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A Rapid And Simple Method For Extracting Human Immunodeficiency Virus Type 1 RNA From Plasma: Enhanced Sensitivity

J Mulder, R Resnick, B Saget, S Scheibel, S Herman, H Payne, R Harrigan, S Kwok

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We present here a rapid and simple technique for processing human immunodeficiency virus (HIV)-infected plasma by high-speed centrifugation. HIV type 1 virions are pelleted from up to 1 ml of plasma, gently lysed with a nonionic detergent, and directly amplified. The procedure has few manipulations and requires approximately 1.5 h for the processing of 24 samples. Viral recovery ranges from 80 to 90%, with an analytical sensitivity approaching 20 copies/ml.