Online citations, reference lists, and bibliographies.
← Back to Search

The Alternative Sigma Factor RpoN Is Required For HrpActivity In Pseudomonas Syringae Pv. Maculicola And Acts At The Level Of HrpL Transcription

Erik L. Hendrickson, Pablo Guevera, Frederick M. Ausubel

Cite This
Download PDF
Analyze on Scholarcy
Share
ABSTRACT β-Glucuronidase (uidA) reporter gene fusions were constructed for the hrpZ, hrpL, andhrpS genes from the phytopathogen Pseudomonas syringae pv. maculicola strain ES4326. These reporters, as well as an avrRpt2-uidA fusion, were used to measure transcriptional activity in ES4326 and a ES4326 rpoNmutant. rpoN was required for the expression ofavrRpt2, hrpZ, and hrpL in vitro in minimal media and in vivo when infiltrated into Arabidopsis thaliana leaves. In contrast, the expression of hrpSwas essentially the same in wild-type and rpoN mutant strains. Constitutive expression of hrpL in anrpoN mutant restored hrpZ transcription to wild-type levels, restored the hypersensitive response when infiltrated into tobacco (Nicotiana tobacum), and partially restored the elicitation of virulence-related symptoms but not growth when infiltrated into Arabidopsis leaves. These data indicate that rpoN-mediated control of hrp gene expression acts at the level of hrpL and that in planta growth of P. syringae is not required for the elicitation of disease symptoms.