Online citations, reference lists, and bibliographies.
← Back to Search

Vacuolar Import Of Proteins And Organelles From The Cytoplasm.

D. Klionsky, Y. Ohsumi
Published 1999 · Medicine, Biology

Cite This
Download PDF
Analyze on Scholarcy
Share
Many cellular processes require a balance between protein synthesis and protein degradation. The vacuole/lysosome is the main site of protein and organellar turnover within the cell due to its ability to sequester numerous hydrolases within a membrane-enclosed compartment. Several mechanisms are used to deliver substrates, as well as resident hydrolases, to this organelle. The delivery processes involve dynamic rearrangements of membrane. In addition, continual adjustments are made to respond to changes in environmental conditions. In this review, we focus on recent progress made in analyzing these delivery processes at a molecular level. The identification of protein components involved in the recognition, sequestration, and transport events has begun to provide information about this important area of eukaryotic cell physiology.
This paper references
10.1016/S0966-842X(97)01156-6
Yeast peroxisomes: function and biogenesis of a versatile cell organelle.
I. J. van der Klei (1997)
10.1083/JCB.141.3.625
Peroxisome Degradation by Microautophagy in Pichia pastoris: Identification of Specific Steps and Morphological Intermediates
Y. Sakai (1998)
10.1091/MBC.10.5.1353
Glucose-induced autophagy of peroxisomes in Pichia pastoris requires a unique E1-like protein.
W. Yuan (1999)
10.1038/26335
Protein breakdown: Ubiquitous déjà vu
S. Jentsch (1998)
10.1083/JCB.136.4.803
Identification of Novel Vesicles in the Cytosol to Vacuole Protein Degradation Pathway
P. Huang (1997)
10.1016/0006-291X(89)92308-5
Nucleotide sequence ofAMS1, the structure gene of vacuolarα-mannosidase of Saccharomyces cerevisiae
T. Yoshihisa (1989)
10.1016/S0014-5793(97)00679-0
Brefeldin A interferes with peroxisomal protein sorting in the yeast Hansenula polymorpha
F. Salomons (1997)
10.1083/JCB.131.6.1727
In vitro reconstitution of cytoplasm to vacuole protein targeting in yeast
S. Scott (1995)
10.1126/SCIENCE.8385367
Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting.
P. Schu (1993)
10.1083/JCB.124.6.903
Ultrastructural analysis of the autophagic process in yeast: detection of autophagosomes and their characterization
M. Baba (1994)
10.1128/MMBR.62.1.230-247.1998
Vacuole Biogenesis in Saccharomyces cerevisiae: Protein Transport Pathways to the Yeast Vacuole
N. J. Bryant (1998)
10.1016/0014-5793(93)80398-E
Isolation and characterization of autophagy‐defective mutants of Saccharomyces cerevisiae
M. Tsukada (1993)
10.1093/emboj/17.13.3597
Aut2p and Aut7p, two novel microtubule‐associated proteins are essential for delivery of autophagic vesicles to the vacuole
T. Lang (1998)
Vam3p, a new member of syntaxin related protein, is required for vacuolar assembly in the yeast Saccharomyces cerevisiae.
Y. Wada (1997)
10.1128/JB.179.12.3875-3883.1997
AUT3, a serine/threonine kinase gene, is essential for autophagocytosis in Saccharomyces cerevisiae.
M. Straub (1997)
10.1083/JCB.137.3.609
Transport of a Large Oligomeric Protein by the Cytoplasm to Vacuole Protein Targeting Pathway
J. Kim (1997)
10.1016/0968-0004(90)90019-8
Peptide sequences that target cytosolic proteins for lysosomal proteolysis
Dice Jf (1990)
10.1016/0065-2571(86)90018-X
The lysosomal pathway of intracellular proteolysis in liver: regulation by amino acids.
G. Mortimore (1986)
10.1074/jbc.273.18.10807
Nonclassical Protein Sorting to the Yeast Vacuole*
D. Klionsky (1998)
10.1006/JMBI.1997.0925
Folding of the presequence of yeast pAPI into an amphipathic helix determines transport of the protein from the cytosol to the vacuole.
E. Martínez (1997)
10.1016/0962-8924(94)90069-8
Autophagy and related mechanisms of lysosome-mediated protein degradation.
W. Dunn (1994)
10.1247/CSF.22.501
Mutational analysis of Csc1/Vps4p: involvement of endosome in regulation of autophagy in yeast.
K. Shirahama (1997)
10.1083/JCB.128.5.779
A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast
T. Vida (1995)
10.1074/JBC.273.7.3963
Tor, a Phosphatidylinositol Kinase Homologue, Controls Autophagy in Yeast*
Takeshi Noda (1998)
Glucose-induced microautophagy in Pichia pastoris requires the alpha-subunit of phosphofructokinase.
W. Yuan (1997)
10.1083/JCB.131.3.591
Isolation and characterization of yeast mutants in the cytoplasm to vacuole protein targeting pathway
T. M. Harding (1995)
10.1111/J.1574-6968.1991.TB05128.X
Physiological studies on the utilization of oleic acid by Saccharomyces cerevisiae in relation to microbody development.
M. Evers (1991)
10.1002/YEA.320070806
Selective inactivation of alcohol oxidase in two peroxisome‐deficient mutants of the yeast Hansenula polymorpha
I. J. van der Klei (1991)
10.1016/0014-5793(94)00672-5
Isolation of autophagocytosis mutants of Saccharomyces cerevisiae
M. Thumm (1994)
Selective autophagy of peroxisomes in methylotrophic yeasts.
D. Tuttle (1993)
10.1093/emboj/16.10.2769
Novel Golgi to vacuole delivery pathway in yeast: identification of a sorting determinant and required transport component
C. R. Cowles (1997)
10.1083/JCB.119.2.301
Autophagy in yeast demonstrated with proteinase-deficient mutants and conditions for its induction
K. Takeshige (1992)
Divergent modes of autophagy in the methylotrophic yeast Pichia pastoris.
D. Tuttle (1995)
10.1074/jbc.271.30.17621
Genetic and Phenotypic Overlap between Autophagy and the Cytoplasm to Vacuole Protein Targeting Pathway*
T. M. Harding (1996)
10.1016/S0898-6568(96)00130-1
Signal transduction pathways in macroautophagy.
P. Codogno (1997)
10.1128/JB.179.4.1068-1076.1997
AUT1, a gene essential for autophagocytosis in the yeast Saccharomyces cerevisiae.
M. Schlumpberger (1997)
10.1083/JCB.119.2.287
Aminopeptidase I of Saccharomyces cerevisiae is localized to the vacuole independent of the secretory pathway
D. Klionsky (1992)
10.1074/jbc.273.39.25000
Proteins of Newly Isolated Mutants and the Amino-terminal Proline Are Essential for Ubiquitin-Proteasome-catalyzed Catabolite Degradation of Fructose-1,6-bisphosphatase of Saccharomyces cerevisiae *
M. Hämmerle (1998)
10.1083/JCB.139.7.1687
Two Distinct Pathways for Targeting Proteins from the Cytoplasm to the Vacuole/Lysosome
M. Baba (1997)
Protein and peptide binding and stimulation of in vitro lysosomal proteolysis by the 73-kDa heat shock cognate protein.
S. R. Terlecky (1992)
Regulation of microautophagy and basal protein turnover in rat liver. Effects of short-term starvation.
G. Mortimore (1988)
10.1091/MBC.10.5.1337
Apg7p/Cvt2p is required for the cytoplasm-to-vacuole targeting, macroautophagy, and peroxisome degradation pathways.
J. Kim (1999)
10.1016/S0378-1119(97)00031-0
Analyses of APG13 gene involved in autophagy in yeast, Saccharomyces cerevisiae.
T. Funakoshi (1997)
10.1042/BJ2320743
Degradative inactivation of the peroxisomal enzyme, alcohol oxidase, during adaptation of methanol-grown Candida boidinii to ethanol.
D. J. Hill (1985)
10.1093/emboj/16.8.1820
Endosomal transport function in yeast requires a novel AAA‐type ATPase, Vps4p
M. Babst (1997)
10.1016/S0955-0674(98)80068-9
Delivery of proteins and organelles to the vacuole from the cytoplasm.
S. Scott (1998)
10.1007/s002329900220
Protein Transport from the Cytoplasm into the Vacuole
D. Klionsky (1997)
10.1038/26506
A protein conjugation system essential for autophagy
N. Mizushima (1998)
Solubilization and purification of alpha-mannosidase, a marker enzyme of vacuolar membranes in Saccharomyces cerevisiae.
T. Yoshihisa (1988)
10.1016/S0014-5793(98)00266-X
Homology between a human apoptosis specific protein and the product of APG5, a gene involved in autophagy in yeast
E. M. Hammond (1998)
10.1074/jbc.270.44.26446
Catabolite Inactivation of Fructose-1,6-bisphosphatase of Saccharomyces cerevisiae
S. M. Schork (1995)
10.1002/DMR.5610050105
Mechanism and regulation of protein degradation in liver.
G. Mortimore (1989)
10.1073/PNAS.93.22.12304
Cytoplasm-to-vacuole targeting and autophagy employ the same machinery to deliver proteins to the yeast vacuole.
S. Scott (1996)
10.1091/MBC.10.5.1367
Apg7p/Cvt2p: A novel protein-activating enzyme essential for autophagy.
I. Tanida (1999)
10.1083/JCB.138.3.517
A Multispecificity Syntaxin Homologue, Vam3p, Essential for Autophagic and Biosynthetic Protein Transport to the Vacuole
T. Darsow (1997)
10.1093/OXFORDJOURNALS.JBCHEM.A021592
Acidification of vacuoles is required for autophagic degradation in the yeast, Saccharomyces cerevisiae.
N. Nakamura (1997)
10.1083/JCB.138.1.37
Aminopeptidase I Is Targeted to the Vacuole by a Nonclassical Vesicular Mechanism
S. Scott (1997)
10.1016/S0378-1119(97)00084-X
Apg1p, a novel protein kinase required for the autophagic process in Saccharomyces cerevisiae.
Akihiro Matsuura (1997)
10.1152/PHYSREV.1998.78.1.171
Components involved in peroxisome import, biogenesis, proliferation, turnover, and movement.
S. Subramani (1998)
10.1083/JCB.138.3.531
The Membrane Protein Alkaline Phosphatase Is Delivered to the Vacuole by a Route That Is Distinct from the VPS-dependent Pathway
R. Piper (1997)
10.1074/jbc.273.35.22284
Apg14p and Apg6/Vps30p Form a Protein Complex Essential for Autophagy in the Yeast, Saccharomyces cerevisiae *
S. Kametaka (1998)
10.1016/0378-1119(96)00354-X
Structural and functional analyses of APG5, a gene involved in autophagy in yeast.
S. Kametaka (1996)
10.1006/BBRC.1995.1636
Novel system for monitoring autophagy in the yeast Saccharomyces cerevisiae.
T. Noda (1995)
10.1074/JBC.273.51.33889
A New Protein Conjugation System in Human
N. Mizushima (1998)
Molecular cloning and sequencing of genomic DNA encoding aminopeptidase I from Saccharomyces cerevisiae.
Y. H. Chang (1989)
10.1016/0014-4800(85)90020-6
Uptake--microautophagy--and degradation of exogenous proteins by isolated rat liver lysosomes. Effects of pH, ATP, and inhibitors of proteolysis.
J. Ahlberg (1985)
10.1091/MBC.8.11.2307
A novel RING finger protein complex essential for a late step in protein transport to the yeast vacuole.
S. Rieder (1997)
10.1128/JB.177.2.357-363.1995
Isolation and characterization of mutants impaired in the selective degradation of peroxisomes in the yeast Hansenula polymorpha.
V. Titorenko (1995)
10.1016/0014-5793(89)81510-8
Yeast vacuolar aminopeptidase yscI
R. Cueva (1989)
10.1126/science.273.5274.501
A Receptor for the Selective Uptake and Degradation of Proteins by Lysosomes
A. M. Cuervo (1996)
10.1083/JCB.110.6.1923
Studies on the mechanisms of autophagy: formation of the autophagic vacuole
W. Dunn (1990)
10.1247/CSF.20.465
Analysis of the membrane structures involved in autophagy in yeast by freeze-replica method.
M. Baba (1995)
10.1002/j.1460-2075.1995.tb00234.x
Yeast aminopeptidase I is post‐translationally sorted from the cytosol to the vacuole by a mechanism mediated by its bipartite N‐terminal extension.
B. Segui-Real (1995)
10.1111/J.1574-6968.1978.TB01876.X
Degradation of Peroxisomes after Transfer of Methanol-Grown Hansenula polymorpha into Glucose-Containing Media
M. Veenhuis (1978)
10.1016/S0092-8674(00)81707-9
Fab1p PtdIns(3)P 5-Kinase Function Essential for Protein Sorting in the Multivesicular Body
G. Odorizzi (1998)
A novel pathway of import of alpha-mannosidase, a marker enzyme of vacuolar membrane, in Saccharomyces cerevisiae.
T. Yoshihisa (1990)
10.1096/fasebj.11.14.9409540
Ubiquitin‐dependent internalization and down‐regulation of plasma membrane proteins
L. Hicke (1997)
10.1083/JCB.137.1.79
Endosome to Golgi Retrieval of the Vacuolar Protein Sorting Receptor, Vps10p, Requires the Function of the VPS29, VPS30, and VPS35 Gene Products
M. Seaman (1997)
10.1074/JBC.271.17.9934
Selective Uptake of Cytosolic, Peroxisomal, and Plasma Membrane Proteins into the Yeast Lysosome for Degradation (*)
H. L. Chiang (1996)
10.1083/JCB.132.6.999
Identification of a cytoplasm to vacuole targeting determinant in aminopeptidase I
M. N. Oda (1996)



This paper is referenced by
10.4161/auto.2.2.2366
Protein Aggregates are Transported to Vacuoles by Macroautophagic Mechanism in Nutrient-Starved Plant Cells
K. Toyooka (2006)
10.1126/SCIENCE.289.5479.563
All in the Ubiquitin Family
M. Hochstrasser (2000)
10.1104/PP.123.4.1227
Endoplasmic reticulum-derived compartments function in storage and as mediators of vacuolar remodeling via a new type of organelle, precursor protease vesicles.
M. Chrispeels (2000)
10.1091/MBC.12.12.3821
Cvt18/Gsa12 is required for cytoplasm-to-vacuole transport, pexophagy, and autophagy in Saccharomyces cerevisiae and Pichia pastoris.
J. Guan (2001)
10.1007/978-3-642-56373-7_3
Protein degradation in the aging organism.
W. Ward (2002)
10.1093/PCP/PCH031
3-methyladenine inhibits autophagy in tobacco culture cells under sucrose starvation conditions.
Chihiro Takatsuka (2004)
10.1016/S0070-2153(06)76003-3
Aggregate-prone proteins are cleared from the cytosol by autophagy: therapeutic implications.
A. Williams (2006)
10.1038/ncb1813
Midbody ring disposal by autophagy is a post-abscission event of cytokinesis
Christian Pohl (2009)
10.1021/cr800459r
ATG systems from the protein structural point of view.
N. N. Noda (2009)
10.1016/j.gene.2008.03.012
Identification and characterization of a splice variant of the catalytic domain of mouse NTE-related esterase.
Ping-an Chang (2008)
10.1039/c6pp00097e
Heat shock proteins in the context of photodynamic therapy: autophagy, apoptosis and immunogenic cell death.
M. Rodríguez (2016)
10.1074/jbc.C000307200
Structure of GATE-16, Membrane Transport Modulator and Mammalian Ortholog of Autophagocytosis Factor Aut7p*
Yakov Paz (2000)
10.1002/pmic.201700022
Proteomics Insights into Autophagy
Emmanuel K. Cudjoe (2017)
10.1038/cddis.2014.467
Regulation of autophagy by polyphenolic compounds as a potential therapeutic strategy for cancer
N. Hasima (2014)
10.1128/JB.183.20.5942-5955.2001
Aut5/Cvt17p, a putative lipase essential for disintegration of autophagic bodies inside the vacuole.
U. D. Epple (2001)
Review Autophagy in neurons: a review
K. Larsen (2002)
10.1038/nature01895
Nuclear cataract caused by a lack of DNA degradation in the mouse eye lens
S. Nishimoto (2003)
10.1074/JBC.M310793200
The Type 1 Phosphatase Reg1p-Glc7p Is Required for the Glucose-induced Degradation of Fructose-1,6-bisphosphatase in the Vacuole*
Dong-Ying Cui (2004)
10.4161/AUTO.1.1.1512
Trs85 is required for macroautophagy, pexophagy and cytoplasm to vacuole targeting in Yarrowia lipolytica and Saccharomyces cerevisiae.
T. Y. Nazarko (2005)
10.1161/01.RES.0000261924.76669.36
Distinct Roles of Autophagy in the Heart During Ischemia and Reperfusion: Roles of AMP-Activated Protein Kinase and Beclin 1 in Mediating Autophagy
Yutaka Matsui (2007)
10.1007/s00253-009-2271-6
ATP-citrate lyase activity and carotenoid production in batch cultures of Phaffia rhodozyma under nitrogen-limited and nonlimited conditions
Cipriano Chávez-Cabrera (2009)
10.1007/s00018-015-1917-z
Atg1 family kinases in autophagy initiation
N. N. Noda (2015)
The Molecular Mechanisms Underlying Fusion Events at the Lysosome Membrane
Mahmoud Abdul Karim (2017)
The Role of P62 in Autophagy of Salmonella Enterica Serovar Typhimurium
Yiyu Zheng (2011)
10.4161/auto.5.8.9996
Hypoxia-induced autophagy contributes to the chemoresistance of hepatocellular carcinoma cells
Jianrui Song (2009)
HLA-Ligandom- und Expressions-Analyse von Tumor- und Autophagie-assoziierten Antigenen in soliden Tumoren und Zelllinien
M. Müller (2008)
10.3390/ijms19061666
Wheat Gene TaATG8j Contributes to Stripe Rust Resistance
Md Abdullah-Al Mamun (2018)
10.1016/S0014-5793(02)03456-7
Yeast Mon1p/Aut12p functions in vacuolar fusion of autophagosomes and cvt‐vesicles
K. Meiling-Wesse (2002)
10.4161/auto.6308
Localization of autophagy-related proteins in yeast using a versatile plasmid-based resource of fluorescent protein fusions
J. Ma (2008)
10.1016/S0065-2296(02)38028-5
Golgi-independent trafficking of macromolecules to the plant vacuole
D. Bassham (2002)
10.1074/JBC.M300227200
α-Synuclein Is Degraded by Both Autophagy and the Proteasome*
J. Webb (2003)
10.1038/nature01860
Determining the position of the cell division plane
J. Canman (2003)
See more
Semantic Scholar Logo Some data provided by SemanticScholar