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Biphasic Regulation Of Paracellular Permeability In Human Cervical Cells By Two Distinct Nucleotide Receptors.

G. Gorodeski, U. Hopfer, B. De Santis, R. Eckert, E. Rorke, W. Utian
Published 1995 · Biology, Medicine

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The effects of extracellular ATP (ATPe) were characterized in human cervical cells grown on filters. ATPe changed the transepithelial conductance (GT) in a biphasic manner with an initial acute increase of 13 +/- 3% (phase I), lasting approximately 2 min, followed by a sustained decrease of 49 +/- 17% (phase II). ATPe also effected decreases of permeabilities to pyranine and to sucrose, which correspond to the phase II decrease in GT. Both phase I and II effects appear to be mediated by apical nucleotide receptors. However, the phase I effect differed from the phase II effect as follows: 1) a higher 50% effective concentration for ATPe, 22 vs. 3 microM; 2) different nucleotide specificity; 3) lack of influence of pretreatment with pertussis toxin; 4) independence from time in culture after seeding; and 5) involvement of cytosolic calcium, rather than diacylglycerol, as intracellular messenger. These differences suggest the presence of two different types of nucleotide receptors that use different intracellular signaling systems and have opposite effects on the paracellular permeability and electrical conductance. These results suggest that ATPe could regulate cervical mucus production in vivo by regulating the paracellular permeability. Depending on the specific receptors present, ATPe could either increase or decrease mucus production.
This paper references
10.1111/j.1476-5381.1993.tb12862.x
The regulation of aortic endothelial cells by purines and pyrimidines involves co‐existing P2y‐purinoceptors and nucleotide receptors linked to phospholipase C
G. Wilkinson (1993)
10.1042/BJ2630371
Extracellular nucleotides stimulate receptor-mediated calcium mobilization and inositol phosphate production in human fibroblasts.
J. Fine (1989)
10.1016/0076-6879(88)57093-3
Computer programs for calculating total from specified free or free from specified total ionic concentrations in aqueous solutions containing multiple metals and ligands.
A. Fabiato (1988)
10.1113/jphysiol.1984.sp015385
Source and concentration of extracellular adenosine triphosphate during haemostasis in rats, rabbits and man.
G. Born (1984)
10.1002/JCP.1041560214
Platelet‐activating factor stimulates phosphatidic acid formation in cultured rat mesangial cells: Roles of phospholipase D, diglyceride kinase, and de novo phospholipid synthesis
M. Kester (1993)
10.1016/0006-291X(89)91517-9
Two distinct receptors for ATP can be distinguished in Swiss 3T6 mouse fibroblasts by their desensitization.
F. González (1989)
10.1002/JCP.1041540322
Intracellular signalling by nucleotide receptors in PC12 pheochromocytoma cells
S. Raha (1993)
10.1046/J.1432-0436.1994.56120107.X
Human uterine cervical epithelial cells grown on permeable support--a new model for the study of differentiation.
G. Gorodeski (1994)
10.1042/BJ2840733
A nucleotide receptor in vascular endothelial cells is specifically activated by the fully ionized forms of ATP and UTP.
K. Lustig (1992)
10.1016/0968-0004(92)90007-V
Crosstalk among multiple signal-activated phospholipases.
M. Liscovitch (1992)
10.1113/jphysiol.1975.sp010925
On the association between transmitter secretion and the release of adenine nucleotides from mammalian motor nerve terminals.
E. M. Silinsky (1975)
10.1177/107155769400100309
Characterization of Paracellular Permeability in Cultured Human Cervical Epithelium
G. Gorodeski (1994)
10.1111/J.1471-4159.1993.TB03262.X
Signal Transduction Pathways Coupled to a P2U Receptor in Neuroblastoma × Glioma (NG108‐15) Cells
Tai-an Lin (1993)
10.1073/PNAS.90.11.5113
Expression cloning of an ATP receptor from mouse neuroblastoma cells.
K. Lustig (1993)
10.1042/BJ2930649
Correlation between secretion and phospholipase D activation in differentiated HL60 cells.
J. Stutchfield (1993)



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10.1152/AJPCELL.2002.282.1.C75
Regulation of transcervical permeability by two distinct P2 purinergic receptor mechanisms.
G. Gorodeski (2002)
10.1269/JRR.44.15
Oxygen delivery in irradiated normal tissue.
M. Kiani (2003)
10.1152/AJPCELL.2002.282.1.C84
Expression, regulation, and function of P2X(4) purinergic receptor in human cervical epithelial cells.
G. Gorodeski (2002)
10.1016/J.NUT.2005.03.003
Dietary nucleotides and human immune cells. II. Modulation of PBMC growth and cytokine secretion.
E. Holen (2006)
10.1007/BF02737899
Purinergic receptor-induced changes in paracellular resistance across cultures of human cervical cells are mediated by two distinct cytosolic calcium-related mechanisms
G. Gorodeski (2007)
10.1007/s00424-005-1388-4
Regulation of ion transport via apical purinergic receptors in intact rabbit airway epithelium
A. N. Poulsen (2005)
10.1002/CM.10156
PLA2 and secondary metabolites of arachidonic acid control filopodial behavior in neuronal growth cones.
Matthew S. Geddis (2004)
10.1007/978-0-387-74901-3_15
Regulation of Paracellular Permeability in Low-Resistance Human Vaginal-Cervical Epithelia
G. Gorodeski (2008)
10.3109/10799899809047748
Receptor-operated calcium influx mediated by protein tyrosine kinase pathways.
Y. Tsunoda (1998)
10.1152/ajpcell.1998.274.4.C1108
Retinoids modulate P2U purinergic receptor-mediated changes in transcervical paracellular permeability.
G. Gorodeski (1998)
10.1016/S0015-0282(97)00471-8
Seminal fluid factor increases the resistance of the tight junctional complex of cultured human cervical epithelium CaSki cells.
G. Gorodeski (1998)
10.1002/9781119266136.CH8
Anatomy and Physiology of Arousal
K. Park (2018)
10.1074/JBC.275.5.3667
Prolactin-releasing Peptide Activation of the Prolactin Promoter Is Differentially Mediated by Extracellular Signal-regulated Protein Kinase and c-Jun N-terminal Protein Kinase*
A. Kimura (2000)
10.1152/ajpcell.1998.274.4.C1108
Retinoids modulate P2Upurinergic receptor-mediated changes in transcervical paracellular permeability.
G. Gorodeski (1998)
10.1080/10284150600627128
Relationship between fatty acids and the endocrine and neuroendocrine system
S. Bhathena (2006)
10.1007/978-1-4615-5399-1_47
Skeletal muscle function, oxygenation and biochemistry in an endotoxemic model of SIRS.
E. Iannoli (1997)
10.1016/S1071-5576(96)00028-7
The Cultured Human Cervical Epithelium: A New Model for Studying Paracellular Transport
G. Gorodeski (1996)
10.1152/ajpcell.1999.277.2.C202
Focus on "Multiple functional P2X and P2Y receptors in the luminal and basolateral membranes of pancreatic duct cells".
G. Dubyak (1999)
10.1152/ajpcell.1998.275.3.C758
Regulation by retinoids of P2Y2 nucleotide receptor mRNA in human uterine cervical cells.
G. Gorodeski (1998)
10.1016/S0015-0282(02)04677-0
Selective P2Y2 receptor agonists stimulate vaginal moisture in ovariectomized rabbits.
K. Min (2003)
10.1007/s11302-009-9161-3
P2X7 receptor expression is decreased in epithelial cancer cells of ectodermal, uro-genital sinus, and distal paramesonephric duct origin
X. Li (2009)
10.1163/156856209X444330
In Vivo Validation of Biological Responses of bFGF Released from Microspheres Formulated by Blending Poly-Lactide-co-Glycolide and Poly(ethylene glycol)-Grafted-Chitosan in Hamster Cheek Pouch Microcirculatory Models
Christine A. Falabella (2009)
10.1210/JCEM.85.7.6671
Effects of menopause and estrogen on cervical epithelial permeability.
G. Gorodeski (2000)
10.1177/107155769600300507
The Cultured Human Cervical Epithelium: A New Model for Studying Paracellular Transport
G. Gorodeski (1996)
10.1007/s004419900138
Distribution of P2X receptor subtypes in the rat female reproductive tract at late pro-oestrus/early oestrus
M. Bardini (1999)
10.1111/j.1743-6109.2010.01903.x
Biochemical factors modulating female genital sexual arousal physiology.
A. Traish (2010)
10.1007/s11302-020-09693-3
Purinergic signaling and tumor microenvironment in cervical Cancer
Marta Schmidt Pfaffenzeller (2020)
10.1007/978-1-4615-0205-0_58
Simulations of capillary network oxygen transport during transient ischemia in the presence and absence of tissue myoglobin.
D. Goldman (2003)
10.1152/ajpcell.1999.277.2.C202
Focus on "multiple functional P2X and P2Y receptors in the luminal and basolateral membranes of pancreatic duct cells".
G. Dubyak (1999)
10.1016/S1071-5576(98)00013-6
Modulation of coronary vascular resistance in female rabbits by estrogen and progesterone.
G. Gorodeski (1998)
10.1016/j.autneu.2015.04.008
Purinergic Signalling in the Reproductive System
G. Gorodeski (2015)
10.1046/j.1440-1681.2000.03340.x
Role For Capillaries In Coupling Blood Flow With Metabolism
I. Sarelius (2000)
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