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Mitochondrial DNA Structure And Expression In Specialized Subtypes Of Mammalian Striated Muscle.

B. Annex, R. Williams
Published 1990 · Chemistry, Biology, Medicine

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Mitochondrial DNA (mt DNA) in cells of vertebrate organisms can assume an unusual triplex DNA structure known as the displacement loop (D loop). This triplex DNA structure forms when a partially replicated heavy strand of mtDNA (7S mtDNA) remains annealed to the light strand, displacing the native heavy strand in this region. The D-loop region contains the promoters for both heavy- and light-strand transcription as well as the origin of heavy-strand replication. However, the distribution of triplex and duplex forms of mtDNA in relation to respiratory activity of mammalian tissues has not been systematically characterized, and the functional significance of the D-loop structure is unknown. In comparisons of specialized muscle subtypes within the same species and of the same muscle subtype in different species, the relative proportion of D-loop versus duplex forms of mtDNA in striated muscle tissues of several mammalian species demonstrated marked variation, ranging from 1% in glycolytic fast skeletal fibers of the rabbit to 65% in the mouse heart. There was a consistent and direct correlation between the ratio of triplex to duplex forms of mtDNA and the capacity of these tissues for oxidative metabolism. The proportion of D-loop forms likewise correlated directly with mtDNA copy number, mtRNA abundance, and the specific activity of the mtDNA (gamma) polymerase. The D-loop form of mtDNA does not appear to be transcribed at greater efficiency than the duplex form, since the ratio of mtDNA copy number to mtRNA was unrelated to the proportion of triplex mtDNA genomes. However, tissues with a preponderance of D-loop forms tended to express greater levels of cytochrome b mRNA relative to mitochondrial rRNA transcripts, suggesting that the triplex structure may be associated with variations in partial versus full-length transcription of the heavy strand.
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This paper is referenced by
10.1093/gerona/glq109
Molecular adaptations to aerobic exercise training in skeletal muscle of older women.
A. Konopka (2010)
Inter-tissue differences in mitochondrial enzyme activity, RNA and DNA in rainbow trout (Oncorhynchus mykiss)
Leary (1998)
10.1007/978-1-4615-4843-0_28
Age-Linked Changes in the Genotype and Phenotype of Mitochondria
M. Gadaleta (1999)
10.1101/030205
Age-related and heteroplasmy-related variation in human mtDNA copy number
Manja Wachsmuth (2016)
10.1074/JBC.273.6.3447
Differential Expression of Mitochondrial DNA Replication Factors in Mammalian Tissues*
R. Schultz (1998)
10.1016/S0300-9084(00)88881-1
Aging and mitochondria.
M. Gadaleta (1998)
10.1152/JAPPL.2001.90.3.1137
Invited Review: contractile activity-induced mitochondrial biogenesis in skeletal muscle.
D. Hood (2001)
10.3109/13693786.2011.572930
RNA interference of WdFKS1 mRNA expression causes slowed growth, incomplete septation and loss of cell wall integrity in yeast cells of the polymorphic, pathogenic fungus Wangiella (Exophiala) dermatitidis.
Pengfei Guo (2011)
10.1002/CPHY.CP120125
Regulation of Gene Expression in Skeletal Muscle by Contractile Activity
R. S. Williams (2011)
mitochondrial DNA D-loop region . termination-associated sequence in the Protein binding to a single
C. S. Madsen (1993)
10.1111/apha.13004
The guardian of the genome p53 regulates exercise‐induced mitochondrial plasticity beyond organelle biogenesis
W. Smiles (2018)
10.1093/nar/gkp614
The accessory subunit of mitochondrial DNA polymerase γ determines the DNA content of mitochondrial nucleoids in human cultured cells
M. Di Re (2009)
Structure and biophysical studies of mitochondrial Transcription Factor A in complex with DNA
Anna Cuppari (2016)
10.1007/BF00315776
Selective DNA amplification regulates transcript levels in plant mitochondria
Robin C. Muise (2004)
10.1006/BBRC.1997.8052
Multiple protein-binding sites in the TAS-region of human and rat mitochondrial DNA.
M. Roberti (1998)
10.1093/NAR/30.9.2004
Release of replication termination controls mitochondrial DNA copy number after depletion with 2',3'-dideoxycytidine.
T. Brown (2002)
10.1073/pnas.1600537113
Pathological ribonuclease H1 causes R-loop depletion and aberrant DNA segregation in mitochondria
Gokhan Akman (2016)
10.1007/978-88-470-0376-7_7
Mitochondria: The Dark Side
D. Edgar (2007)
10.1016/0169-5347(94)90176-7
Thermal habit, metabolic rate and the evolution of mitochondrial DNA.
D. M. Rand (1994)
AGEING – THE ROLE OF MITOCHONDRIA IN DETERMINATION OF CAENORHABDITIS ELEGANS LIFE
Ivana Bratić Hench (2011)
10.1016/0005-2728(93)90170-K
Regulation of the expression of mitochondrial proteins: relationship between mtDNA copy number and cytochrome-c oxidase activity in human cells and tissues.
C. Van den Bogert (1993)
10.1016/j.jtbi.2012.08.044
Overlapping genes coded in the 3'-to-5'-direction in mitochondrial genes and 3'-to-5' polymerization of non-complementary RNA by an 'invertase'.
H. Seligmann (2012)
REVIEW REGULATION OF MUSCLE MITOCHONDRIAL DESIGN
C. Moyes (1998)
10.1006/BBRC.2000.3501
Identification of a novel human mitochondrial D-loop RNA species which exhibits upregulated expression following cellular immortalization.
E. Duncan (2000)
10.1017/S0016672305007718
A large-scale screening of the normalized mammalian mitochondrial gene expression profiles.
S. Anisimov (2005)
10.1371/journal.pgen.1005939
Age-Related and Heteroplasmy-Related Variation in Human mtDNA Copy Number
Manja Wachsmuth (2016)
10.1007/0-306-46869-7_56
Detection of Mitochondrial Regulatory Region RNA in Cultured Cells and Differentiated Tissue Cells: The Implications for Cellular Growth Control
N. Nakamichi (2002)
10.1111/j.1749-6632.1992.tb27453.x
Age‐Dependent Structural Variations in Rat Brain Mitochondrial DNA a
V. Petruzzella (1992)
10.1093/nar/gkz277
The mitochondrial R-loop
I. Holt (2019)
Hood mitochondrial biogenesis in skeletal muscle
David A. Hood (2001)
10.1074/jbc.275.10.7430
A Conserved Mechanism for Controlling the Translation of β-F1-ATPase mRNA between the Fetal Liver and Cancer Cells*
M. L. de Heredia (2000)
10.3390/cells8070686
Mitochondrial Homeostasis and Cellular Senescence
P. Vasileiou (2019)
See more
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