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Iron Acquisition By Oral Hemolytic Spirochetes: Isolation Of A Hemin-binding Protein And Identification Of Iron Reductase Activity.
D. Scott, E. Chan, R. Siboo
Published 1996 · Biology, Medicine
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Oral anaerobic spirochetes (OAS) have been implicated in the etiology of periodontal disease. To adapt to the environment of the subgingiva, OAS must be able to acquire iron from limited sources. OAS have previously been shown not to produce siderophores but are beta-hemolytic and can bind hemin via a proteinaceous 47-kDa outer membrane sheath (OMS) receptor. Present studies show that [3H]hemin is not transported into the cytoplasm, that hemin and ferric ammonium citrate, as the sole iron sources, can support the growth of OAS and that protoporphyrin IX and Congo red are inhibitory, thereby implying an important in vivo role for hemin as an iron source. Treponema denticola ATCC 35405 produces an iron reductase. The iron reductase can reduce the central ferric iron moiety of hemin. The 47-kDa OMS hemin-binding protein has been purified to apparent homogeneity by methanol-chloroform extraction of cellular lipoproteins and the use of a hemin-agarose bead affinity column. A model of iron acquisition by OAS is presented.
This paper references
An endo-acting proline-specific oligopeptidase from Treponema denticola ATCC 35405: evidence of hydrolysis of human bioactive peptides.
P. Mäkinen (1994)
Purification and characterization of a 45 kDa hemolysin from Treponema denticola ATCC 35404.
L. Chu (1994)
Quantitative relationship of Treponema denticola to severity of periodontal disease.
L. Simonson (1988)
Siderophore production by Pseudomonas pseudomallei.
H. Yang (1991)
Bacterial penetration in the apical pocket wall of advanced human periodontitis.
R. Frank (1980)
Inhibition of superoxide production in human polymorphonuclear leukocytes by oral treponemal factors.
M. Sela (1988)
Bacteria as Risk Markers for Periodontitis.
L. Wolff (1994)
Purification and characterization of an enzyme produced by Treponema denticola capable of hydrolyzing synthetic trypsin substrates.
K. Ohta (1986)
Hemagglutination activity of Treponema denticola grown in serum-free medium in continuous culture.
F. Mikx (1992)
Reduction of exogenous ferric iron by a surface-associated ferric reductase of Listeria spp.
H. Deneer (1995)
Binding of hemin and congo red by oral hemolytic spirochetes.
D. Scott (1993)
Iron uptake from lactoferrin and transferrin by Neisseria gonorrhoeae.
W. Mckenna (1988)
Enzyme activities from eight small-sized oral spirochetes.
N. Fiehn (1986)
Reduction of iron and synthesis of protoheme by Spirillum itersonii and other organisms.
H. Dailey (1977)
Dark-field microscopic monitoring of subgingival bacteria during periodontal therapy.
M. M. Singletary (1982)
Characteristics of hemolytic and hemagglutinating activities of Treponema denticola.
D. Grenier (1991)
Suppression of human lymphocyte responses by oral spirochetes: a monocyte-dependent phenomenon.
B. Shenker (1984)
Hemin-binding property of Porphyromonas gingivalis outer membranes.
D. Grenier (1991)
Reduction of ferric iron by Listeria monocytogenes and other species of Listeria.
H. Deneer (1993)
Growth of Porphyromonas gingivalis, Treponema denticola, T. pectinovorum, T. socranskii, and T. vincentii in a chemically defined medium.
C. Wyss (1992)
The routine isolation, growth, and maintenance of the intermediate-size anaerobic oral spirochetes from periodontal pockets.
S. Cheng (1983)
Acquisition of iron by Legionella pneumophila: role of iron reductase.
W. Johnson (1991)
The Role of Spirochetes in Periodontal Disease
W. Loesche (1988)
Role of iron in bacterial infection.
J. Bullen (1978)
Enumeration of viable oral spirochetes from periodontal pockets.
Y. S. Qiu (1994)
Effect of iron regulation on expression and hemin-binding function of outer-sheath proteins from Treponema denticola.
L. Chu (1994)
Synthesis and secretion of phospholipase C by oral spirochetes.
R. Siboo (1989)
Haemagglutinating and haemolytic activity of the extracellular vesicles of Bacteroides gingivalis W50.
H. M. Kay (1990)
Characterization of hemolysis and hemoxidation activities by Treponema denticola.
L. Chu (1994)
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Taxonomy and virulence of oral spirochetes.
E. Chan (2000)
Hemin-Binding Surface Protein fromBartonella quintana
J. A. Carroll (2000)
Cloning and Expression of Two Novel Hemin Binding Protein Genes from Treponema denticola
X. Xu (2001)
Virulence factors of Treponema denticola.
K. Ishihara (2010)
Oxygen metabolism by Treponema denticola.
C. Caldwell (1999)
The periodontal war: microbes and immunity
J. Ebersole (2017)
Microbial ferric iron reductases.
I. Schröder (2003)
Construction and analysis of hemin binding protein mutants in the oral pathogen Treponema denticola.
Xiaoping Xu (2002)
Identification of a Treponema denticola OppA Homologue That Binds Host Proteins Present in the Subgingival Environment
J. Fenno (2000)
Genotypic and Phenotypic Characterization of Treponema phagedenis from Bovine Digital Dermatitis
Hector M Espiritu (2020)
Bacterial Adhesion to Host Tissues: Bacterial adhesins and adhesive structures
Michael Wilson (2002)
Potential Role for Extracellular Glutathione-Dependent Ferric Reductase in Utilization of Environmental and Host Ferric Compounds by Histoplasma capsulatum
M. M. Timmerman (2001)
New insights into the emerging role of oral spirochaetes in periodontal disease.
M.B. Visser (2011)
Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia: the "red complex", a prototype polybacterial pathogenic consortium in periodontitis.
S. Holt (2005)
Virulence factors of oral treponemes.
J. Fenno (1998)
Comparative analysis of the oral microbiota between iron-deficiency anaemia (IDA) patients and healthy individuals by high-throughput sequencing
Ranhui Xi (2019)