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Expression Of The Gene Encoding Protein A In Staphylococcus Aureus And Coagulase-negative Staphylococci

M. Uhlén, B. Guss, B. Nilsson, F. Götz, M. Lindberg
Published 1984 · Biology, Medicine

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Two shuttle vectors containing the gene for protein A (spa) from Staphylococcus aureus have been constructed to study expression of the gene in various strains of S. aureus and in the coagulase-negative species Staphylococcus epidermidis, Staphylococcus capitis, and Staphylococcus xylosus. One plasmid, pSPA15, contains the complete structural gene for protein A, which binds to the cell wall in various Staphylococcus species. The other plasmid, pSPA16, codes for a truncated protein A lacking the C-terminal part called region X. The latter is exclusively extracellular in all Staphylococcus species tested, which confirms the importance of region X for cell wall binding. The expression of the plasmid-coded protein A in various strains of S. aureus is strongly correlated to the expression of the chromosomal spa gene. The coagulase-negative species expressing plasmid-encoded protein A produce 12 to 30% of the amount coded by the chromosomal spa gene in S. aureus strains Cowan I and A676.
This paper references
10.1099/00221287-33-1-121
ANALYSIS BY TRANSDUCTION OF MUTATIONS AFFECTING PENICILLINASE FORMATION IN STAPHYLOCOCCUS AUREUS.
R. Novick (1963)
“Protein A” from S. Aureus I. Pseudo-Immune Reaction with Human γ-Globulin
A. Forsgren (1966)
10.1159/000229699
A method of trace iodination of proteins for immunologic studies.
P. McConahey (1966)
Protein A" from S. aureus
A. Forsgren (1966)
A method of trace
P J Mcconahey (1966)
A method of trace J. BACT'ERIOL. EXPRESSION OF spa IN STAPHYLOCOCCI AND E. COLI 719 iodination of proteins for immunologic studies
P. J. McConahey (1966)
Protein A" from S
Sjoquist. (1966)
10.1016/0022-2836(69)90288-5
A complementation analysis of the restriction and modification of DNA in Escherichia coli.
H. Boyer (1969)
A complementation
H. W. Boyer (1969)
10.1038/227680A0
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
U. K. Laemmli (1970)
Phylogenetic insight into evolution of mammalian Fc fragment of gamma G globulin using staphylococcal protein A.
G. Kronvall (1970)
Quantitation of staphylococcal protein A: Determination of equilibrium constant and number of protein A residues on bacteria.
G. Kronvall (1970)
10.1128/jb.107.1.245-250.1971
Protein A Mutants of Staphylococcus aureus
A. Forsgren (1971)
10.1111/J.1432-1033.1972.TB02024.X
Some Physicochemical Properties of Protein A from Staphylococcus aureus
I. Björk (1972)
10.1111/J.1432-1033.1972.TB02086.X
Localization of protein A in the bacteria.
J. Sjöquist (1972)
10.1111/J.1699-0463.1972.TB00197.X
Correlation between the occurrence of protein A and some other properties in Staphylococcus aureus.
I. Lind (1972)
10.1128/jb.115.1.139-145.1973
Plasmid-Specific Transformation in Staphylococcus aureus
M. Lindberg (1973)
10.1111/J.1699-0463.1973.TB02199.X
Sensitized sheep red cells as a reactant for Staphylococcus aureus protein A. Methodology and epidemiology with special reference to weakly reacting methicillin-resistant strains.
S. Winblad (1973)
10.1128/jb.120.2.679-686.1974
Transduction and Plasmid Deoxyribonucleic Acid Analysis in a Multiply Antibiotic-Resistant Strain of Staphylococcus epidermidis
L. Rosendorf (1974)
10.1099/00207713-25-1-62
Isolation and Characterization of Staphylococci from Human Skin II. Descriptions of Four New Species: Staphylococcus warneri, Staphylococcus capitis, Staphylococcus hominis, and Staphylococcus simulans1
W. Kloos (1975)
10.1128/jb.124.2.597-601.1975
Recombinant plasmid obtained from two different, compatible staphylococcal plasmids
S. Iordanescu (1975)
10.1099/00207713-25-1-50
Isolation and Characterization of Staphylococci from Human Skin I. Amended Descriptions of Staphylococcus epidermidis and Staphylococcus saprophyticus and Descriptions of Three New Species: Staphylococcus cohnii, Staphylococcus haemolyticus, and Staphylococcus xylosus
K. Schleifer (1975)
10.1128/jb.123.3.905-915.1975
Transformation reveals a chromosomal locus of the gene(s) for methicillin resistance in Staphylococcus aureus
J. Sjöström (1975)
Isolation and characterization of staphylococci from human skin
K. H. Schleifer (1975)
10.1111/J.1432-1033.1976.TB10788.X
Formation of extracellular protein A by Staphylococcus aureus.
J. Movitz (1976)
10.1111/J.1432-1033.1977.TB11760.X
Structural studies on the four repetitive Fc-binding regions in protein A from Staphylococcus aureus.
J. Sjödahl (1977)
10.1111/J.1432-1033.1977.TB11431.X
Extracellular protein A from a methicillin-resistant strain of Staphylococcus aureus.
R. Lindmark (1977)
10.1111/J.1432-1033.1977.TB11324.X
Repetitive Sequences in Protein A from Staphylococcus aureus
J. Sjödahl (1977)
10.1073/PNAS.75.3.1433
DNA cloning in Bacillus subtilis.
S. Ehrlich (1978)
10.1016/0022-1759(78)90259-4
Use of staphylococcal protein A as an immunological reagent.
J. Goding (1978)
10.1128/jb.133.2.614-620.1978
Effects of streptomycin and novobiocin on Staphylococcus aureus gene expression
K. Nordström (1978)
Sjoquist . 1966 . " Protein A " from S . aureus . 1 . Pseudoimmune reaction with human - y - globulin
J. W. Goding (1978)
Effects of streptomycin
K. Nordstrom (1978)
10.1128/jcm.10.5.752-753.1979
Occurrence of protein A in Staphylococcus aureus and closely related Staphylococcus species
R. Lachica (1979)
10.1016/0076-6879(79)68023-0
Transformation and preservation of competent bacterial cells by freezing.
D. Morrison (1979)
10.1016/0147-619X(79)90052-0
Transformation of Staphylococcus aureus by heterologous plasmids.
J. Sjöström (1979)
10.1093/NAR/7.6.1513
A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
H. Birnboim (1979)
10.1111/j.1348-0421.1979.tb00441.x
Physico‐ and Immunochemical Properties of Staphylococcal Protein A Extracellularly Produced by a Set of Mutants from Staphylococcus aureus Cowan I
J. Movitz (1979)
10.1111/J.1574-6941.1980.TB01626.X
MUTANTS OF STAPHYLOCOCCUS AUREUS AFFECTED IN THE REGULATION OF EXOPROTEIN SYNTHESIS
A. Björklind (1980)
10.1016/0378-1119(80)90025-6
DNA cloning in Bacillus subtilis. III. Efficiency of random-segment cloning and insertional inactivation vectors.
B. Michel (1980)
10.1128/jb.145.1.74-81.1981
Plasmid transfer and genetic recombination by protoplast fusion in staphylococci
F. Götz (1981)
10.1016/S0174-3031(81)80103-5
Protein A-Aktivität von Staphylococcus hyicus im Vergleich zu Protein A von Staphylococcus aureus
H. Müller (1981)
10.1093/NAR/9.16.4087
pUR222, a vector for cloning and rapid chemical sequencing of DNA.
U. Rüther (1981)
Genetic studies in Staphylcocccus aureus using protoplasts: cell fusion and transformation, p. 535-541. in J. Jeljaszewicz (ed.), Staphylococci and staphylococcal infections
M. Lindberg (1981)
Genetic studies in Staphylcocccus aureus using protoplasts: cell fusion
M. Lindberg (1981)
Genetic studies in Staphylcocccus aureus using protoplasts: cell fusion and transformation
M Lindberg (1981)
Protein A of Staphylococcus aureus and related immunoglobulin receptors produced by streptococci and pneumonococci.
J. J. Langone (1982)
10.1016/S0065-2776(08)60722-1
Protein A of Staphylococcus aureus and related immunoglobulin receptors produced by streptococci and pneumonococci.
Langone Jj (1982)
10.1111/J.1699-0463.1982.TB00105.X
Properties of the serum factor involved in the precipitation reaction with Staphylococcus capitis.
A. Osland (1982)
Molecular cloning. Cold Spring Harbor Laboratory
T Maniatis (1982)
Molecular cloning. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y
T. Maniatis (1982)
10.1016/0378-1119(83)90025-2
Gene fusion vectors based on the gene for staphylococcal protein A.
M. Uhlén (1983)
10.1073/PNAS.80.3.697
Gene for staphylococcal protein A.
S. Löfdahl (1983)
Cloning and expression of the Staphylococcus aureus protein A gene in Escherichia coli.
C. J. Duggleby (1983)
10.1093/NAR/11.10.3065
Cloning and expression of the staphyiococcus aureus protein A gene in Escheirchia Coli
C. J. Duggleby (1983)
10.1016/s0021-9258(17)43463-6
Complete sequence of the staphylococcal gene encoding protein A. A gene evolved through multiple duplications.
M. Uhlén (1984)
10.1111/J.1432-1033.1984.TB07931.X
Region X, the cell-wall-attachment part of staphylococcal protein A.
B. Guss (1984)
Genetic studies in Staphylcocccus aureus using protoplasts : cell fusion and transformation , p . 535 - 541
J. Jeljaszewicz



This paper is referenced by
10.1128/AEM.01435-20
Protein A-Mediated Binding of Staphylococcus spp. to Antibodies in Flow Cytometric Assays and Reduction of This Binding by Using Fc Receptor Blocking Reagent
U. Cronin (2020)
10.1128/MICROBIOLSPEC.GPP3-0012-2018
Surface Proteins on Gram-Positive Bacteria.
V. Fischetti (2019)
NoteInhibition of the Bacterial Surface Sortase by Medicinal Plants ProteinAnchoring TranspeptidaseJSsu
Soo-Whan Kim (2018)
10.1101/245522
Septal Secretion of Protein A in Staphylococcus aureus Requires SecA and Lipoteichoic Acid Synthesis
Wenqi Yu (2018)
10.1093/chromsci/bmy070
Covalent Immobilization of Protein A on Chitosan and Aldehyde Double-Branched Chitosan as Biocompatible Carriers for Immunoglobulin G (Igg) Purification.
Simin Khodaei (2018)
Characterization of Protein A and Sortase A in Staphylococcus pseudintermedius : Potential Targets for Novel Therapeutic Approaches
M. Balachandran (2017)
10.1371/journal.pone.0148383
Intramammary Immunization of Pregnant Mice with Staphylococcal Protein A Reduces the Post-Challenge Mammary Gland Bacterial Load but Not Pathology
J. Gogoi-Tiwari (2016)
10.25419/RCSI.10808855.V1
Trespassing the defensive lines cardiovascular infection
C. McDonnell (2016)
10.1128/mBio.00538-16
Staphylococcus aureus Protein A Mediates Interspecies Interactions at the Cell Surface of Pseudomonas aeruginosa
Catherine R. Armbruster (2016)
Development of methods for the detection Staphylococcal Protein A in ICU patient urine
C. Varley (2016)
10.1007/s13205-016-0397-7
Protein fusion tags for efficient expression and purification of recombinant proteins in the periplasmic space of E. coli
A. Malik (2016)
10.1007/978-1-4939-1755-6_14
Tandem affinity purification to identify cytosolic and nuclear gβγ-interacting proteins.
Rhiannon Campden (2015)
10.1021/bc400440u
Site-specific photoconjugation of antibodies using chemically synthesized IgG-binding domains.
Anna Perols (2014)
Identification and characterisation of cell division proteins in Staphylococcus aureus
Azhar F Kabli (2013)
Population structure, host cell interactions and pathogenesis of Staphylococcus aureus strains isolated at Tygerberg hospital, South Africa
W. Oosthuysen (2013)
Detection, Identification and Susceptibility Testing of Bacteria by Flow
Lutz Thomas Zabel (2013)
10.4172/2155-9597.S5-005
Detection, Identification and Susceptibility Testing of Bacteria by Flow Cytometry
S. Nuding (2013)
Structural and functional studies of a Staphylococcus aureus surface protein
Andrew S. Brentnall (2012)
Molecular mechanisms underlying staphylococcal induced osteomyelitis.
T. Claro (2012)
10.1371/journal.pone.0040586
Staphylococcus aureus Protein A Plays a Critical Role in Mediating Bone Destruction and Bone Loss in Osteomyelitis
A. Widaa (2012)
10.1371/journal.pone.0018748
Staphylococcus aureus Protein A Binds to Osteoblasts and Triggers Signals That Weaken Bone in Osteomyelitis
T. Claro (2011)
Small molecule inhibition of Staphylococcus aureus virulence
Erin K. Sully (2011)
10.1016/j.mimet.2008.10.004
The use of molecular beacons to directly measure bacterial mRNA abundances and transcript degradation.
Lisa J Kuechenmeister (2009)
Effect of eleutherine americana Merr. Bulb extracts on food poisoning staphylococcus aureus and its application in food systems
Ifesan Beatrice Olawumi Temilade (2009)
10.1128/JB.01222-08
Protein A-Mediated Multicellular Behavior in Staphylococcus aureus
N. Merino (2009)
Genital tract CD4 + T cells for vaccination and protection against Chlamydia trachomatis
Ellen Marks (2009)
10.1007/978-1-4020-8412-6_16
Immunity Against Chlamydia trachomatis
E. Marks (2008)
Novel applications of shotgun phage display
A. Rosander (2004)
10.1271/bbb.66.2751
Inhibition of the Bacterial Surface Protein Anchoring Transpeptidase Sortase by Medicinal Plants
S. Kim (2002)
10.1128/9781555817992.CH6
Cell Wall-Anchored Surface Proteins and Lipoproteins of Gram-Positive Bacteria
S. Mazmanian (2002)
10.1007/0-306-46848-4_9
Surface Protein Anchoring and Display in Staphylococci
H. Ton-That (2001)
10.1126/SCIENCE.285.5428.760
Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall.
S. Mazmanian (1999)
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