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Estimation Of Methods Of Protein Delivery Into Mammalian Cells — A Comparative Study By Electroporation And Bioporter Assay

R. Todorova
Published 2009 · Biology, Medicine

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To test the efficiency of some alternative methods for protein delivery into live cells were used rEGFP (recombinant Enhanced Green Fluorescent Protein) and HeLa cells as model system. The compared methods, electroporation (up to 10%) and Bioporter (up to 20%), deliver proteins and peptides to the cells with efficiency, enough for functional studies of mammalian cells. Thus, the delivery by either of both methods, followed by fluorescence activated cell sorting (FACS) of the cell population, can give a sufficient number of uniformly loaded cells for different studies including transcription assays and gene therapy.
This paper references
10.1016/S0168-3659(02)00417-0
Gene packaging with lipids, peptides and viruses inhibits transfection by electroporation in vitro.
Arlena L Coulberson (2003)
Promoters containing ATF-binding sites are de-regulated in cells that express the EWS/ATF1 oncogene.
A. D. Brown (1995)
Lipid-mediated protein delivery of suicide nucleoside kinases.
X. Zheng (2003)
10.1002/(SICI)1097-0320(19960701)24:3<226::AID-CYTO5>3.0.CO;2-F
Delivery of macromolecules into adherent cells via electroporation for use in fluorescence spectroscopic imaging and metabolic studies.
G. Bright (1996)
Phospholipase C-gamma 1 associates with viral and cellular src kinases.
O. Nakanishi (1993)
10.1016/0022-1759(94)90400-6
Efficient transfer of antibodies into mammalian cells by electroporation.
J. Lukas (1994)
10.1016/S0022-1759(02)00554-9
A method for functional evaluation of caspase activation pathways in intact lymphoid cells using electroporation-mediated protein delivery and flow cytometric analysis.
E. Eksioglu-Demiralp (2003)
Regular ArticleIn Vitro Tyrosine Phosphorylation of PLC-γ1 and PLC-γ2 by SRC-Family Protein Tyrosine Kinases
Fang Liao (1993)
10.1089/10849780152752092
Augmentation of antiproliferative and antitumor effect on human cancer cells in combined use of electroporation with a plant toxin, saporin.
H. Mashiba (2001)
10.1016/j.bbrc.2008.02.089
Neuroprotective function of human neuroglobin is correlated with its guanine nucleotide dissociation inhibitor activity.
S. Watanabe (2008)
10.1007/BF02921607
applications of electroporation of adherent cellsIn Situ, on a partly conductive slide
L. Raptis (1995)
10.1074/JBC.270.26.15734
Electroporation of pp60c−src Antibodies Inhibits the Angiotensin II Activation of Phospholipase C-γ1 in Rat Aortic Smooth Muscle Cells (*)
M. Marrero (1995)
10.1016/J.ADDR.2004.10.005
Transmembrane delivery of protein and peptide drugs by TAT-mediated transduction in the treatment of cancer.
J. Wadia (2005)
10.1016/0076-6879(83)01033-2
[30] Microinjection of tissue culture cells
M. Graessmann (1983)
10.1016/0092-8674(82)90087-3
Introduction of macromolecules into cultured mammalian cells by osmotic lysis of pinocytic vesicles
C. Okada (1982)
Electroporation of peptides into adherent cells in situ.
L. Raptis (1995)
10.1007/0-306-46803-4_8
Transdermal peptide delivery using electroporation.
R. Potts (1997)



This paper is referenced by
10.1117/1.JBO.19.1.015003
Evaluation of parameters influencing the molecular delivery by biodegradable microsphere-mediated perforation using femtosecond laser
Tatsuki Mitsuhashi (2014)
10.3390/ijms20174254
Delivery of Mixed-Lineage Kinase Domain-Like Protein by Vapor Nanobubble Photoporation Induces Necroptotic-Like Cell Death in Tumor Cells
L. Van Hoecke (2019)
10.1016/j.jbiosc.2013.03.006
Intracellular protein cyclization catalyzed by exogenously transduced Streptococcus pyogenes sortase A.
J. Zhang (2013)
Development and Characterization of Lifeact - a versatile marker for the visualization of F-actin
Julia Riedl (2011)
10.1007/s40259-020-00419-w
Applying Antibodies Inside Cells: Principles and Recent Advances in Neurobiology, Virology and Oncology
Congcong Zhang (2020)
10.1016/j.ccr.2010.09.007
A genetic defect in exportin-5 traps precursor microRNAs in the nucleus of cancer cells.
S. Melo (2010)
10.3109/10717544.2011.600783
Comparative analysis of the methods of drug and protein delivery for the treatment of cancer, genetic diseases and diagnostics
R. Todorova (2011)
10.4161/mabs.29268
Delivery of antibodies to the cytosol
Andrea L. J. Marschall (2014)
10.1117/12.2077604
Plasmonic cell manipulation for biomedical and screening applications
D. Heinemann (2015)
10.7275/pmkg-tj22
Polymeric Peptide Mimics for Protein Delivery
Coralie M. Backlund (2018)
10.1088/0957-4484/25/24/245101
Delivery of proteins to mammalian cells via gold nanoparticle mediated laser transfection.
D. Heinemann (2014)
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