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Temperature Sensitivity Of The Penicillin-induced Autolysis Mechanism In Nongrowing Cultures Of Escherichia Coli

W Kusser, E E Ishiguro

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The effect of incubation temperature on the ampicillin-induced autolysis of nongrowing Escherichia coli was determined. The autolysis mechanisms in amino acid-deprived relA mutant cells treated with chloramphenicol were temperature sensitive. This temperature-sensitive autolysis was demonstrated in three independent ways: turbidimetric determinations, viable cell counts, and solubilization of radiolabeled peptidoglycan.