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Cadmium-resistant Mutant Of Bacillus Subtilis 168 With Reduced Cadmium Transport.

R. Laddaga, R. Bessen, Sandra Silver
Published 1985 · Biology, Medicine

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Cd2+ and Mn2+ accumulation was studied with wild-type Bacillus subtilis 168 and a Cd2+-resistant mutant. After 5 min of incubation in the presence of 0.1 microM 109Cd2+ or 54Mn2+, both strains accumulated comparable amounts of 54Mn2+, while the sensitive cells accumulated three times more 109Cd2+ than the Cd2+-resistant cells did. Both 54Mn2+ and 109Cd2+ uptake, which apparently occur by the same transport system, demonstrated cation specificity; 20 microM Mn2+ or Cd2+ (but not Zn2+) inhibited the uptake of 0.1 microM 109Cd2+ or 54Mn2+. 54Mn2+ and 109Cd2+ uptake was energy dependent and temperature sensitive, but 109Cd2+ uptake in the Cd2+-resistant strain was only partially inhibited by an uncoupler or by a decrease in temperature. 109Cd2+ uptake in the sensitive strain followed Michaelis-Menten kinetics with a Km of 1.8 microM Cd2+ and a Vmax of 1.5 mumol/min X g (dry weight); 109Cd2+ uptake in the Cd2+-resistant strain was not saturable. The apparent Km value for the saturable component of 109Cd2+ uptake by the Cd2+-resistant strain was very similar to that of the sensitive strain, but the Vmax was 25 times lower than the Vmax for the sensitive strain. The Km and Vmax for 54Mn2+ uptake by both strains were very similar. Cd2+ inhibition of 54Mn2+ uptake had an apparent Ki of 3.4 and 21.5 microM Cd2+ for the sensitive and Cd2+-resistant strains, respectively. Mn2+ had an apparent Ki of 1.2 microM Mn2+ for inhibition of 109Cd2+ uptake by the sensitive strain, but the Cd2+-resistant strain had no defined Ki value for inhibition of Cd2+ uptake by Mn2+.



This paper is referenced by
10.1139/M86-085
Cadmium transport, resistance, and toxicity in bacteria, algae, and fungi.
J. Trevors (1986)
10.1128/AEM.65.11.4741-4745.1999
Characterization of Cadmium Uptake in Lactobacillus plantarum and Isolation of Cadmium and Manganese Uptake Mutants
Z. Hao (1999)
Zinc, cadmium and lead resistance mechanisms in bacteria and their contribution to biosensing
A. Hynninen (2010)
10.1021/BI047318E
High-resolution crystal structure of manganese peroxidase: substrate and inhibitor complexes.
M. Sundaramoorthy (2005)
10.1099/00221287-131-10-2539
Cadmium Resistance in Pseudomonas putida: Growth and Uptake of Cadmium
D. P. Higham (1985)
10.1007/BF01062217
Plasmid mediated metal and antibiotic resistance in marinePseudomonas
D. Rani (2005)
10.1128/AEM.65.11.4746-4752.1999
Cloning, Expression, and Characterization of Cadmium and Manganese Uptake Genes from Lactobacillus plantarum
Z. Hao (1999)
10.1016/0147-619X(92)90006-V
Plasmid-mediated resistance to tellurite: expressed and cryptic.
E. Walter (1992)
10.1139/W03-053
Mechanisms of cadmium resistance in anaerobic bacterial enrichments degrading pentachlorophenol.
S. R. Kamashwaran (2003)
10.1080/09593338709384526
Adaptation to cadmium in a sensitive marine pseudomonad
G. Flatau (1987)
10.1016/S0065-2911(08)60158-7
Metal-microbe interactions: contemporary approaches.
T. J. Beveridge (1997)
10.1046/J.1432-1327.2000.01173.X
Effects of cadmium on manganese peroxidase competitive inhibition of MnII oxidation and thermal stabilization of the enzyme.
H. Youngs (2000)
10.1620/TJEM.196.43
Cellular cadmium uptake mediated by the transport system for manganese.
S. Himeno (2002)
10.1271/bbb.63.1463
Toxicity of Cadmium Particle Dust in Bacterial Cells.
N. Yoshida (1999)
Investigating the Effects of Cadmium on Rumen Microbial Fermentation and Nutrient Digestibility Using Gas Production
Fazel Almasi (2013)
10.1016/0734-9750(87)90006-1
Metal resistance and accumulation in bacteria.
B. H. Belliveau (1987)
10.1016/0923-2508(96)81389-1
Identification of a high-molecular-weight cadmium-binding protein in copper-resistant Bacillus acidocaldarius cells.
C. Capasso (1996)
10.1128/JB.181.6.1939-1943.1999
SodA and manganese are essential for resistance to oxidative stress in growing and sporulating cells of Bacillus subtilis.
T. Inaoka (1999)
10.1016/B978-0-12-596935-2.50007-4
Bacterial Magnesium, Manganese, and Zinc Transport
S. Silver (1987)
10.1016/0147-619X(92)90003-S
Resistance to cadmium, cobalt, zinc, and nickel in microbes.
D. Nies (1992)
10.1016/0098-8472(94)90043-4
Transport and toxicity of cadmium: its regulation in the cyanobacterium Synechocystis aquatilis
B. Pawlik (1994)
10.1007/BF01568397
Genetic mapping of cadmium resistance mutations inBacillus subtilis
Dr. Daniel R. Zeigler (2005)
10.1007/s002840010307
Reduced Uptake as a Mechanism of Zinc Tolerance in Oscillatoria anguistissima
P. Ahuja (2001)
10.1128/AEM.64.11.4610-4613.1998
Metal Toxicity Reduction in Naphthalene Biodegradation by Use of Metal-Chelating Adsorbents
P. Malakul (1998)
10.1007/s002530051457
Microbial heavy-metal resistance
D. Nies (1999)
10.1111/J.1574-6968.1986.TB01673.X
Cadmium-induced loss of surface polyphosphate in Acinetobacter lwoffi
N. Suresh (1986)
10.1016/j.biortech.2009.04.037
Adsorption kinetics of Pb and Cd by two plant growth promoting rhizobacteria.
S. Wu (2009)
10.1201/9781420032048.SEC3
Microbial Genomics as an Integrated Tool for Developing Biosensors for Toxic Trace Elements in the Environment
R. Chakraborty (2005)
The impact of heavy metals on the aerobic biodegradation of 1,2-dichloroethane in soil.
Adhika. Balgobind (2009)
10.1007/978-94-011-2274-0_8
Molecular biology and biotechnology of microbial interactions with organic and inorganic heavy metal compounds.
G. M. Gadd (1992)
10.1074/JBC.271.42.26057
Manganese Transport in the Cyanobacterium Synechocystis sp. PCC 6803*
V. Bartsevich (1996)
10.1139/B91-168
A cadmium-tolerant strain of Neocosmospora vasinfecta shows reduced cadmium influx
K. Budd (1991)
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