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Helicobacter Pylori PorCDAB And OorDABC Genes Encode Distinct Pyruvate:flavodoxin And 2-oxoglutarate:acceptor Oxidoreductases Which Mediate Electron Transport To NADP.

N. Hughes, C. Clayton, P. Chalk, D. J. Kelly
Published 1998 · Medicine, Biology

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Helicobacter pylori, a major cause of human gastric disease, is a microaerophilic bacterium that contains neither pyruvate nor 2-oxoglutarate dehydrogenase activity. Previous studies (N. J. Hughes, P. A. Chalk, C. L. Clayton, and D. J. Kelly, J. Bacteriol. 177:3953-3959, 1995) have indicated that the major routes for the generation of acetyl coenzyme A (acetyl-CoA) and succinyl-CoA are via pyruvate:flavodoxin oxidoreductase (POR) and 2-oxoglutarate:acceptor oxidoreductase (OOR), respectively. The purified POR is a heterotetrameric protein, with subunits of 48 (PorA), 36 (PorB), 24 (PorC), and 14 (PorD) kDa. In this study OOR has been purified, and it is similarly composed of polypeptides of 43 (OorA), 33 (OorB), 24 (OorC), and 10 (OorD) kDa. Both POR and OOR are oxygen labile and are likely to be major contributors to the microaerophilic phenotype of H. pylori. Unlike POR, OOR was unable to use a previously identified flavodoxin (FldA) as an electron acceptor. Although the purified enzymes were unable to reduce NAD(P), electrons from both pyruvate and 2-oxoglutarate could reduce NADP in cell extracts, consistent with a role for these oxidoreductases in the provision of NADPH as a respiratory electron donor. The H. pylori por, oor, and fldA genes were cloned and sequenced. The deduced por gene products showed significant sequence similarity to archaeal four-subunit 2-oxoacid:acceptor oxidoreductases. However, the amino acid sequences of OorA and -B were more closely related to that of the two-subunit POR of the aerobic halophile Halobacterium halobium. Both porD and oorD encode integral ferredoxin-like subunits. POR and OOR are probably essential enzymes in H. pylori, as insertion inactivation of porB and oorA appeared to be lethal.
This paper references
10.1016/0167-4838(93)90190-3
Purification and characterization of pyruvate ferredoxin oxidoreductase from the hyperthermophilic archaeon Pyrococcus furiosus.
J. Blamey (1993)
10.1016/0014-5793(89)81064-6
A common structural motif in thiamin pyrophosphate‐binding enzymes
C. Hawkins (1989)
10.1136/bmj.302.6788.1302
Association between infection with Helicobacter pylori and risk of gastric cancer: evidence from a prospective investigation.
D. Forman (1991)
10.1111/J.1574-6968.1995.TB07553.X
Kinetics of substrate oxidation by whole cells and cell membranes of Helicobacter pylori.
H. Chang (1995)
10.1111/J.1432-1033.1992.TB16792.X
Improved purification, crystallization and primary structure of pyruvate:ferredoxin oxidoreductase from Halobacterium halobium.
W. Plaga (1992)
10.1111/J.1574-6968.1991.TB04619.X
Evidence for a pentose phosphate pathway in Helicobacter pylori
G. Mendz (1991)
10.1128/JB.178.16.4822-4829.1996
Metabolic activities of metronidazole-sensitive and -resistant strains of Helicobacter pylori: repression of pyruvate oxidoreductase and expression of isocitrate lyase activity correlate with resistance.
P. Hoffman (1996)
10.1111/J.1432-1033.1981.TB05376.X
Purification and properties of two 2-oxoacid:ferredoxin oxidoreductases from Halobacterium halobium.
L. Kerscher (1981)
10.1021/BI00170A019
Characterization of an ancestral type of pyruvate ferredoxin oxidoreductase from the hyperthermophilic bacterium, Thermotoga maritima.
J. Blamey (1994)
10.1111/J.1574-6968.1996.TB08365.X
Hydrogen uptake hydrogenase in Helicobacter pylori.
R. Maier (1996)
10.1016/S0065-3233(08)60362-9
Oxidoreductase-type enzymes and redox proteins involved in fermentative metabolisms of hyperthermophilic Archaea.
M. Adams (1996)
10.1007/BF00262199
Pyruvate: ferredoxin oxidoreductase from the sulfate-reducing Archaeoglobus fulgidus: molecular composition, catalytic properties, and sequence alignments
J. Kunow (2004)
10.1093/NAR/16.23.11379
The nucleotide sequence of the nifJ gene of Klebsiella pneumoniae.
M. Cannon (1988)
10.1111/J.1432-1033.1982.TB06569.X
Routes of flavodoxin and ferredoxin reduction in Escherichia coli. CoA-acylating pyruvate: flavodoxin and NADPH: flavodoxin oxidoreductases participating in the activation of pyruvate formate-lyase.
H. P. Blaschkowski (1982)
10.1128/JB.178.1.248-257.1996
Molecular and phylogenetic characterization of pyruvate and 2-ketoisovalerate ferredoxin oxidoreductases from Pyrococcus furiosus and pyruvate ferredoxin oxidoreductase from Thermotoga maritima.
A. Kletzin (1996)
THE ROLE OF INFECTIOUS AGENTS IN PEPTIC ULCER DISEASE
G. Tytgat (1993)
10.1016/S1874-6047(08)60225-5
2 Flavodoxins and Electron-Transferring Flavoproteins
S. Mayhew (1975)
10.1099/13500872-140-8-2085
Metabolism of pyruvate and glucose by intact cells of Helicobacter pylori studied by 13C NMR spectroscopy.
P. Chalk (1994)
10.1128/JB.172.8.4715-4718.1990
Conservation of hydrogenase and polyferredoxin structures in the hyperthermophilic archaebacterium Methanothermus fervidus.
V. J. Steigerwald (1990)
10.1038/42323
Italian universities move to peer review for grants
A. Abbott (1997)
10.1093/INFDIS/157.3.465
Restriction endonuclease analysis of the genome of Campylobacter pylori with a rapid extraction method: evidence for considerable genomic variation.
S. I. Majewski (1988)
10.1146/ANNUREV.MI.42.100188.003441
Plasmid-mediated heavy metal resistances.
S. Silver (1988)
10.1139/M95-027
Pyruvate:ferredoxin oxidoreductase in Campylobacter species
J. Daucher (1995)
Electron transport to nitrogenase. Purification and characterization of pyruvate:flavodoxin oxidoreductase. The nifJ gene product.
V. K. Shah (1983)
Results of a Multicentre European survey in 1991 of metronidazole resistance in Helicobacter pylori. European Study Group on Antibiotic Susceptibility of Helicobacter pylori
N. Figura (1992)
10.1136/jcp.41.2.207
Prevention of nitroimidazole resistance in Campylobacter pylori by coadministration of colloidal bismuth subcitrate: clinical and in vitro studies.
C. Goodwin (1988)
10.1006/ABBI.1994.1319
The Entner-Doudoroff pathway in Helicobacter pylori.
G. Mendz (1994)
10.1038/42321
Microsoft ‘in talks’ to set up research base in Cambridge
E. Masood (1997)
10.1016/0378-1097(95)00130-W
Kinetics of substrate oxidation by whole cells and cell membranes of
Huai-Tzu Chang (1995)
Purification and characterization of urease from Helicobacter pylori.
B. Dunn (1990)
10.1128/JB.178.20.5890-5896.1996
Characterization of a fourth type of 2-keto acid-oxidizing enzyme from a hyperthermophilic archaeon: 2-ketoglutarate ferredoxin oxidoreductase from Thermococcus litoralis.
X. Mai (1996)
10.1093/JAC/31.1.9
Nitroimidazole drugs--action and resistance mechanisms. I. Mechanisms of action.
D. I. Edwards (1993)
10.1128/JB.177.14.3953-3959.1995
Identification of carboxylation enzymes and characterization of a novel four-subunit pyruvate:flavodoxin oxidoreductase from Helicobacter pylori.
N. Hughes (1995)
10.1002/j.1460-2075.1985.tb04120.x
In vivo transfer of genetic information between gram‐positive and gram‐negative bacteria.
P. Trieu-Cuot (1985)
Free radical intermediates in the reaction of pyruvate:ferredoxin oxidoreductase in Tritrichomonas foetus hydrogenosomes.
R. Docampo (1987)
10.1056/NEJM199110173251603
Helicobacter pylori infection and the risk of gastric carcinoma.
J. Parsonnet (1991)
10.1007/PL00008607
Indolepyruvate ferredoxin oxidoreductase from Pyrococcus sp. KOD1 possesses a mosaic structure showing features of various oxidoreductases
M. A. Siddiqui (1997)
10.1093/JAC/39.3.347
Oxygen scavenging, NADH oxidase and metronidazole resistance in Helicobacter pylori.
M. Smith (1997)
10.1128/JB.178.11.3365-3368.1996
Purification and characterization of 2-oxoglutarate:ferredoxin oxidoreductase from a thermophilic, obligately chemolithoautotrophic bacterium, Hydrogenobacter thermophilus TK-6.
K. S. Yoon (1996)
10.1007/BF00258146
Identification of the nifJ gene coding for pyruvate: ferredoxin oxidoreductase in dinitrogen-fixing cyanobacteria
O. Schmitz (2004)
10.1016/0378-1119(90)90463-2
Chloramphenicol resistance in Campylobacter coli: nucleotide sequence, expression, and cloning vector construction.
Y. Wang (1990)
Cloning and characterization of a 12-gene cluster from Bacillus subtilis encoding nine enzymes for de novo purine nucleotide synthesis.
D. Ebbole (1987)
10.1038/41483
The complete genome sequence of the gastric pathogen Helicobacter pylori
Jean-F. Tomb (1997)



This paper is referenced by
Procede de production fermentative de pyruvate
M. Bott (2002)
10.3390/antibiotics9050213
Antibacterial Discovery: 21st Century Challenges
P. Hoffman (2020)
10.1099/00221287-144-9-2505
Structural, functional and mutational analysis of the pfr gene encoding a ferritin from Helicobacter pylori.
S. Bereswill (1998)
10.1128/9781555815530.CH7
Campylobacter Pathogenomics: Genomes and Beyond
D. Fouts (2007)
10.1016/S0924-8579(01)00468-X
Metronidazole resistance in Helicobacter pylori.
P. Jenks (2002)
10.1128/MMBR.63.3.642-674.1999
Metabolism and Genetics of Helicobacter pylori: the Genome Era
A. Marais (1999)
10.1111/j.1523-5378.2006.00413.x
Is Helicobacter pylori a True Microaerophile?
S. Bury-Moné (2006)
Scalable bio-production of high value products in bacteria
Yukie Kawasaki (2015)
10.1038/35001088
The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences
J. Parkhill (2000)
Chapter 17 FUNCTIONAL GENOMICS AND ENZYME EVOLUTION Homologous and Analogous Enzymes Encoded in Microbial
Michael Y. Galperin (1999)
10.1016/S0167-4838(02)00280-7
Substrate recognition by 2-oxoacid:ferredoxin oxidoreductase from Sulfolobus sp. strain 7.
Eriko Fukuda (2002)
10.1128/9781555815554.CH3
Complexity and Versatility in the Physiology and Metabolism of Campylobacter jejuni
D. Kelly (2008)
Microbial nitrogen cycle interactions in laboratory-scale model systems
L. Russ (2015)
10.1586/eri.09.61
Novel Helicobacter pylori therapeutic targets: the unusual suspects
M. Duckworth (2009)
10.1128/JB.182.17.4704-4710.2000
Metabolic pathways for cytotoxic end product formation from glutamate- and aspartate-containing peptides by Porphyromonas gingivalis.
N. Takahashi (2000)
10.1128/9781555818005.CH12
The Citric Acid Cycle and Fatty Acid Biosynthesis
D. J. Kelly (2001)
10.1128/aac.45.5.1522-1527.2001
Inhibitory Activities of Lansoprazole against Respiration in Helicobacter pylori
K. Nagata (2001)
10.1016/s0005-2728(01)00230-4
Succinate:quinone oxidoreductases from ϵ-proteobacteria11Dedicated to Achim Kröger on the occasion of his 65th birthday
C. Lancaster (2002)
10.1371/journal.pbio.0040383
The Genome of Deep-Sea Vent Chemolithoautotroph Thiomicrospira crunogena XCL-2
K. Scott (2006)
10.1016/S0005-2728(01)00230-4
Succinate:quinone oxidoreductases from epsilon-proteobacteria.
C. Lancaster (2002)
Isothiocyanate on Campylobacter jejuni Insights into the Mode of Action of Benzyl
Christine Baysse (2013)
10.1128/JB.182.11.3204-3209.2000
Another unusual type of citric acid cycle enzyme in Helicobacter pylori: the malate:quinone oxidoreductase.
B. Kather (2000)
10.1016/B978-0-12-804007-2.00027-8
Proteomics Analyses Applied to the Human Foodborne Bacterial Pathogen Campylobacter spp.
O. Tresse (2017)
10.1128/JB.01647-07
The Campylobacter jejuni NADH:ubiquinone oxidoreductase (complex I) utilizes flavodoxin rather than NADH.
D. Weerakoon (2008)
Polychaete , a Hydrothermal Vent Alvinella pompejana Bacterial Community Associated with Evidence of Chemolithoautotrophy in the
Barbara J. Campbell (2003)
10.14288/1.0167574
Campylobacter jejuni metabolism in survival and host cell interactions
Mark Pryjma (2014)
10.1016/J.IJHYDENE.2014.08.015
Hydrogen evolution catalyzed by viable and non-viable cells on biocathodes
M. Yates (2014)
10.1128/JB.182.18.5082-5090.2000
Sequential inactivation of rdxA (HP0954) and frxA (HP0642) nitroreductase genes causes moderate and high-level metronidazole resistance in Helicobacter pylori.
J. Y. Jeong (2000)
10.1099/MIC.0.26247-0
The essential role of fumarate reductase in haem-dependent growth stimulation of Bacteroides fragilis.
A. Baughn (2003)
10.1128/JB.185.20.6119-6129.2003
2-Oxoglutarate:NADP(+) oxidoreductase in Azoarcus evansii: properties and function in electron transfer reactions in aromatic ring reduction.
C. Ebenau-Jehle (2003)
10.1128/AEM.69.9.5070-5078.2003
Evidence of Chemolithoautotrophy in the Bacterial Community Associated with Alvinella pompejana, a Hydrothermal Vent Polychaete
B. Campbell (2003)
Mutations in Helicobacter pylori porD and oorD genes may contribute to furazolidone resistance.
Z. Su (2006)
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