Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Colonic Epithelium Is Diffusely Abnormal In Ulcerative Colitis And Colorectal Cancer.

P. Gibson, O. Rosella, R. Nov, G. Young
Published 1995 · Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
The hypothesis that the colonic epithelium is diffusely abnormal in ulcerative colitis was examined by comparing disease related responses in expression of markers of differentiation by colonic crypt cells to culture with and without butyrate. Cells were isolated from patients with normal colon (15), cancer (24), ulcerative colitis (19), or Crohn's disease (16). Alkaline phosphatase activities were measured in cell homogenates and the rate of glycoprotein synthesis assessed at the end of 24 hours of culture and expressed relative to the rate of protein synthesis as the G:P ratio. Alkaline phosphatase activities, but not G:P ratios, differed across the groups before and after 24 hour culture (p < 0.05), activities being lowest in the cancer group and highest in inflammatory bowel disease groups. Butyrate (1 mM) suppressed alkaline phosphatase activities in the cancer group by mean (SEM) of 17 (4) (p = 0.006) compared with no change in the other groups. Butyrate suppressed G:P ratios only in the cancer (6 (3)%, p = 0.03) and ulcerative colitis groups (5 (3)%, p = 0.04) and the changes in both were different (p < 0.05) from those in normal cells (increase of 10 (7)%). Changes in ulcerative colitis were different from those in Crohn's disease (p = 0.029). Responses were independent of the presence or absence of mucosal inflammation. These data confirm the diffuse nature of epithelial abnormalities in colorectal cancer. In ulcerative colitis, a different pattern of abnormality occurs, supporting the notion that the epithelium is also diffusely abnormal independent of mucosal inflammation.
This paper references
10.1016/0016-5085(88)90365-4
Aberrant expression of monoclonal antibody-defined colonic mucosal antigens in inflammatory bowel disease.
A. E. Haviland (1988)
10.1002/1097-0142(19880415)61:8<1563::AID-CNCR2820610813>3.0.CO;2-Y
Background mucosal changes in colorectal carcinomas.
Y. Lee (1988)
10.1152/AJPGI.1984.247.4.G419
Hepatic clearance of rat plasma intestinal alkaline phosphatase.
G. Young (1984)
10.1016/0016-5085(89)91549-7
Isolation of colonic crypts that maintain structural and metabolic viability in vitro.
P. Gibson (1989)
10.1093/JNCI/81.24.1913
Detection of the tumor-associated glycoprotein antigen (TAG-72) in premalignant lesions of the colon.
B. C. Wolf (1989)
10.1159/000171340
Pathogenic factors in inflammatory bowel disease. I. Ulcerative colitis.
P. Gibson (1992)
10.1093/JNCI/66.2.413
Colon epithelium. IV. Human colon carcinogenesis. Changes in human colon mucosa adjacent to and remote from carcinomas of the colon.
A. Shamsuddin (1981)
10.1136/gut.22.8.648
Rate and pattern of epithelial cell proliferation in ulcerative colitis.
E. Serafini (1981)
10.1016/0003-2697(76)90527-3
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
10.1007/BF02051922
The starved colon—Diminished mucosal nutrition, diminished absorption, and colitis
W. Roediger (1990)
Interleukin - 8 secretion by colonic crypt cells in vitro : modulation and disease differences
PR Gibson (1994)
10.1016/S0016-5085(70)80159-7
Cell renewal in the human rectum. In vitro autoradiographic study on active ulcerative colitis.
H. Bleiberg (1970)
10.1136/gut.34.11.1552
Ileal and colonic epithelial metabolism in quiescent ulcerative colitis: increased glutamine metabolism in distal colon but no defect in butyrate metabolism.
I. Finnie (1993)
10.1016/S0016-5085(84)80055-4
Glycoprotein composition of colonic mucosa. Specific alterations in ulcerative colitis.
D. Podolsky (1984)
Short-chain fatty acids: metabolism and clinical importance
(1991)
10.1136/jcp.43.9.744
Immunohistochemical detection of abnormal cell proliferation in colonic mucosa of subjects with polyps.
R. Wilson (1990)
Pattern of epithelial cell proliferation in colorectal mucosa of normal subjects and of patients with adenomatous polyps or cancer of the large bowel.
M. Ponz de Leon (1988)
10.1136/gut.26.10.999
Crypt cell production rate in ulcerative proctocolitis: differential increments in remission and relapse.
A. Allan (1985)
10.1136/gut.35.7.969
Secretion of urokinase and plasminogen activator inhibitor-1 by normal colonic epithelium in vitro.
P. Gibson (1994)
10.1016/0016-5085(87)90021-7
Abnormal pattern of cell proliferation in the entire colonic mucosa of patients with colon adenoma or cancer.
O. T. Terpstra (1987)
10.1136/gut.34.7.926
Glycosylation and sulphation of colonic mucus glycoproteins in patients with ulcerative colitis and in healthy subjects.
H. Morita (1993)
Contrasting effects of butyrate on proliferation and differentiation of normal and neoplastic cells
GP Young
10.1016/S0016-5085(88)80015-5
Immunohistologic study of ulcerative colitis with monoclonal antibodies against tumor-associated and/or differentiation antigens.
H. Cooper (1988)
10.1136/gut.35.6.791
Serum free medium increases expression of markers of differentiation in human colonic crypt cells.
P. Gibson (1994)
Tissue carcinoembryonic antigen, dysplasia, and disease duration in colonic inflammatory bowel disease.
A. Greenstein (1982)
10.1016/0016-5085(88)90493-3
Emergence of antigenic glycoprotein structures in ulcerative colitis detected through monoclonal antibodies.
D. Podolsky (1988)
10.1111/j.1440-1746.1992.tb00956.x
Contrasting effects of butyrate on the expression of phenotypic markers of differentiation in neoplastic and non‐neoplastic colonic epithelial cells in vitro
P. Gibson (1992)
10.1016/S0016-5085(73)80160-X
Epithelial cell renewal in cultured rectal biopsies in ulcerative colitis.
G. Eastwood (1973)
10.1016/0016-5085(91)90211-3
Colonic glycoproteins in monozygotic twins with inflammatory bowel disease.
C. Tysk (1991)
10.1016/0092-8674(93)90462-Y
Suppression of apoptosis allows differentiation and development of a multipotent hemopoietic cell line in the absence of added growth factors
L. Fairbairn (1993)
10.1083/JCB.119.3.493
Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation
Y. Gavrieli (1992)
10.1111/1523-1747.EP12478519
Programmed cell death in terminally differentiating keratinocytes: role of endogenous endonuclease.
C. McCall (1991)
10.1016/0016-5085(92)91164-Y
Neoplasia and hyperplasia of large bowel: focal lesions in an abnormal epithelium.
P. Gibson (1992)
Ultrastructural abnormalities in endoscopically and histologically normal and involved colon in ulcerative colitis.
G. Delpré (1989)
10.1016/0016-5085(88)90494-5
Alterations in mucosal content of colonic glycoconjugates in inflammatory bowel disease defined by monoclonal antibodies.
D. Podolsky (1988)
10.1016/0092-8674(90)90186-I
A genetic model for colorectal tumorigenesis
E. Fearon (1990)
10.1016/0016-5085(94)90166-X
Butyrate is a potent inhibitor of urokinase secretion by normal colonic epithelium in vitro.
P. Gibson (1994)
10.1002/1097-0142(197502)35:2<413::AID-CNCR2820350217>3.0.CO;2-B
Proliferative patterns in colonic mucosa in familial polyposis
E. Deschner (1975)
10.1016/S0889-8588(18)30565-3
Biology of large bowel cancer.
J. Willson (1989)



This paper is referenced by
10.1080/003655202753387392
Phenol Toxicity and Conjugation in Human Colonic Epithelial Cells
G. Pedersen (2002)
10.1007/978-1-4939-0897-4_4
Intestinal barrier function and the brain-gut axis.
C. Alonso (2014)
10.1016/S1590-8658(00)80361-8
Pathogenesis of colorectal cancer.
M. Ponz de Leon (2000)
HAVcR-1 and the prevention of metastatic disease in human prostate cancer
Emily Telford (2019)
10.1002/ibd.3780020412
Protective Role of the Epithelium of the Small Intestine and Colon
P. Gibson (1996)
10.3389/fimmu.2013.00280
Central Role of the Gut Epithelial Barrier in the Pathogenesis of Chronic Intestinal Inflammation: Lessons Learned from Animal Models and Human Genetics
L. Pastorelli (2013)
10.1136/gut.2004.058453
Reinforcing the mucus: a new therapeutic approach for ulcerative colitis?
P. Gibson (2005)
10.1080/01652176.1998.9694970
Role of short-chain fatty acids in the hind gut.
W. von Engelhardt (1998)
10.1093/CARCIN/20.4.539
Colonic epithelial cell activation and the paradoxical effects of butyrate.
P. Gibson (1999)
10.1016/b978-0-7020-2723-9.50043-9
[Colorectal cancer in ulcerative colitis].
Kim Bøgelund Laugesen (2014)
10.1097/00054725-199606000-00006
Abnormalities of the urokinase system in colonic crypt cells from patients with ulcerative colitis.
P. Gibson (1996)
10.1023/A:1018819600645
Carbonic Anhydrase I Reduction in Colonic Mucosa of Patients with Active Ulcerative Colitis
Rosa Fonti Giovanni (2004)
10.1136/gutjnl-2015-310255
BVES regulates c-Myc stability via PP2A and suppresses colitis-induced tumourigenesis
Bobak Parang (2016)
10.1007/s00441-014-1994-6
Defects in the adherens junction complex (E-cadherin/ β-catenin) in inflammatory bowel disease
S. Mehta (2014)
10.1016/S0950-3528(97)90051-8
2 Ulcerative colitis: an epithelial disease?
P. Gibson (1997)
10.1152/AJPGI.00398.2003
Paradoxical decrease of mitochondrial DNA deletions in epithelial cells of active ulcerative colitis patients.
K. Fukushima (2004)
10.1007/978-1-4939-0897-4
Microbial Endocrinology: The Microbiota-Gut-Brain Axis in Health and Disease
M. Lyte (2014)
10.1677/JOE.0.1790405
Interleukin-1beta (IL-1beta) and IL-6 modulate insulin-like growth factor-binding protein (IGFBP) secretion in colon cancer epithelial (Caco-2) cells.
M. Street (2003)
10.5056/jnm14084
Role of Corticotropin-releasing Factor in Gastrointestinal Permeability
B. K. Rodiño-Janeiro (2015)
10.1111/j.1749-6632.2009.04045.x
The Role of JAM‐A in Inflammatory Bowel Disease: Unrevealing the Ties That Bind
S. Vetrano (2009)
ULTRASTRUCTURAL ASPECTS OF THE COLONIC EPITHELIUM IN ULCERATIVE COLITIS
FR Ovidiu (2008)
Development, validation and implementation of an in vitro model for the study of metabolic and immune function in normal and inflamed human colonic epithelium.
G. Pedersen (2015)
10.1111/j.1572-0241.1999.00869.x
It's Not Time to Put Away the Biopsy Forceps
J. Murray (1999)
10.1056/NEJMra1102942
Ulcerative colitis.
S. Danese (2011)
Reassessing this persistent theory in light of advances in molecular microbial detection and genetic pathogenesis of disease
R. Sartor (2005)
10.1016/j.phrs.2012.04.006
Exogenous alkaline phosphatase treatment complements endogenous enzyme protection in colonic inflammation and reduces bacterial translocation in rats.
P. Martínez-Moya (2012)
10.1016/B978-0-12-415847-4.00081-1
Chapter 81 – Ulcerative Colitis
I. Fuss (2015)
10.1515/hsz-2012-0204
Effects of cathepsin K deficiency on intercellular junction proteins, luminal mucus layers, and extracellular matrix constituents in the mouse colon
Maria Arampatzidou (2012)
10.1136/gut.2004.055889
Does Mycobacterium avium subspecies paratuberculosis cause Crohn’s disease?
R. Sartor (2005)
10.1002/ibd.3780020206
Abnormalities of the Urokinase System in Colonic Crypt Cells from Patients with Ulcerative Colitis
P. Gibson (1996)
10.1042/CS0940671
Effect of topical butyrate on rectal epithelial kinetics and mucosal enzyme activities.
P. Gibson (1998)
Epidemiolocal and Anatomo-Clinical Aspects of Ulcerative Colitis in a Referral Center in Cameroon and Review of Literature
G. E. Orock (2016)
See more
Semantic Scholar Logo Some data provided by SemanticScholar