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Rapid Group-, Serotype-, And Vaccine Strain-Specific Identification Of Poliovirus Isolates By Real-Time Reverse Transcription-PCR Using Degenerate Primers And Probes Containing Deoxyinosine Residues

David R. Kilpatrick, Chen-Fu Yang, Karen Ching, Annelet Vincent, Jane Iber, Ray Campagnoli, Mark Mandelbaum, Lina De, Su-Ju Yang, Allan Nix, Olen M. Kew

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ABSTRACT We have adapted our previously described poliovirus diagnostic reverse transcription-PCR (RT-PCR) assays to a real-time RT-PCR (rRT-PCR) format. Our highly specific assays and rRT-PCR reagents are designed for use in the WHO Global Polio Laboratory Network for rapid and large-scale identification of poliovirus field isolates.