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Novel Mode Of Microbial Energy Metabolism: Organic Carbon Oxidation Coupled To Dissimilatory Reduction Of Iron Or Manganese.
D. Lovley, E. J. Phillips
Published 1988 · Chemistry, Medicine
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A dissimilatory Fe(III)- and Mn(IV)-reducing microorganism was isolated from freshwater sediments of the Potomac River, Maryland. The isolate, designated GS-15, grew in defined anaerobic medium with acetate as the sole electron donor and Fe(III), Mn(IV), or nitrate as the sole electron acceptor. GS-15 oxidized acetate to carbon dioxide with the concomitant reduction of amorphic Fe(III) oxide to magnetite (Fe(3)O(4)). When Fe(III) citrate replaced amorphic Fe(III) oxide as the electron acceptor, GS-15 grew faster and reduced all of the added Fe(III) to Fe(II). GS-15 reduced a natural amorphic Fe(III) oxide but did not significantly reduce highly crystalline Fe(III) forms. Fe(III) was reduced optimally at pH 6.7 to 7 and at 30 to 35 degrees C. Ethanol, butyrate, and propionate could also serve as electron donors for Fe(III) reduction. A variety of other organic compounds and hydrogen could not. MnO(2) was completely reduced to Mn(II), which precipitated as rhodochrosite (MnCO(3)). Nitrate was reduced to ammonia. Oxygen could not serve as an electron acceptor, and it inhibited growth with the other electron acceptors. This is the first demonstration that microorganisms can completely oxidize organic compounds with Fe(III) or Mn(IV) as the sole electron acceptor and that oxidation of organic matter coupled to dissimilatory Fe(III) or Mn(IV) reduction can yield energy for microbial growth. GS-15 provides a model for how enzymatically catalyzed reactions can be quantitatively significant mechanisms for the reduction of iron and manganese in anaerobic environments.
This paper references
Bacteriology of manganese nodules. IV. Induction of an MnO2-reductase system in a marine bacillus.
R. Trimble (1970)
A New Geochemical Classification of Sedimentary Environments
R. Berner (1981)
A serum bottle modification of the Hungate technique for cultivating obligate anaerobes.
T. Miller (1974)
Reduction of ferric iron by heterotrophic bacteria in lake sediments
J. Jones (1984)
[Anaerobic reduction of ferric iron by hydrogen bacteria].
V. V. Balashova (1979)
Bacteriology of manganese nodules: III. Reduction of MnO(2) by two strains of nodule bacteria.
R. Trimble (1968)
Activities of aerobic and anaerobic bacteria in lake sediments and their effect on the water column , p . 107 - 145
J. G. Jones (1982)
Diagenesis of Fe and S in Amazon inner shelf muds: apparent dominance of Fe reduction and implications for the genesis of ironstones
R. Aller (1986)
Characterization of iron-reducing Alteromonas putrefaciens strains from oil field fluids
K. Semple (1987)
Bioconversion of organic carbon to CH4 and CO2
M. Wolin (1987)
Bacterial Reduction of Ferric Iron in a Stratified Eutrophic Lake
J. Jones (1983)
Manganese reduction by a marine Bacillus species.
J. D. de Vrind (1986)
Determination of ferric and ferrous iron in oxalate extracts of sediment
F. N. Ponnamperuma (1987)
Competitive mechanisms for inhibition of sulfate reduction and methane production in the zone of ferric iron reduction in sediments.
D. Lovley (1987)
Inhibitor studies of dissimilative Fe(III) reduction by Pseudomonas sp. strain 200 ("Pseudomonas ferrireductans")
R. Arnold (1986)
Phosphorus release from lake sediments as affected by chironomids
G. W. Gallepp (1978)
Reduction of iron and synthesis of protoheme by Spirillum itersonii and other organisms.
H. Dailey (1977)
Fructose degradation by Desulfovibrio sp . in pure culture and in cocul - ture with Methanospirillum hungatii
H. A. Dailey (1986)
Capacity for denitrification and reduction of nitrate to ammonia in a coastal marine sediment.
J. Sørensen (1978)
Anaerobes pumping iron
R. Frankel (1987)
Interactions between methanogenic and sulfate-reducing bacteria in sediments
D. M. Ward (1985)
Rates of Biogeochemical Processes in Anoxic Sediments
William S. Reeburgh (1983)
Trace element sorption by sediments and soil - sites and processes , p . 425 - 553
W. Chappel (1977)
Utilization of amino acids as energy substrates by two marine Desulfovibrio strains
A. Stams (1985)
The dissimila - tory sulfate - reducing bacteria , p . 926 - 940
M. P. Starr (1981)
The Chemistry of Submerged Soils
F. N. Ponnamperuma (1972)
Microbial manganese reduction by enrichment cultures from coastal marine sediments.
D. Burdige (1985)
Methanogens: reevaluation of a unique biological group.
W. Balch (1979)
The Roll-Tube Method for Cultivation of Strict Anaerobes
R. Hungate (1969)
Electron transport components of the MnO2 reductase system and the location of the terminal reductase in a marine Bacillus.
W. Ghiorse (1976)
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Bacterial cycling of minerals that affect plant growth in waterlogged soils: a review
H. J. Laanbroek (1990)
Microbial manganese reduction mediated by bacterial strains isolated from aquifer sediments
J. DiRuggiero (2006)
A review of apatites, iron and manganese minerals and their roles as indicators of biological activity in black shales
H. Skinner (1993)
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R. Musgrave (1995)
The potential for metal release by reductive dissolution of weathered mine tailings
I. Ribeta (1995)
Fe(III) and S0 reduction by Pelobacter carbinolicus.
D. Lovley (1995)
Desulfuromonas palmitatis sp. nov., a marine dissimilatory Fe(III) reducer that can oxidize long-chain fatty acids
J. Coates (1995)
Vanadium(V) Reduction by Shewanella oneidensis MR-1 Requires Menaquinone and Cytochromes from the Cytoplasmic and Outer Membranes
J. Myers (2004)
Dynamics of landfill gas migration in unconsolidated sands
G. M. Williams (1999)
Subsurface Chemistry of Nitroaromatic Compounds
R. Schwarzenbach (2000)
THE APPLICATION OF HRTEM TECHNIQUES AND NANOSIMS TO CHEMICALLY AND ISOTOPICALLY CHARACTERIZE GEOBACTER SULFURREDUCENS SURFACES
M. Fayek (2005)
Surface chemistry and acid-base activity of Shewanella putrefaciens: Cell wall charging and metal binding to bacterial cell walls
J. Claessens (2006)
Reactive iron(III) in sediments: Chemical versus microbial extractions
C. Hyacinthe (2006)
Unique Microbial Community in Drilling Fluids from Chinese Continental Scientific Drilling
G. Zhang (2006)
Kinetic modeling of microbially-driven redox chemistry of subsurface environments : coupling transport, microbial metabolism and geochemistry
K. Hunter (1998)
The influence of iron reduction on the reductive biotransformation of pentachloronitrobenzene
D. O. Taş (2007)
Sorptive interaction between goethite and strongly reducing organic substances from anaerobic decomposition of green manures
Qingman Li (2008)
Effects of ferric iron reduction and regeneration on nitrous oxide and methane emissions in a rice soil.
Bin Huang (2009)
Extracellular Electron Shuttle Mediated Biodegradation of Hexahydro-1,3,5-trinitro-1,3,5-triazine
M. J. Kwon (2009)
Analysis of chitin particle size on maximum power generation, power longevity, and Coulombic efficiency in solid–substrate microbial fuel cells
F. Rezaei (2009)
Power Generation and Electrochemical Analysis of Biocathode Microbial Fuel Cell Using Graphite Fibre Brush as Cathode Material
Shijie You (2009)
Simultaneous removal of sulfide and organics with vanadium(V) reduction in microbial fuel cells
B. Zhang (2009)
Influence of the Iron-Reducing Bacteria on the Release of Heavy Metals in Anaerobic River Sediment
C. Gounou (2010)
Microbial fuel cell cathodes with poly(dimethylsiloxane) diffusion layers constructed around stainless steel mesh current collectors.
F. Zhang (2010)
Metal reduction at cold temperatures by Shewanella isolates from various marine environments
R. D. Stapleton (2005)
Anaerobic degradation of tetrachlorobisphenol-A in river sediment
S. Y. Yuan (2011)
Exploring Bacterial Nanowires: From Properties to Functions and Implications
Kar Man Leung (2011)
HYPORHEIC ZONE PROCESS CONTROLS ON DISSOLVED ORGANIC CARBON QUALITY
P. Gabrielsen (2012)
Shewanella oneidensis MR-1 chemotaxis proteins and electron-transport chain components essential for congregation near insoluble electron acceptors.
H. W. Harris (2012)
Biomobilization of heavy metals from the sediments affect the bacterial population of Al-Ghadir River (Lebanon)
Amale Mcheik (2013)
Fervidicola ferrireducens gen. nov., sp. nov., a thermophilic anaerobic bacterium from geothermal waters of the Great Artesian Basin, Australia.
C. D. Ogg (2009)
Concentration responses of toxicity sensor with Shewanella oneidensis MR-1 growing in bioelectrochemical systems.
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