Online citations, reference lists, and bibliographies.
← Back to Search

MR Imaging Of Phagocytosis In Experimental Gliomas.

C. Zimmer, R. Weissleder, K. Poss, A. Bogdanova, S. C. Wright, W. S. Enochs
Published 1995 · Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
PURPOSE To determine whether phagocytosis can be observed in vivo in glioma cells. MATERIALS AND METHODS Rat C6 glioma cells were studied in culture and after intracerebral implantation into 13 rats. Monocrystalline iron oxide nanoparticles (MION), a model marker of phagocytosis, was administered intravenously to tumor-bearing rats at 2-20 mg of iron per kilogram. Magnetic resonance (MR) imaging was performed at multiple time points. RESULTS Glioma cells in culture showed uptake of MION in amounts of up to 10 ng of iron per 10(6) cells, corresponding to approximately 50,000 particles per cell. Fluorescently labeled MION was found to be located primarily in tubular lysosomes. Intracerebral gliomas showed characteristic changes in signal intensity at MR imaging that peaked 12 hours after administration of MION and lasted up to 5 days; these changes corresponded to uptake and subsequent biodegradation of MION by tumor cells. CONCLUSION Phagocytosis of glioma cells can be detected in vivo with iron oxide-enhanced MR imaging, and this may permit accurate delineation of tumor margins.



This paper is referenced by
10.1016/J.BIOMATERIALS.2006.05.047
Inactivation of nanocrystalline C60 cytotoxicity by γ-irradiation
A. Isakovic (2006)
Comparison of two superparamagnetic viral-sized iron oxide particles ferumoxides and ferumoxtran-10 with a gadolinium chelate in imaging intracranial tumors.
P. Várallyay (2002)
10.1016/j.nano.2009.10.001
Tumor-associated macrophages are predominant carriers of cyclodextrin-based nanoparticles into gliomas.
D. Alizadeh (2010)
10.1007/978-1-4757-6482-6_40
Magnetic Nanoparticles as Contrast Agents for MR Imaging
J. Bulte (1997)
10.1016/j.nurt.2007.05.005
Magnetic resonance imaging of human brain macrophage infiltration
K. Petry (2011)
10.1111/j.1751-1097.2010.00742.x
Novel Photodynamic Therapy Using Water‐dispersed TiO2–Polyethylene Glycol Compound: Evaluation of Antitumor Effect on Glioma Cells and Spheroids In Vitro
S. Yamaguchi (2010)
10.1007/978-1-4614-5915-6_12
Imaging angiogenesis, inflammation, and metastasis in the tumor microenvironment with magnetic resonance imaging.
S. Serres (2014)
10.1007/s00062-003-4347-5
Advances in Brain Tumor Imaging
O. Jansen (2003)
10.1002/jmri.20283
Differential uptake of ferumoxtran‐10 and ferumoxytol, ultrasmall superparamagnetic iron oxide contrast agents in rabbit: Critical determinants of atherosclerotic plaque labeling
A. D. Yancy (2005)
10.1023/A:1006285800794
Sequential Imaging and Volumetric Analysis of an Intracerebral C6 Glioma by Means of a Clinical MRI System
F. Raila (2004)
10.1039/B712795B
Monodisperse water-soluble magnetite nanoparticles prepared by polyol process for high-performance magnetic resonance imaging.
J. Wan (2007)
10.1080/01616412.2000.11740705
Laser-induced fluorescence detection of malignant gliomas using fluorescein-labeled serum albumin: Experimental and preliminary clinical results
P. Kremer (2000)
10.1007/978-1-4614-7876-8_19
Targeting Drugs to Cancer: A Tough Journey to the Tumor Cell
Shiran Ferber (2013)
10.1016/j.ejrad.2009.01.042
Nanomedicine: perspective and promises with ligand-directed molecular imaging.
D. Pan (2009)
10.1504/IJBNN.2010.034651
Synthesis, surface architecture and biological response of superparamagnetic iron oxide nanoparticles for application in drug delivery: a review
M. Mahmoudi (2010)
10.1007/s11307-015-0874-0
MRI of High-Glucose Metabolism Tumors: a Study in Cells and Mice with 2-DG-Modified Superparamagnetic Iron Oxide Nanoparticles
X. H. Shan (2015)
10.1148/radiol.2481071260
Macrophage activity in infected areas of an experimental vertebral osteomyelitis model: USPIO-enhanced MR imaging--feasibility study.
G. Bierry (2008)
10.1016/j.biomaterials.2011.10.077
ICP-MS analysis of lanthanide-doped nanoparticles as a non-radiative, multiplex approach to quantify biodistribution and blood clearance.
S. Crayton (2012)
10.1007/978-1-4614-5915-6
Tumor Microenvironment and Cellular Stress
Constantinos Koumenis (2014)
10.1007/s001170050446
Verbessern superparamagnetische Kontrastmittel die MR- tomographische Abgrenzbarkeit experimenteller Gliome?
T. Egelhof (1998)
Investigation of tumour characteristics and treatment strategies in animal models using multiparametric MRI and novel contrast agents
C. B. Rygh (2007)
10.1007/978-3-642-58731-3_39
Intraoperative Diagnostic and Interventional MRI in Neurosurgery: First Experience with an “Open MR” System
F. Albert (1998)
10.1002/ijc.20048
Chemotherapy of glioblastoma in rats using doxorubicin‐loaded nanoparticles
S. C. Steiniger (2004)
10.1148/RADIOLOGY.214.2.R00FE19568
Tumoral distribution of long-circulating dextran-coated iron oxide nanoparticles in a rodent model.
A. Moore (2000)
10.1016/S0304-8853(00)01264-6
Glial brain tumor targeting of magnetite nanoparticles in rats
O. Mykhaylyk (2001)
10.1162/15353500200303163
A Novel Polyacrylamide Magnetic Nanoparticle Contrast Agent for Molecular Imaging using MRI
B. Moffat (2003)
10.1002/NBM.923
Applications of ultrasmall superparamagnetic iron oxide contrast agents in the MR study of animal models
E. Wu (2004)
10.1007/978-3-642-56662-2_6
Recent Advances in MR Contrast Media
V. Dousset (2001)
10.1161/ATVBAHA.109.198812
Pharmacological Inhibition of C-C Chemokine Receptor 2 Decreases Macrophage Infiltration in the Aortic Root of the Human C-C Chemokine Receptor 2/Apolipoprotein E−/− Mouse: Magnetic Resonance Imaging Assessment
A. Olzinski (2010)
10.4172/2155-9899.1000226
Imaging Neuroinflammation – from Bench to Bedside
B. Pulli (2014)
10.1201/B15274-7
Endohedral Metallofullerenes, Iron Oxide Agents, and Gold Nanoparticles for Brain Imaging
M. McAteer (2013)
10.1002/JMRI.1880070629
Uptake of dextran‐coated monocrystalline iron oxides in tumor cells and macrophages
A. Moore (1997)
See more
Semantic Scholar Logo Some data provided by SemanticScholar