Online citations, reference lists, and bibliographies.
Referencing for people who value simplicity, privacy, and speed.
Get Citationsy
← Back to Search

Synthesis Of Ribonucleic Acid And Protein In Plasmid-containing Minicells Of Escherichia Coli K-12.

K. J. Roozen, R. Fenwick, R. Curtiss
Published 1971 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
Unlike the deoxyribonucleic acid (DNA)-deficient minicells produced by F(-) parents, minicells produced by plasmid-containing strains contain significant amounts of plasmid DNA. We examined the ability of plasmid-containing minicells to synthesize ribonucleic acid (RNA) and protein. In vivo, minicells produced by F(-) parents are unable to incorporate radioactive precursors into acid-insoluble RNA or protein, whereas minicells produced by F', R(+), or Col(+) parents are capable of such synthesis. Using a variety of approaches, including polyacrylamide gel analysis of the RNA species produced and electron microscope autoradiography, we demonstrated that the synthesis observed in minicell preparations is a property of the plasmid-containing minicells and not a result of the few cells (approximately 1 per 10(6) minicells) contaminating the preparations. That the observed synthesis is of biological importance is suggested by the ability of plasmid-containing minicells to yield viable phage upon infection with T4.
This paper references
10.1128/JB.104.1.443-452.1970
Synthetic capabilities of plasmolyzed cells and spheroplasts of Escherichia coli.
K. Rubenstein (1970)
10.1073/PNAS.66.3.815
Segregation of functional sex factor into minicells.
L. Kass (1970)
Ultraviolet-induced genetic recombination in a partially diploid strain of Escherichia coli.
R. Curtiss (1968)
10.1016/B978-1-4832-2795-5.50017-4
HIGH-RESOLUTION AUTORADIOGRAPHY
L. Caro (1962)
10.1016/0042-6822(55)90016-7
Transduction of linked genetic characters of the host by bacteriophage P1.
E. Lennox (1955)
10.1038/2171184A0
Filamentous Phages specific for the I Sex Factor
G. Meynell (1968)
10.1016/S0079-6603(08)60315-3
Preparation, Fractionation, and Properties of sRNA
G. L. Brown (1963)
10.1128/JB.99.2.621-622.1969
Polyuridylic acid-directed phenylalanine incorporation in minicell extracts.
J. Fralick (1969)
10.1101/SQB.1968.033.01.071
The properties of DNA transferred to minicells during conjugation.
A. Cohen (1968)
Decryptifi - cation of RNA polymerase in whole cells in Escherichia coli
J Inselburg (1967)
10.1016/0005-2787(68)90396-1
RNA with amino acid-acceptor activity isolated from an oncogenic virus.
M. Travnicek (1968)
10.1016/0022-2836(68)90387-2
Small molecular weight monodisperse nuclear RNA.
R. Weinberg (1968)
10.1016/0022-2836(69)90368-4
Replication of a bacterial episome under relaxed control.
R. Rownd (1969)
10.1099/00221287-50-1-1
A proposal for a uniform nomenclature in bacterial genetics.
M. Demerec (1968)
10.1099/00221287-31-3-339
Penicillin-induced lysis in Escherichia coli.
H. G. Boman (1963)
10.1073/PNAS.67.3.1164
Detection of bacteriophage T4- and T5-coded transfer RNAs.
N. Scherberg (1970)
10.1016/0005-2787(68)90286-4
Mode of action of rafamycin on the RNA polymerase reaction.
A. Sippel (1968)
10.1101/SQB.1966.031.01.076
Studies on sRNA coded by herpes virus.
H. Subak-Sharpe (1966)
Progress in high - resolution autoradiography , p . 171 190
J. A. Butler (1966)
10.1038/1731223A0
Effect of Nucleic Acids on Protein Synthesis and Amino-Acid Incorporation in Disrupted Staphylococcal Cells
E. Gale (1954)
10.1073/PNAS.61.1.114
Transfer RNA coded by the T4 bacteriophage genome.
S. Weiss (1968)
10.1016/0003-2697(70)90297-6
Precision of RNA separation by polyacrylamide gel electrophoresis.
P. Lewicki (1970)
CHROMOSOMAL ABERRATIONS ASSOCIATED WITH MUTATIONS TO BACTERIOPHAGE RESISTANCE IN ESCHERICHIA COLI.
S. R. Curtis (1965)
10.1016/0022-2836(67)90206-9
Decryptification of RNA polymerase in whole cells of Escherichia coli.
F. Gros (1967)
10.1128/JB.104.1.34-44.1970
Circular R-factor molecules controlling penicillinase synthesis, replicating in Escherichia coli under either relaxed or stringent control.
P. Kontomichalou (1970)
10.1073/PNAS.57.2.321
MINIATURE escherichia coli CELLS DEFICIENT IN DNA.
H. Adler (1967)
10.1042/BJ0480298
The assimilation of amino-acids by bacteria; the action of inhibitors and antibiotics on the accumulation of free glutamic acid and the formation of combined of combined glutamate in Staphylococcus aureus.
E. Gale (1951)
10.1128/MMBR.32.4_PT_1.320-348.1968
Parental functions during conjugation in Escherichia coli K-12.
R. Curtiss (1968)



This paper is referenced by
10.1128/JB.146.2.552-563.1981
Role of ribonucleic acid synthesis in conjugational transfer of chromosomal and plasmid deoxyribonucleic acids.
L. Maturin (1981)
10.1007/BF00268058
Bacteriophage ϕ29 infection of Bacillus subtilis minicells
J. García (2004)
10.1016/0167-4781(84)90036-8
Cloning and expression of the genes for ribulose-1,5-bisphosphate carboxylase from Spirulina platensis
O. Tiboni (1984)
10.1016/0378-1119(81)90149-9
The cloning and analysis of the aroD gene of E. coli K-12.
J. Kinghorn (1981)
10.1038/270580A0
Conjugation proteins encoded by the F sex factor
N. Kennedy (1977)
10.1016/0378-1119(93)90544-D
Cloning and characterization of a gene coding for the catechol 1,2-dioxygenase of Arthrobacter sp. mA3.
R. Eck (1993)
10.1111/J.1574-6968.1985.TB01003.X
Preferential synthesis, localisation and solubilisation of a precursor form of Bacillus subtilis levansucrase in an Escherichia coli minicell system
F. Benyahia (1985)
10.1016/0003-2697(89)90253-4
Purification of plasmid-expressed proteins which lack functional assay systems.
C. C. Marvel (1989)
10.1385/1-59259-233-3:113
Producing soluble recombinant RNases and assays to measure their interaction with interferon-gamma in vitro.
C. Schein (2001)
10.1007/BF00275113
Genetic map of the bacteriocinogenic plasmid CLO DF13 derived by insertion of the transposon Tn901
P. Andreoli (2004)
10.1016/0022-2836(84)90346-2
Analysis of the trfA region of broad host-range plasmid RK2 by transposon mutagenesis and identification of polypeptide products.
V. Shingler (1984)
10.1099/00221287-129-5-1513
A cryptic plasmid from Shigella sonnei.
J. E. Croft (1983)
10.1016/B978-0-08-024417-4.50018-4
ENZYMATIC SYNTHESIS OF SITE-SPECIFIC MUTATIONS IN AN ESSENTIAL GENE OF BACTERIOPHAGE fX174
R. Chambers (1980)
10.1017/S001667230001911X
The phasmid as a tool for plasmid genetics: I. Fine structure of the β-lactamase gene
L. Castagnoli (1982)
10.1073/pnas.1501064112
Structural remodeling of bacteriophage T4 and host membranes during infection initiation
B. Hu (2015)
10.1126/SCIENCE.217087
Molecular cloning of polyoma virus DNA in Escherichia coli: plasmid vector system.
M. Israel (1979)
10.1016/0378-1119(78)90021-5
Transcription and translation in E. coli of hybrid plasmids containing the catabolic dehydroquinase gene from Neurospora crassa.
N. K. Alton (1978)
10.3389/fmicb.2015.00478
The Min system and other nucleoid-independent regulators of Z ring positioning
Veronica W Rowlett (2015)
Minicells ofBacillus subtilis
J. Reeve (1973)
10.1128/AAC.34.12.2331
Correlation of quinolone MIC and inhibition of DNA, RNA, and protein synthesis and induction of the SOS response in Escherichia coli.
L. Piddock (1990)
10.1128/JB.120.3.1274-1278.1974
Asymmetry and extent of in vivo transcripition of R-plasmid deoxyribonucleic acid in Escherichia coli.
D. Vapnek (1974)
10.1007/978-3-642-66062-7_26
The Relation of a Tetracycline-Induced R Factor Membrane Protein to Tetracycline Resistance
S. Levy (1975)
10.1007/BF00332622
Coding properties of cloned nitrogenase structural genes from Rhizobium japonicum
M. Fuhrmann (2004)
10.1128/mSphere.00428-18
Minicells as a Damage Disposal Mechanism in Escherichia coli
C. Rang (2018)
10.1016/0005-2787(76)90263-X
Characterization of a replication mutant of the bacteriocinogenic plasmid Clo DF13.
E. Veltkamp (1976)
10.1007/978-3-642-70586-1_14
Genetic analysis of Streptococcus mutans virulence.
R. Curtiss (1985)
10.1016/0005-2787(78)90246-0
Expression of bacteriophage M13 dna in vivo. I. Synthesis of phage-specific RNA and protein in minicells.
M. Smits (1978)
10.1093/PLANKT/19.2.209
Bacterivory in seawater samples estimated by a dual radioactive-labelling technique
M. Zubkov (1997)
10.1128/AAC.32.6.932
Antiseptic and antibiotic resistance plasmid in Staphylococcus aureus that possesses ability to confer chlorhexidine and acrinol resistance.
T. Yamamoto (1988)
10.14264/UQL.2015.174
Evaluation of chimeric virus-like particles, capsomeres and bacterial minicells as vaccine delivery platforms
David J. Pattinson (2014)
10.1099/00221287-111-2-387
Isolation by differential and zonal centrifugation of minicells segregated by Escherichia coli.
G. R. Barker (1979)
10.1016/0022-2836(84)90352-8
A 37 X 10(3) molecular weight plasmid-encoded protein is required for replication and copy number control in the plasmid pSC101 and its temperature-sensitive derivative pHS1.
K. Armstrong (1984)
See more
Semantic Scholar Logo Some data provided by SemanticScholar