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Fluorescent Protein Biosensors: Measurement Of Molecular Dynamics In Living Cells.

K. Giuliano, P. Post, K. Hahn, D. L. Taylor
Published 1995 · Biology, Medicine

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A new generation of reagents that report on specific molecular events in living cells, called fluorescent protein biosensors, has evolved from in vitro fluorescence spectroscopy and fluorescent analogue cytochemistry. Creative designs of fluorescent protein biosensors to measure the molecular dynamics of macromolecules, metabolites, and ions in single cells emerge from the integrative use of contemporary synthetic organic chemistry, biochemistry, and molecular biology. Future advances in fluorescent probe design, computer-driven optical instrumentation, and software will allow us to engineer endogenous cellular components that localize and function as reporters of their activities, thus moving molecular measurement beyond the single cell to living tissues and the whole organism.
This paper references
Magnetic resonance microscopy of embryonic cell lineages and movements.
R. Jacobs (1994)
Calpain activity increases in hepatocytes following addition of ATP. Demonstration by a novel fluorescent approach.
B. Rosser (1993)
Fluores cent analogues: optical biosensors of the chemical and molecular dynamics of macromolecules in living cells. See Ref. 82a
K Hahn (1993)
Molec ­ ular cytochemistry : incorporation of fluorescently labeled actin into cells
DL Taylor (1978)
imer fluorescence of pyrene-labeled tropomyosin. A probe of conforma tional dynamics
Fluorescent analog cytochemistry: tracing functional protein components in living cells.
Y. Wang (1989)
Mobility of microinjected rho damine actin within living chicken gizzard cells determined by fluores cence photobleaching recovery
T E Kreis (1982)
Fluorescent analog cytochem ­ istry of contractile proteins
Wang Y-L (1982)
Membrane partition of fatty acids and inhibition of T cell function.
A. Anel (1993)
A genetically engineered , pro ­ tein - based optical biosensor of myosin II regulatory light chain phos ­ phorylation
PL Post (1994)
Incorporation of macromolecules into living cells.
P. Mcneil (1989)
Prob - ing the dynamic equilibrium of actin polymerization by fluorescence en ­ ergy transfer
Wang Y-L (1981)
Fluorescence - polari ­ zation spectrum and electronic - en ­ ergy transfer in proteins
G Weber (1960)
Cellular au­ tofluorescence-is it due to flavins? 1. Histochem
RC Benson (1979)
Why purify en zymes?
A Kornberg (1990)
Fluorescence spec ­ troscopy of proteins
L Stryer (1968)
Patterns of elevated free calcium and calmodulin activation in living cells
Klaus Hahn (1992)
1 98 1 . Detection of actin as ­ sembly by fluorescence energy trans
D Taylor
Confor ­ mational states of smooth muscle myosin . Effects of light chain phos ­ phorylation and ionic strength
KM Trybus (1984)
Fluorescent probes for analysis of cell structure, function, and health by flow and im aging cytometry. See Ref
A S Waggoner (1986)
Prepara ­ tion of a fluorescent analog : acetami ­ dofluoresceinyl - labeled Dictyostel ­ ium discoideum ) a - actinin
Simon (1986)
The submicroscopic properties of cytoplasm as a determinant of cellular function.
K. Luby-Phelps (1988)
Intracellular cyclic AMP not calcium, determines the direction of vesicle movement in melanophores: direct measurement by fluorescence ratio imaging
P. Sammak (1992)
The molecular dimen ­ sions and the monomer - dimer trans ­ formation of actin
TC Tsao (1953)
Fluo rescent glucagon derivatives. I. Sy n thesis and characterization of fluores cent glucagon derivatives
H Heithier (1988)
Fluorescence lifetime imaging.
J. R. Lakowicz (1992)
Chemical Re agents fo r Protein Modification
R L Lundblad (1991)
Pyruvate as a fluorescence quencher: a new spec­ troscopic assay for pyruvate
T Ando (1983)
A novel actin label: a fluorescent probe at glutamine-41 and its consequences.
R. Takashi (1988)
Practical applications and philosophy of optical spectroscopic probes.
G. Weber (1976)
Fluo rimetry study of N-(l-pyrenyl)io doacetamide-labelled F-actin . Local structural change of actin protomer ' both on polymerization and on bind ing of heavy meromyosin
T Kouyama (1981)
Aldol ­ ase exists in both the fluid and solid phases of cytoplasm
L Pagliaro (1988)
Binding and distribution of fluorescently labeled filamin in permeabilized and living cells.
B. Mittal (1987)
Fluorescently labelled molecules as probes of the structure and function of living cells
D. L. Taylor (1980)
Structure and function of actin.
W. Kabsch (1992)
Fluorescence po larization. See Ref
J R Lakowicz (1983)
Singlet energy transfer studies of the arrangement of proteins in the 30 S Escherichia coli ribosome.
K. Huang (1975)
Fluores ­ cence energy transfer in myosin sub ­ fragment - I
DJ Marsh (1980)
Detection of actin as sembly by fluorescence energy trans fer
D L Taylor (1981)
Fluorescent ana ­ log cytochemistry
DL Taylor (1984)
Rotational Brownian motion and polarization of the fluorescence of solutions.
G. Weber (1953)
Enzyme sub strates. See Ref
R P Haugland (1992)
Flu orescence studies of tubulin and mi crotubule dynamics in living cells. See Ref
E D Salmon (1986)
Dynamics and topographical distribution of surface glycoproteins during myoblast fusion: a resonance energy transfer study.
B. Herman (1982)
Behavior of a fluorescent ana ­ logue of calmodulin in living 3 T 3 cells
K Luby-Phelps (1985)
Resolution of structural changes associated with calcium activation of calmodulin using frequency domain fluorescence spectroscopy.
Y. Yao (1994)
Fluorescence energy transfer as a spectroscopic ruler.
L. Stryer (1978)
Polarization of the fluorescence of macromolecules. II. Fluorescent conjugates of ovalbumin and bovine serum albumin.
G. Weber (1952)
The new vision of light microscopy
D. L. Taylor (1992)
Fluorescent probes for analysis of cell structure , function , and health by flow and im ­ aging cytometry
AS Waggoner (1986)
Reso ­ nance energy transfer microscopy : observations of membrane - bound flu ­ orescent probes in model membranes and in living cells
PS Uster (1986)
Determinants of the translational mobility of a small solute in cell cytoplasm
H. P. Kao (1993)
Preparation of well-defined protein conjugates using enzyme-assisted reverse proteolysis.
K. Rose (1991)
Fluorescence an isotropy: theory and applications
R F Steiner (1991)
Synthe sis, spectral properties, and use of 6-acryloyl-2-dimethylaminonaphtha lene (acrylodan). 1. Bio i. Chern
F G Prendergast (1983)
Fluorescent probes in membrane s t u di e s
GK Radda (1975)
Enolase exists in the fluid phase of cytoplasm in 3T3 cells.
L. Pagliaro (1989)
Fluo ­ rescent indicators of adsorption in aqueous solution and on the solid phase
G Weber (1954)
A calcium-sensitive fluo rescent analog of calmodulin based on a novel calmodulin-binding fluoro phore
K M Hahn (1990)
Fluorescent probes for cytometry
AS Waggoner (1990)
See Ref. 66a, p. I
Biologically active fluorescent derivatives of spinach calmodulin that report calmodulin target protein binding.
J. Mills (1988)
Intra cellular measurement of oxygen by quenching of fluorescence of py renebutyric acid
J A Knopp (1972)
Gradients in the concentration and assembly of myosin II in living fibroblasts during locomotion and fiber transport.
J. Kolega (1993)
Effect of near-ultraviolet and visible light on mammalian cells in culture II. Formation of toxic photoproducts in tissue culture medium by blacklight.
J. Stoien (1974)
Spektroskopische Bestimmung des Dipolmomentes aromatischer Verbindungen im ersten angeregten Singulettzustand
E. Lippert (1957)
Autofluorescence of viable cultured mammalian cells.
J. Aubin (1979)
Spatial and temporal dynamics of specific molecules and ions in living cells. See Ref
D L Taylor (1986)
Charac ­ terization of skeletal muscle actin la ­ beled with the triplet probe erythro ­ sin - 5 - iodoacetamide
RD Ludescher (1993)
Excited states of pro teins
G Weber (1961)
A fluorescently labeled in ­ testinal fatty acid binding protein . In ­ teractions with fatty ac i d s and its use i n monitoring free fatty acids . 1
GV Richieri (1992)
Site-directed mutagenesis with an expanded genetic code.
D. Mendel (1995)
Oxygen quenching of pyrenebutyric acid fluorescence in water. A dynamic probe of the microenvironment.
W. M. Vaughan (1970)
Visualization of intermediate filaments in living cells using fluorescently labeled desmin.
B. Mittal (1989)
Fluorescent ana ­ logs of pep tides and hormones
FR Maxfield (1989)
Fluorescence and Phosphorescence of Proteins and Nucleic Acids
S. Konev (1967)
Fluorescence ratio imaging of cyclic AMP in single cells
S. Adams (1991)
A fluorescently labeled in testinal fatty acid binding protein
G V Richieri (1992)
Time - resolved fluorescence lifetime imaging
M vandeVen (1993)
T he interaction of a naphthalene dye with apomyoglobin and apohemoglobin . A fluorescent probe of non - polar binding sites . 1
L. Stryer (1965)
Resonance energy transfer microscopy.
B. Herman (1989)
Handbook of fluorescent probes and research chemicals
R. P. Haugland (1996)
S. Latt (1965)
Chemical modification of Cys‐374 of actin interferes with the formation of the profilactin complex
B. Malm (1984)
196 1 . Excited states of pro ­ teins
G Weber
Ca + - dependent phosphorylation of myosin light chain kinase decreases the Ca 2 + sen ­ sitivity of light chain phosphorylation within smooth muscle cells
MG Tansey (1994)
Formation and movement of myosin-containing structures in living fibroblasts
N. Mckenna (1989)
Flu ­ orescence studies of tubulin and mi ­ crotubule dynamics in living cells
ED Salmon (1986)
Principles of Fluorescence Sp ectroscopy
J R Lakowicz (1983)
Functional in ­ teractions between smooth muscle myosin light chain kinase and cal ­ modulin
DA Malencik (1982)
FRET Microscopy: Digital Imaging of Fluorescence Resonance Energy Transfer. Application in Cell Biology
T. Jovin (1989)
Rapid kinetic studies on calcium interactions with native and fluorescently labeled calmodulin.
D. Malencik (1981)
Fluorescence lifetime imaging of free and protein-bound NADH.
J. R. Lakowicz (1992)
Engineering firefly luciferase as an indicator of cyclic AMP‐dependent protein kinase in living cells
G. Sala-Newby (1992)
Involvement of pro ­ filin in the actin - based motility of L . monocytogenes in cells and in cell ­ free extracts
Theriot lA (1994)
Facile synthesis ofho ­ mogeneous artificial proteins . 1
K Rose (1994)
Behav ­ ior of cells in tissue cultures
WH Lewis (1924)
Mi croinjections of fluorescently labeled proteins into living cells with empha sis on cytoskeletal proteins
T E Kreis (1982)
Specific acylation of calmodulin. Synthesis and adduct formation with a fluorenyl-based spin label.
A. E. Jackson (1984)
Energy transfer: a spectroscopic ruler.
L. Stryer (1967)
Quantitation of fluorescence energy transfer between cell surface proteins via fluorescence donor photobleaching kinetics.
R. Young (1994)
Spatial and temporal dynamics of specific molecules and ions in living cells
DL Taylor (1986)
Resonance en ­ ergy transfer : methods and applica ­ tions
P Wu (1994)
Hindered diffu ­ sion of inert tracer particles in the cy ­ toplasm of mouse 3 T 3 cells
K Luby-Phelps (1987)
Location of chromophoric residues in proteins by solvent perturbation. I. Tyrosyls in serum albumins.
T. Herskovits (1962)
Regulation of isocitrate dehydrogenase by phosphorylation involves no long-range conformational change in the free enzyme.
J. Hurley (1990)
Ribosome-mediated incorporation of a non-standard amino acid into a peptide through expansion of the genetic code
J. D. Bain (1992)
The selective quenching of tryptophan fluorescence in proteins by iodide ion: lysozyme in the presence and absence of substrate.
S. Lehrer (1967)
Spin-labeled biomolecules.
T. Stone (1965)

This paper is referenced by
Genetic Engineering of Signaling Molecules
A. Feltus (2002)
Long-term multiple color imaging of live cells using quantum dot bioconjugates
Jyoti K. Jaiswal (2003)
Cell‐Cycle Markers and Biosensors
Laetitia Kurzawa (2010)
The phytofluors: a new class of fluorescent protein probes
J. Murphy (1997)
Topographical and Physicochemical Modification of Material Surface to Enable Patterning of Living Cells
D. Jung (2001)
Optical determination of glutamine using a genetically engineered protein.
J. Dattelbaum (2001)
Multiplexed high content screening assays create a systems cell biology approach to drug discovery.
D. Taylor (2005)
High Content Screening
John M. Walker (2006)
Semi-continuous, label-free immunosensing approach for Ca2+-based conformation change of a calcium-binding protein.
Sung-Ho Paek (2014)
Visualizing and quantifying adhesive signals.
Mohsen Sabouri-Ghomi (2008)
Cellular probes on the move
S. Simon (1996)
Systeme destine a un criblage a base de cellules
R. Rubin (1999)
Measurement and manipulation of cytoskeletal dynamics in living cells.
K. Giuliano (1995)
Systeme de criblage pour cellules
G. Bright (1999)
Fluorescent tags of protein function in living cells.
M. Whitaker (2000)
Automated Light Microscopy for the Study of the Brain: Cellular and Molecular Dynamics, Development, and Tumorigenesis a
D. L. Taylor (1997)
The D evelopment of Optical Nanosensor Technology for Single Cell Analysis
P. Kasili (2004)
Fluorescent proteins and engineered cell lines.
N. Thomas (2007)
Development of fluorescent biosensors for probing the function of motor proteins.
M. Webb (2007)
Transition from classical methods to new strategies: Mechanistic evaluation of inhibitors against Mycobacterium tuberculosis shikimate kinase
Ngolui René Fuanta (2018)
Characterization of Cells, Microscopic
E. Huebner (2003)
3-Hydroxybenzo[g]quinolones: dyes with red-shifted absorption and highly resolved dual emission
Mykhailo D. Bilokin (2009)
Modularity: a new perspective in biology.
D. N. Dani (2007)
Fluorescence-based biosensors.
M. Strianese (2012)
Fluorescent protein biosensors applied to microphysiological systems
Nina Senutovitch (2015)
S. Gilroy (1997)
Dual-Fluorescence Probe of Environment Basicity (Hydrogen Bond Accepting Ability) Displaying no Sensitivity to Polarity
Mykhailo D. Bilokin (2008)
Luminescent gold nanoparticles: A new class of nanoprobes for biomedical imaging
Chen Zhou (2013)
Application of cyclic biamperometry to viability and cytotoxicity assessment in human corneal epithelial cells
Mehdi Rahimi (2013)
Split-Inteins for Simultaneous, site-specific conjugation of Quantum Dots to multiple protein targets In vivo
A. Charalambous (2011)
Fluorescence-based Biosensors
Michael Schäferling (2011)
Herencia Y Adaptación (1999)
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