Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Composite Circular Forms Of R Factor Deoxyribonucleic Acid Molecules.

T. Nisioka, M. Mitani, R. Clowes
Published 1969 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Two R factors, one (R15) conferring resistance to streptomycin and sulfonamide (SM(r)SU(r)) and the other (222/R3) to streptomycin, sulfonamide, and chloramphenicol (SM(r)SU(r)CM(r)), were transferred to a Proteus mirabilis strain, and deoxyribonucleic acid (DNA) extracted from these strains was subjected to density-gradient centrifugation. R15-DNA formed a single satellite band at a density of 1.709 g cm(-3). Electron microscopy of samples from this band showed circular molecules of one type, with a contour length of 18 mum (35 x 10(6) daltons). 222/R3-DNA formed a satellite band with three peaks at densities 1.708, 1.711 and 1.717 g cm(-3). Electron micrographs revealed circular structures from each band with contour lengths, respectively, of 29 (54 x 10(6) daltons), 36 (68 x 10(6) daltons), and 6 mum (12 x 10(6) daltons). "Supertwisted" forms of several molecular species were found. It is suggested that 222/R3 DNA comprises either a single 36-mum molecule or two individual molecules, 29 and 6 mum in length, and that this may reflect the evolutionary development of R factors.
This paper references
Facteurs de transfert et résistance aux antibiotiques chez les entérobactéries.
E. Anderson (1967)
10.1128/jb.81.5.679-683.1961
Episome-mediated transfer of drug resistance in Enterobacteriaceae. II. Elimination of resistance factors with acridine dyes.
T. Watanabe (1961)
10.1128/MMBR.32.1.55-83.1968
Phylogenetic relationships of drug-resistance factors and other transmissible bacterial plasmids.
E. Meynell (1968)
10.1016/S0022-2836(66)80149-3
Molecular nature of the drug-resistance factors of the Enterobacteriaceae.
R. Rownd (1966)
10.1073/PNAS.58.4.1461
Chromosome transfer between Escherichia coli HFR strains and Proteus mirabilis.
P. Gemski (1967)
Zygose létale dans des croisements entre souches colicinogènes et non colicinogènes d'Escherichia coli.
L. Alfoldi (1957)
10.1016/S0022-2836(67)80024-X
Electron microscopy of size and shape of viral DNA in solutions of different ionic strengths.
D. Lang (1967)
10.1073/PNAS.58.4.1731
Circular DNA forms of a bacterial sex factor.
F. T. Hickson (1967)
Complementary structure of interacting molecules at the ends of lambda DNA molecules
F. T. Hickson (1965)
In Macromolecular metabolism, The New York Heart
J. Vinograd (1967)
10.1128/JB.81.5.669-678.1961
Episome-mediated transfer of drug resistance in Enterobacteriaceae. I. Transfer of resistance factors by conjugation.
T. Watanabe (1961)
10.1073/PNAS.46.1.57
THE EFFECT OF ACRIDINE DYES ON MATING TYPE FACTORS IN ESCHERICHIA COLI.
Y. Hirota (1960)
10.1099/00221287-36-3-365
TRANSFER OF THE COLICIN I FACTOR IN ESCHERICHIA COLI K12 AND ITS INTERACTION WITH THE F FERTILITY FACTOR.
M. Monk (1964)
The nature of intergeneric epi - somal infection
N. Datta (1961)
Sex-Specific Bacteriophages of Escherichia coli K12.
R. Dettori (1961)
10.1128/jb.88.3.716-726.1964
EPISOME-MEDIATED TRANSFER OF DRUG RESISTANCE IN ENTEROBACTERIACEAE. VII. TWO TYPES OF NATURALLY OCCURRING R FACTORS.
T. Watanabe (1964)
10.1016/S0022-2836(66)80097-9
The molecular nature of R-factors.
S. Falkow (1966)
La sessulita nei batteri
L. L. Cavalli (1950)



This paper is referenced by
10.1128/AAC.9.1.77
Elimination of Resistance Determinants from R-Factor R1 by Intercalative Compounds
F. E. Hahn (1976)
10.1016/S0022-2836(76)80124-6
Involvement of multiple translocating DNA segments and recombinational hotspots in the structural evolution of bacterial plasmids.
D. Kopecko (1976)
Isolation andCharacterization oftheFertility Factor PofVibrio cholerae
A. Datta (1973)
10.1073/PNAS.67.2.510
Non-chromosomal antibiotic resistance in bacteria. 3. Isolation of the discrete transfer unit of the R-factor R1.
S. Cohen (1970)
10.1007/BF00267320
Propeties of plasmids produced by recombination between R factors of groups J and FII
R. Hedges (2004)
10.1007/978-1-4615-8966-2_5
Physical-Chemical Methods for the Detection of the Effect of Mutagens on DNA*
B. Strauss (1971)
10.1007/BF00269586
The isolation of IS1 and IS2 DNA
Friedrich Georg Dr Schmidt (2004)
10.1007/BF00431594
Involvement of IS1 in the dissociation of the r-determinant and RTF components of the plasmid R100.1
M. Chandler (2004)
Molecular NatureofR-Factor Deoxyribonucleic AcidIsolated fromSalmonella typhimurium Minicells
A. Perry (1973)
10.1007/BF00446911
Expression and stability of Escherichia coli F-prime factors in Proteus mirabilis
E. Morgan (2004)
10.1111/j.1749-6632.1971.tb30652.x
THE ORIGIN OF R FACTORS *
T. Watanabe (1971)
10.1038/2241273A0
Multiple Molecular Species of Circular R-factor DNA isolated from Escherichia coll
S. Cohen (1969)
10.1021/BI00724A005
Strand and site specificity of the relaxation event for the relaxation complex of the antibiotic resistance plasmid R6K.
Y. M. Kupersztoch-Portnoy (1974)
10.1126/SCIENCE.176.4036.758
Transmissible multiple drug resistance in Enterobacteriaceae.
J. Davies (1972)
10.1038/227606A0
Segregation of Transferable R Factors into Escherichia coli Minicells
S. Levy (1970)
10.1007/978-3-7091-8405-9
Drug Receptor Interactions in Antimicrobial Chemotherapy
J. Drews (1975)
10.1007/978-3-642-81064-0_21
Identification and Characterization of a New Resistance Transfer Factor Isolated from Escherichia Coli
F. Schöffl (1977)
10.1007/978-3-642-66800-5_5
Gene cloning with small plasmids.
J. Collins (1977)
10.1128/JB.153.2.921-929.1983
Analysis of tetracycline resistance encoded by transposon Tn10: deletion mapping of tetracycline-sensitive point mutations and identification of two structural genes.
D. Coleman (1983)
10.1016/0022-2836(70)90092-6
Non-chromosomal antibiotic resistance in bacteria. II. Molecular nature of R-factors isolated from Proteus mirabilis and Escherichia coli.
S. Cohen (1970)
10.1146/ANNUREV.GE.11.120177.000535
DNA rearrangements in procaryotes.
P. Starlinger (1977)
10.1128/MMBR.33.2.210-263.1969
Extrachromosomal inheritance in bacteria.
R. Novick (1969)
10.1111/j.1749-6632.1971.tb30665.x
ELIMINATION OF BACTERIAL EPISOMES BY DNA‐COMPLEXING COMPOUNDS
F. E. Hahn (1971)
10.25772/GEGD-0834
THE BIOLOGICAL AND STRUCTURAL CHARACTERIZATION OF TRANSFERABLE BACTEROIDES R PLASMIDS
R. Welch (1979)
Physical Properties andMechanism ofTransfer of R Factors inEscherichia coli
D. Vapnek (1971)
10.1016/S0079-6603(08)60104-X
Resistance factors and their ecological importance to bacteria and to man.
M. Richmond (1973)
10.1007/BF00332820
A bacteriocinogenic factor of Enterobacter cloacae
G. A. Tieze (2004)
10.1111/j.1749-6632.1971.tb30656.x
THE MOLECULAR NATURE AND REPLICATION OF DRUG RESISTANCE FACTORS OF THE ENTEROBACTERIACEAE *
R. Rownd (1971)
Dissociation andInteraction ofindividual Components ofaDegradative Plasmid Aggregate inPseudomonas
A. Chakrabarty (1974)
10.1002/JOBM.19740140710
Prinzipien und Möglichkeiten der Genamplifikation bei Bakterien
D. Noack (1974)
Molecular and Genetic Properties of Plasmid R 485 Conferring Resistance to Sulphonamides
J.. ()
10.1007/978-3-7091-8405-9_7
Molecular Aspects of the Biosynthesis of R Factor DNA
R. Clowes (1975)
See more
Semantic Scholar Logo Some data provided by SemanticScholar