi (2000)
Direct detection and magnetic isolation of Chlamydia trachomatis major outer membrane protein-specific CD8ϩ CTLs with HLA class I tetramers
S K Kim (2000)
Direct detection and magnetic isolation of Chlamydia trachomatis major outer membrane protein-specific CD8 CTLs with HLA class I tetramers
S. K. Kim (2000)
Real-time polymerase chain reaction analysis reveals an evolution of cytokine mRNA production in allograft acceptor mice.
D. Xia (2001)
CpG ODN can re-direct the Th bias of established Th2 immune responses in adult and young mice.
R. Weeratna (2001)
The use of CpG DNA as a mucosal vaccine adjuvant.
M. McCluskie (2001)
The potential of CpG oligodeoxynucleotides as mucosal adjuvants.
M. McCluskie (2001)
CpG motif identification for veterinary and laboratory species demonstrates that sequence recognition is highly conserved.
R. Rankin (2001)
CpG motifs in bacterial DNA and their immune effects.
A. Krieg (2002)
The intracellular life of chlamydiae.
M. Hammerschlag (2002)
From A to Z on CpG.
A. Krieg (2002)
CpG-oligodinucleotides as an effective adjuvant in pigs for intramuscular immunizations.
Y. van der Stede (2002)
Chlamydia vaccines: strategies and status.
J. Igietseme (2002)
Immunity to Murine Chlamydial Genital Infection
R. Morrison (2002)
Highly immunostimulatory CpG-free oligodeoxynucleotides for activation of human leukocytes.
J. Vollmer (2002)
Multiple Chlamydia pneumoniae Antigens Prime CD8+ Tc1 Responses That Inhibit Intracellular Growth of This Vacuolar Pathogen1
B. Wizel (2002)
Fc receptor regulation of protective immunity against Chlamydia trachomatis
T. Moore (2002)
A Chlamydia trachomatis-Specific Th2 Clone Does Not Provide Protection against a Genital Infection and Displays Reduced Trafficking to the Infected Genital Mucosa
R. Hawkins (2002)
A nontoxic chimeric enterotoxin adjuvant induces protective immunity in both mucosal and systemic compartments with reduced IgE antibodies.
M. Kweon (2002)
A dilemma for mucosal vaccination: efficacy versus toxicity using enterotoxin-based adjuvants.
K. Fujihashi (2002)
T cell responses to Chlamydia trachomatis.
W. P. Loomis (2002)
Proteins in the chlamydial inclusion membrane.
D. Rockey (2002)
Infection dose as a critical factor in determining treatment effect in an animal model of chlamydial genital infection
R G Rank (2002)
Infection dose as a critical factor in determining treatment effect in an animal model of chlamydial genital infection, p. 253–256
R. G. Rank (2002)
The lineage decisions of helper T cells
K. M. Murphy (2002)
L. Stevceva (2005)
This paper is referenced by
Chlamydia trachomatis vaccines for genital infections: where are we and how far is there to go?
L. M. de la Maza (2021)
A proposed roadmap for the control of infections in wildlife using Chlamydia vaccine development in koalas Phascolarctos cinereus as a template
C. Waugh (2020)
Regulation of Mucosal Immunity in the Genital Tract: Balancing Reproduction and Protective Immunity
D. Hickey (2020)
CCR10 expression is required for the adjuvant activity of the mucosal chemokine CCL28 when delivered in the context of an HIV-1 Env DNA vaccine.
E. N. Gary (2020)
Chlamydia muridarum infection differentially alters smooth muscle function in mouse uterine horn and cervix.
Jia-Ming Lee (2020)
Immunology-Guided Biomaterial's Design as Mucosal Cancer Vaccine.
Shiran Ferber (2019)
Enhancing humoral immunity via sustained-release implantable microneedle patch vaccination
Archana V. Boopathy (2019)
Chlamydia‐infected macrophages are resistant to azithromycin treatment and are associated with chronic oviduct inflammation and hydrosalpinx development
M. Harvie (2019)
Seventy Years of Chlamydia Vaccine Research – Limitations of the Past and Directions for the Future
Samuel L Phillips (2019)
Chlamydia trachomatis urogenital infections: Epidemiology, clinical presentations, and pathogenesis
C. W. Armitage (2018)
Chlamydia muridarum infection differentially changes smooth muscle contractility and responses to prostaglandins in uterus and cervix
J. Lee (2018)
Topical CpG Oligodeoxynucleotide Adjuvant Enhances the Adaptive Immune Response against Influenza A Infections
Wing Ki Cheng (2016)
Induction of mucosal immunity through systemic immunization: Phantom or reality?
Fei Su (2016)
Chlamydia muridarum Infection-Induced Destruction of Male Germ Cells and Sertoli Cells Is Partially Prevented by Chlamydia Major Outer Membrane Protein-Specific Immune CD4 cells1
A. Sobinoff (2015)
Comparison of subcutaneous versus intranasal immunization of male koalas (Phascolarctos cinereus) for induction of mucosal and systemic immunity against Chlamydia pecorum.
C. Waugh (2015)
Animal models of immunity to female genital tract infections and vaccine development
C. Kaushic (2015)
Immunization with Heat Shock Protein A and γ-Glutamyl Transpeptidase Induces Reduction on the Helicobacter pylori Colonization in Mice
X. Zhang (2015)
Chlamydial disease and other environmental pressures affecting an opportunistically sampled wild koala population in the Port Stephens region
D. Hudson (2015)
Hepatitis B virus core antigen as a carrier for Chlamydia trachomatis MOMP multi-epitope peptide enhances protection against genital chlamydial infection
Pengfei Jiang (2015)
Immune Regulation of Chlamydia trachomatis Infections of the Female Genital Tract
L. Hafner (2014)
Mécanismes impliqués dans la polarisation des lymphocytes T CD4+ folliculaires et l'initiation de l'immunité muqueuse après immunisation intradermique par un antigène particulaire
C. Nuttens (2014)
CTA 1-DD is an effective adjuvant for targeting anti-chlamydial immunity to the genital mucosa
K. Cunningham (2014)
Immunogenicity of a West Nile Virus DIII-Cholera Toxin A2/B Chimera after Intranasal Delivery
J. Tinker (2014)
A peptide-based nanofibrous hydrogel as a promising DNA nanovector for optimizing the efficacy of HIV vaccine.
Y. Tian (2014)
Toxinas termo-lábeis (LTs) do tipo II de Escherichia coli enterotoxigênica (ETEC): efeito adjuvante e atividade inflamatória
Camila Mathias dos Santos (2014)
Immunity against a Chlamydia infection and disease may be determined by a balance of IL‐17 signaling
C. O'Meara (2014)
Immunity, immunopathology, and human vaccine development against sexually transmitted Chlamydia trachomatis
J. Rey-Ladino (2014)
Hepatitis B virus surface antigen as delivery vector can enhance Chlamydia trachomatis MOMP multi-epitope immune response in mice
Shanli Zhu (2014)
Chlamydia trachomatis recombinant MOMP encapsulated in PLGA nanoparticles triggers primarily T helper 1 cellular and antibody immune responses in mice: a desirable candidate nanovaccine
Stacie J. Fairley (2013)
Proteolytic activation of Chlamydia trachomatis HTRA is mediated by PDZ1 domain interactions with protease domain loops L3 and LC and beta strand β5
J. W. Marsh (2013)
Antigenic specificity of a monovalent versus polyvalent MOMP based Chlamydia pecorum vaccine in koalas (Phascolarctos cinereus).
A. Kollipara (2013)
Immunotherapeutic potential of CpG oligodeoxynucleotides in veterinary species
A. Manuja (2013)See more