Online citations, reference lists, and bibliographies.
← Back to Search

The Chromate-inducible ChrBACF Operon From The Transposable Element TnOtChr Confers Resistance To Chromium(VI) And Superoxide.

R. Branco, A. P. Chung, T. Johnston, Volkan Gurel, P. Morais, A. Zhitkovich
Published 2008 · Medicine, Biology

Cite This
Download PDF
Analyze on Scholarcy
Large-scale industrial use of chromium(VI) has resulted in widespread contamination with carcinogenic chromium(VI). The abilities of microorganisms to survive in these environments and to detoxify chromate require the presence of specific resistance systems. Here we report identification of the transposon-located (TnOtChr) chromate resistance genes from the highly tolerant strain Ochrobactrum tritici 5bvl1 surviving chromate concentrations of >50 mM. The 7,189-bp-long TnOtChr of the mixed Tn21/Tn3 transposon subfamily contains a group of chrB, chrA, chrC, and chrF genes situated between divergently transcribed resolvase and transposase genes. The chrB and chrA genes, but not chrF or chrC, were essential for establishment of high resistance in chromium-sensitive O. tritici. The chr promoter was strongly induced by chromate or dichromate, but it was completely unresponsive to Cr(III), oxidants, sulfate, or other oxyanions. Plasmid reporter experiments identified ChrB as a chromate-sensing regulator of chr expression. Induction of the chr operon suppressed accumulation of cellular Cr through the activity of a chromate efflux pump encoded by chrA. Expression of chrB, chrC, or chrF in an Escherichia coli sodA sodB double mutant restored its aerobic growth in minimal medium and conferred resistance to superoxide-generating agents menadione and paraquat. Nitroblue tetrazolium staining on native gels showed that ChrC protein had superoxide dismutase activity. TnOtChr appears to represent a mobile genetic system for the distribution of the chromate-regulated resistance operon. The presence of three genes protecting against superoxide toxicity should provide an additional survival advantage to TnOtChr-containing cells in the environments with multiple redox-active contaminants.
This paper references
A Short Course in Bacterial Genetics: A Laboratory Manual and Handbook for Escherichia coli and Rela
J. Miller (1992)
Human Nucleotide Excision Repair Efficiently Removes Chromium-DNA Phosphate Adducts and Protects Cells against Chromate Toxicity*
Mindy Reynolds (2004)
Interactions of chromium with microorganisms and plants.
C. Cervantes (2001)
Tn5563, a transposon encoding putative mercuric ion transport proteins located on plasmid pRA2 of Pseudomonas alcaligenes.
C. C. Yeo (1998)
Carcinogenic chromium(VI) induces cross-linking of vitamin C to DNA in vitro and in human lung A549 cells.
G. Quievryn (2002)
Causes of DNA single-strand breaks during reduction of chromate by glutathione in vitro and in cells.
J. Messer (2006)
The bzd gene cluster, coding for anaerobic benzoate catabolism, in Azoarcus sp. strain CIB.
M. J. López Barragán (2004)
Gapped BLAST and PSI-BLAST: A new
D. Lipman (1997)
Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes.
M. Kovach (1995)
Analysis and construction of stable phenotypes in gram-negative bacteria with Tn5- and Tn10-derived minitransposons.
V. de Lorenzo (1994)
Decreased chromate uptake in Pseudomonas fluorescens carrying a chromate resistance plasmid.
H. Ohtake (1987)
Nucleotide Sequence of Plasmid pCNB1 from Comamonas Strain CNB-1 Reveals Novel Genetic Organization and Evolution for 4-Chloronitrobenzene Degradation
Y. Ma (2007)
Cloning and expression of plasmid genes encoding resistances to chromate and cobalt in Alcaligenes eutrophus.
A. Nies (1989)
Diversity of chromium‐resistant and ‐reducing bacteria in a chromium‐contaminated activated sludge
R. Francisco (2002)
Effect of chromate stress on Escherichia coli K-12.
D. Ackerley (2006)
A short course in bacterial genetics
J. Miller (1992)
Chromium: exposure, toxicity, and biomonitoring approaches
A. Zhitkovich (2002)
Biomarkers of Environmentally Associated Disease: Technologies, Concepts, and Perspectives
S. Wilson (2002)
New genes involved in chromate resistance in Ralstonia metallidurans strain CH34
S. Juhnke (2004)
Alcaligenes eutrophus CH34 is a facultative chemolithotroph with plasmid-bound resistance to heavy metals.
M. Mergeay (1985)
Transcriptional regulation of the mercury-resistance genes of transposon Tn501.
P. Lund (1986)
Importance of chromium-DNA adducts in mutagenicity and toxicity of chromium(VI).
A. Zhitkovich (2005)
CHR, a novel family of prokaryotic proton motive force-driven transporters probably containing chromate/sulfate antiporters.
D. Nies (1998)
Alcaligenes eutrophus as a Bacterial Chromate Sensor
Nicola Peitzsch (1998)
Molecular cloning: A laboratory manual 2nd ed. Edited by J. . Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989 (in 3 volumes)
P. Deininger (1990)
Why Superoxide Imposes an Aromatic Amino Acid Auxotrophy onEscherichia coli
L. Benov (1999)
Nucleotide sequence and expression of a plasmid-encoded chromate resistance determinant from Alcaligenes eutrophus.
A. Nies (1990)
The Tn21 subgroup of bacterial transposable elements.
J. Grinsted (1990)
Plasmid-determined resistance to chromate in Pseudomonas aeruginosa
C. Cervantes (1988)
Suppression of oxidative envelope damage by pseudoreversion of a superoxide dismutase-deficient mutant of Escherichia coli.
J. Imlay (1992)
Nei deficient Escherichia coli are sensitive to chromate and accumulate the oxidized guanine lesion spiroiminodihydantoin.
M. Hailer (2005)
Transposon Tn21, Flagship of the Floating Genome
C. Liebert (1999)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
Genotoxicity and mutagenicity of chromium(VI)/ascorbate-generated DNA adducts in human and bacterial cells.
G. Quievryn (2003)
The 79,370-bp conjugative plasmid pB4 consists of an IncP-1β backbone loaded with a chromate resistance transposon, the strA-strB streptomycin resistance gene pair, the oxacillinase gene blaNPS-1, and a tripartite antibiotic efflux system of the resistance-nodulation-division family
A. Tauch (2003)
An unusual Tn21-like transposon containing an ars operon is present in highly arsenic-resistant strains of the biomining bacterium Acidithiobacillus caldus.
I. M. Tuffin (2005)
Cloning, nucleotide sequence, and expression of the chromate resistance determinant of Pseudomonas aeruginosa plasmid pUM505.
C. Cervantes (1990)
Efflux of chromate by Pseudomonas aeruginosa cells expressing the ChrA protein.
Betzabe E Pimentel (2002)
Isolation, characterization and expression of a plasmid encoding chromate resistance in Pseudomonas putida KT2441
Mondaca (1998)
Inorganic salts resistance associated with a lactose-fermenting plasmid in Streptococcus lactis.
J. Efstathiou (1977)
Resistance Determinants of a Highly Arsenic-Resistant Strain of Leptospirillum ferriphilum Isolated from a Commercial Biooxidation Tank
I. M. Tuffin (2006)
Superoxide dismutase: improved assays and an assay applicable to acrylamide gels.
C. Beauchamp (1971)
Ochrobactrum tritici strain 5bvl1 - characterization of a Cr(VI)-resistant and Cr(VI)-reducing strain.
Rita Branco (2004)
Chromate efflux by means of the ChrA chromate resistance protein from Pseudomonas aeruginosa.
A. H. Álvarez (1999)
Chromate resistance plasmid in Pseudomonas fluorescens.
L. Bopp (1983)

This paper is referenced by
The ChrA homologue from a sulfur-regulated gene cluster in cyanobacterial plasmid pANL confers chromate resistance
Esther Aguilar-Barajas (2012)
Characterization and genomic analysis of chromate resistant and reducing Bacillus cereus strain SJ1
Minyan He (2010)
Chromium in Drinking Water: Sources, Metabolism, and Cancer Risks
A. Zhitkovich (2011)
Damage control: regulating defenses against toxic metals and metalloids.
A. Summers (2009)
Recent bioreduction of hexavalent chromium in wastewater treatment: A review
D. Pradhan (2017)
Tn5045, a novel integron-containing antibiotic and chromate resistance transposon isolated from a permafrost bacterium.
M. Petrova (2011)
Analysis of the Genome and Chromium Metabolism-Related Genes of Serratia sp. S2
Lanlan Dong (2017)
Biogenèse de la pellicule chez Shewanella oneidensis
Cyril Gambari (2018)
Assessment of heavy metal tolerance and hexavalent chromium reducing potential of Corynebacterium paurometabolum SKPD 1204 isolated from chromite mine seepage
Satarupa Dey (2016)
Identification and Characterization of the Transcriptional Regulator ChrB in the Chromate Resistance Determinant of Ochrobactrum tritici 5bvl1
R. Branco (2013)
Natural Hot Spots for Gain of Multiple Resistances: Arsenic and Antibiotic Resistances in Heterotrophic, Aerobic Bacteria from Marine Hydrothermal Vent Fields
P. Farias (2015)
Class 1 integrons in benthic bacterial communities: abundance, association with Tn402-like transposition modules and evidence for coselection with heavy-metal resistance.
Carly P. Rosewarne (2010)
Whole-cell bioreporters for the detection of bioavailable metals.
A. Hynninen (2010)
Life in High Salt Concentrations with Changing Environmental Conditions: Insights from Genomic and Phenotypic Analysis of Salinivibrio sp.
Jojy John (2019)
Reduction of Cr(VI) by Bacillus species isolated from tannery effluent contaminated sites of Tamil Nadu, India
S. Princy (2020)
Multi-omics response of Pannonibacter phragmitetus BB to hexavalent chromium.
Li-yuan Chai (2019)
Highly Sensitive, Highly Specific Whole-Cell Bioreporters for the Detection of Chromate in Environmental Samples
R. Branco (2013)
Revealing the biotechnological potential of Delftia sp. JD2 by a genomic approach
M. Morel (2016)
Adsorption on activated carbon derived from tannery fleshing waste: Adsorption isotherms, thermodynamics, and kinetics
Yuvaraj Palani (2017)
Chromate-resistance genes in plasmids from antibiotic-resistant nosocomial enterobacterial isolates.
Gustavo G Caballero-Flores (2012)
Bacterial chromate reduction: A review of important genomic, proteomic, and bioinformatic analysis
S. K. Pradhan (2016)
Chromium resistance genetic element flanked by XerC/XerD recombination sites and its distribution in environmental and clinical Acinetobacter strains
S. Mindlin (2018)
Bacterial transport of sulfate, molybdate, and related oxyanions
Esther Aguilar-Barajas (2011)
Chromate tolerance and removal of bacterial strains isolated from uncontaminated and chromium-polluted environments
Dragana Tamindžija (2019)
Metagenomic analysis of river microbial communities
Thea Van Rossum (2017)
Multidrug resistance operon emrAB contributes for chromate and ampicillin co-resistance in a Staphylococcus strain isolated from refinery polluted river bank
H. Zhang (2016)
Impact of plant-associated bacteria biosensors on plant growth in the presence of hexavalent chromium
R. Francisco (2017)
Genetic basis and importance of metal resistant genes in bacteria for bioremediation of contaminated environments with toxic metal pollutants
S. Das (2016)
Environmental sensing of heavy metals through whole cell microbial biosensors: a synthetic biology approach.
Lara Bereza-Malcolm (2015)
Role of Bacterial Chromate Reductase in Bioremediation of Chromium-Containing Wastes
Satarupa Dey (2021)
Characterization and genomic analysis of a highly chromate resistant and reducing bacterial strain Lysinibacillus fusiformis ZC1.
Minyan He (2011)
Nucleotide sequence of Pseudomonas aeruginosa conjugative plasmid pUM505 containing virulence and heavy-metal resistance genes.
M. I. Ramírez-Díaz (2011)
See more
Semantic Scholar Logo Some data provided by SemanticScholar