← Back to Search
Gender Dimorphism In Rat Liver Mitochondrial Oxidative Metabolism And Biogenesis.
R. Justo, J. Boada, Margalida Frontera, J. Oliver, Jordi Bermúdez, M. Gianotti
Published 2005 · Medicine, Biology
Download PDFAnalyze on Scholarcy
In the present study, we have investigated gender differences in rat liver mitochondrial oxidative metabolism. Total mitochondrial population (M) as well as the heavy (M1), medium (M3), and light (M8) mitochondrial fractions obtained by means of differential centrifugation steps at 1,000, 3,000, and 8,000 g, respectively, were isolated. Electron microscopic analysis was performed and mitochondrial protein content and cardiolipin levels, mitochondrial O(2) flux, ATP synthase activity, mitochondrial membrane potential, and mitochondrial transcription factor A (TFAM) protein levels were measured in each sample. Our results indicate that mitochondria from females have higher protein content and higher cardiolipin levels, greater respiratory and phosphorylative capacities, and more-energized mitochondria in respiratory state 3. Moreover, protein levels of TFAM were four times greater in females than in males. Gender differences in the aforementioned parameters were more patent in the isolated heavy M1 and M3 mitochondrial fractions. The present study demonstrates that gender-related differences in liver mitochondrial function are due mainly to a higher capacity and efficiency of substrate oxidation, likely related to greater mitochondrial machinery in females than in males, which is in accord with greater mitochondrial differentiation in females.
This paper references
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
Mitochondria as a Pharmacological Target
A. Szewczyk (2002)
Hepatotoxicity due to mitochondrial dysfunction
D. Pessayre (2004)
Structural and Functional Changes in Mitochondria Associated with Trophoblast Differentiation: Methods to Isolate Enriched Preparations of Syncytiotrophoblast Mitochondria.
Federico Marti Nez (1997)
Specific measurement of DNA in nuclei and nucleic acids using diaminobenzoic acid.
P. Thomas (1978)
Import of mitochondrial transcription factor A (TFAM) into rat liver mitochondria stimulates transcription of mitochondrial DNA.
H. L. Garstka (2003)
Restriction of energy intake, energy expenditure, and aging.
J. Ramsey (2000)
Changing Patterns of Transcriptional and Post-transcriptional Control of β-F1-ATPase Gene Expression during Mitochondrial Biogenesis in Liver (*)
J. Izquierdo (1995)
Body mass dependence of H+ leak in mitochondria and its relevance to metabolic rate
R. K. Porter (1993)
Thyroid hormones regulate the onset of osmotic activity of rat liver mitochondria after birth.
A. Almeida (1997)
Cold exposure induces different uncoupling-protein thermogenin masking/unmasking processes in brown adipose tissue depending on mitochondrial subtypes.
M. Moreno (1994)
Am J Physiol
Light mitochondria and cellular thermogenesis.
F. Goglia (1988)
Transient activation of mitochondrial translation regulates the expression of the mitochondrial genome during mammalian mitochondrial differentiation.
L. K. Ostronoff (1996)
Mitochondrial transcription factors B1 and B2 activate transcription of human mtDNA
M. Falkenberg (2002)
Sex-dependent dietary obesity, induction of UCPs, and leptin expression in rat adipose tissues.
A. Rodríguez (2001)
The effect of thyroid state on respiratory activities of three rat liver mitochondrial fractions
F. Goglia (1989)
Electrical stimulation of neonatal cardiomyocytes results in the sequential activation of nuclear genes governing mitochondrial proliferation and differentiation.
Y. Xia (1997)
Brown adipose tissue mitochondrial subpopulations show different morphological and thermogenic characteristics.
R. Justo (2005)
High phosphorylation efficiency and depression of uncoupled respiration in mitochondria under hypoxia.
E. Gnaiger (2000)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
U. Laemmli (1970)
Why do cancers have high aerobic glycolysis?
R. Gatenby (2004)
Thyroid function and aging: genderrelated differences
VM Da Costa (2001)
Mitochondrial form and function relationships in vivo: their potential in toxicology and pathology.
R. Smith (1983)
Properties of porcine white adipose tissue and liver mitochondrial subpopulations.
T. Koekemoer (2001)
Mitochondrial membrane potential and aging
D. Nicholls (2004)
Repeated dose 28-day oral toxicity study in Wistar rats with a mixture of five pesticides often found as residues in food: alphacypermethrin, bromopropylate, carbendazim, chlorpyrifos and mancozeb.
Hélène Jacobsen (2004)
Mitochondria from females exhibit higher antioxidant gene expression and lower oxidative damage than males.
C. Borrás (2003)
Sex-associated differences in cold-induced UCP1 synthesis in rodent brown adipose tissue
S. Quevedo (1998)
Subfractionation of mitochondria and isolation of the proteins of oxidative phosphorylation. In: Mitochondria: A practical approach
Ci Ragan (1987)
Biochem J 348 Pt
Nitric oxide, complex I, and the modulation of mitochondrial reactive species in biology and disease.
M. Carreras (2004)
Mitochondrial Damage in Aging and Apoptosis
J. Sastre (2002)
Gender-related differences in morphology and thermogenic capacity of brown adipose tissue mitochondrial subpopulations.
R. Justo (2005)
Mechanisms Controlling Mitochondrial Biogenesis and Respiration through the Thermogenic Coactivator PGC-1
Z. Wu (1999)
Hypothyroidism in rats does not lower mitochondrial ADP/O and H+/O ratios.
R. Hafner (1988)
Subacute (28-day) toxicity of furfural in Fischer 344 rats: a comparison of the oral and inhalation route.
J. Arts (2004)
Transient overexpression of mitochondrial transcription factor A (TFAM) is sufficient to stimulate mitochondrial DNA transcription, but not sufficient to increase mtDNA copy number in cultured cells.
K. Maniura-Weber (2004)
Estrogen's Effects on Mitochondrial Gene Expression: Mechanisms and Potential Contributions to Estrogen Carcinogenesis
Jin-qiang Chen (2004)
Mitochondrial biogenesis and development of respiratory chain enzymes in kidney cells: role of glucocorticoids.
F. Djouadi (1994)
Changing patterns of transcriptional and post-transcriptional control of beta-F1-ATPase gene expression during mitochondrial biogenesis in liver preparations of syncytiotrophoblast mitochondria
Jm Izquierdo (1997)
Measurement of Mitochondrial Membrane Potential Using Fluorescent Rhodamine Derivatives
R. Scadutojr (1999)
Structural and functional changes in mitochondria associated with trophoblast differentiation: methods to isolate enriched preparations of syncytiotrophoblast mitochondria.
F. Martínez (1997)
Subfractionation of mitochondria and isolation of the proteins of oxidative phosphorylation
CI Ragan (1987)
Mitochondria: a practical approach.
V. Darley-Usmar (1987)
Effect of 12, 24 and 72 hours fasting in thermogenic parameters of rat brown adipose tissue mitochondrial subpopulations.
M. Gianotti (1998)
Sex-dependent Thermogenesis, Differences in Mitochondrial Morphology and Function, and Adrenergic Response in Brown Adipose Tissue*
S. Rodríguez-Cuenca (2002)
Perinatal maturation of rat kidney mitochondria.
B Prieur (1995)
Thyroid function and aging: gender-related differences.
V. M. D. da Costa (2001)
Sexual dimorphism in the adrenergic control of rat brown adipose tissue response to overfeeding
E. Rodríguez (2001)
Evidence that metformin exerts its anti-diabetic effects through inhibition of complex 1 of the mitochondrial respiratory chain.
Marc R. Owen (2000)
Similarity of human mitochondrial transcription factor 1 to high mobility group proteins.
M. Parisi (1991)
AC Tose (1993)
Morphofunctional changes in the mitochondrial subpopulations of conceptus tissues during the placentation process
R. Justo (2002)
Allometry of mammalian cellular oxygen consumption
R. K. Porter (2001)
Effects of adrenal steroid hormones on mitochondrial maturation during the late fetal period.
B Prieur (1998)
Replication and Transcription of Mammalian Mitochondrial Dna
P. Fernández-Silva (2003)
Cellular oxygen consumption depends on body mass.
R. K. Porter (1995)
Evolution of energy metabolism. Proton permeability of the inner membrane of liver mitochondria is greater in a mammal than in a reptile.
M. Brand (1991)
Evidence for masking of brown adipose tissue mitochondrial GDP-binding sites in response to fasting in rats made obese by dietary manipulation. Effects of reversion to standard diet.
P. Puigserver (1991)
Biochemical and functional differences in rat liver mitochondrial subpopulations obtained at different gravitational forces.
A. Lanni (1996)
Brown adipose tissue response to cafeteria diet-feeding involves induction of the UCP2 gene and is impaired in female rats as compared to males
P. Roca (1999)
The biosynthesis and functional role of cardiolipin.
M. Schlame (2000)
Mitochondrial function and dysfunction in the cell: its relevance to aging and aging-related disease.
D. Nicholls (2002)
This paper is referenced by
Impaired mitochondrial oxidative phosphorylation in the peroxisomal disease X-linked adrenoleukodystrophy.
J. López-Erauskin (2013)
Gender Dimorphism in High-Fat-Diet-Induced Insulin Resistance in Skeletal Muscle of Aged Rats
Yolanda Gómez-Pérez (2008)
Mitochondrial Physiology in the Major Arbovirus Vector Aedes aegypti: Substrate Preferences and Sexual Differences Define Respiratory Capacity and Superoxide Production
J. C. Soares (2015)
Gender-dimorphic regulation of liver proteins in Streptozotocin-induced diabetic rats
H. Chaudhari (2014)
Comparative hepatic proteome analysis between lean and obese rats fed a high‐fat diet reveals the existence of gender differences
X. Wang (2012)
Gender-dimorphic regulation of antioxidant proteins in response to high-fat diet and sex steroid hormones in rats
H. Chaudhari (2014)
Gender-based differences in mechanisms of protection in myocardial ischemia-reperfusion injury.
E. Murphy (2007)
Long-term high-fat-diet feeding induces skeletal muscle mitochondrial biogenesis in rats in a sex-dependent and muscle-type specific manner
Yolanda Gómez-Pérez (2012)
Gender specific differences in the liver proteome of rats exposed to short term and low-concentration hexabromocyclododecane (HBCD)
C. Diepenbroek (2016)
Modeling insulin resistance in rodents by alterations in diet: what have high-fat and high-calorie diets revealed?
Lewin Small (2018)
The role of telomerase in brain during ageing and under dietary restriction
Rafal Czapiewski (2013)
Tissue and sex specificities in Ca2+ handling by isolated mitochondria in conditions avoiding the permeability transition
Hanan Chweih (2015)
Male mice that lack the G-protein-coupled receptor GPR41 have low energy expenditure and increased body fat content.
M. Bellahcene (2013)
A long-term study on female mice fed on a genetically modi W ed soybean : e V ects on liver ageing
M. Malatesta (2008)
Estrogen receptor beta interacts and colocalizes with HADHB in mitochondria.
Zhenqi Zhou (2012)
Gender Difference in Proteome of Brown Adipose Tissues between Male and Female Rats Exposed to a High Fat Diet
Duk Kwon Choi (2011)
Cardioprotection in females: a role for nitric oxide and altered gene expression
E. Murphy (2007)
Skeletal Muscle of Female Rats Exhibit Higher Mitochondrial Mass and Oxidative-Phosphorylative Capacities Compared to Males
B. Colom (2007)
Early and gender-specific differences in spinal cord mitochondrial function and oxidative stress markers in a mouse model of ALS
D. Cacabelos (2016)
Modulation of oxidative phosphorylation machinery signifies a prime mode of anti-ageing mechanism of calorie restriction in male rat liver mitochondria
D. Dani (2009)
The role of sex in cardiac function and disease.
M. Czubryt (2006)
Estrogen and the regulation of mitochondrial structure and function in the brain
S. Arnold (2012)
Sexual Dimorphism in the Alterations of Cardiac Muscle Mitochondrial Bioenergetics Associated to the Ageing Process.
B. Colom (2015)
Peculiarities of hormonal metabolic changes in adult male rats under effect of emotional stress induced at a time of newborn suckling
Velychko Nf (2014)
Hepatocellular carcinoma: the rising tide from east to west—a review of epidemiology, screening and tumor markers
H. Monsour (2013)
Effect of caloric restriction on oxidative markers.
J. Škrha (2009)
Mitochondrial oxidative stress in female and male rat brain after ex vivo carbon monoxide treatment
D. Taşkıran (2007)
Gender-dependent Metabolic Remodeling During Heart Preservation in Cardioplegic Celsior and Histidine Buffer Solution
M. Alves (2012)
17β-estradiol improves hepatic mitochondrial biogenesis and function through PGC1B.
Bel M. Galmés-Pascual (2017)
Gastrocnemius mitochondrial respiration: are there any differences between men and women?
J. Thompson (2013)
High-fat diet feeding induces a depot-dependent response on the pro-inflammatory state and mitochondrial function of gonadal white adipose tissue.
E. Amengual-Cladera (2013)
Gender-dimorphic regulation of DJ1 and its interactions with metabolic proteins in streptozotocin-induced diabetic rats
H. Chaudhari (2015)See more