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Comparison Of Impregnated Bone Morphogenetic Protein-2 Release Kinetics From Biopolymer Scaffolds

A. V. Vasilyev, T. Bukharova, V. S. Kuznetsova, Y. D. Zagoskin, S. A. Minaeva, T. Grigoriev, E. N. Antonov, E. O. Osidak, E. V. Galitsyna, I. I. Babichenko, S. Domogatsky, V. Popov, S. N. Chvalun, D. Gol’dshtein, A. Kulakov
Published 2019 · Materials Science

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The purpose of this study was to evaluate the release kinetics of impregnated recombinant human bone morphogenetic protein-2 (rhBMP-2) from different engineered scaffolds. Poly(lactide-co-glycolide) (PLG) matrices prepared by supercritical fluid technologies (SCFT) showed the highest biocompatibility and long-term release of rhBMP-2. There was an even release of rhBMP-2 from them for 11 days. The subsequent use of laser sintering allowed delaying the peak of the protein release for a period of 13 to 15 days. The average loss of rhBMP-2 using SCFT did not exceed 20%. The maximum release of rhBMP-2 from a collagen-fibronectin hydrogel was in the period from 4 to 6 days. But 47 ± 12% rhBMP-2 loss was shown. Highly porous polylactide-based scaffolds obtained by freeze-drying were inferior to other scaffolds in their ability to release rhBMP-2 for a prolonged period. The hydrogel and chitosan-based granules showed high cytotoxicity and a short period of protein release.
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