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Gp60 Is An Albumin-binding Glycoprotein Expressed By Continuous Endothelium Involved In Albumin Transcytosis.

J. Schnitzer
Published 1992 · Biology, Medicine

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Albumin reduces capillary permeability and acts as a carrier for various small molecules. Recently, we identified a 60-kDa sialoglycoprotein (gp60) on the surface of cultured rat microvascular endothelial cells (MEC) that binds albumin and antiglycophorin serum (alpha-gp). We verified that alpha-gp recognizes the albumin-binding gp60 by affinity, purifying proteins from MEC extracts using immobilized albumin. gp60 was immunoblotted with alpha-gp only when the MEC extract was reacted with albumin and not in controls. We immunoprecipitated gp60 from biosynthetically radiolabeled MEC lysates and from extracts containing endothelial surface proteins of isolated rat hearts that were radioiodinated in situ. gp60 was immunoblotted selectively in rat tissue microvascular beds lined with continuous endothelium (heart, lung, diaphragm, fat, skeletal muscle, mesentery, and duodenal muscularis but not cortical brain) and not those exclusively lined with fenestrated or sinusoidal endothelium (adrenal, pancreas, liver, and small intestinal mucosa). MEC isolated from rat heart, lung, and epididymal fat pad expressed gp60 and bound albumin, whereas various nonendothelial cells and brain-derived MEC did not. gp60 is an albumin-binding glycoprotein expressed specifically on the surface of continuous endothelium that binds albumin apparently not only to initiate its transcytosis via plasmalemmal vesicles but also to increase capillary permselectivity.

This paper is referenced by
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E. Durr (2004)
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M. Papisov (1995)
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E. M. Renkin (1994)
Subtractive proteomic mapping of the endothelial surface in lung and solid tumours for tissue-specific therapy
P. Oh (2004)
Albumin in Acute Stroke (ALIAS) Trial-Part 2: A Phase III Randomized Multicenter Clinical Trial of High-Dose Human Albumin Therapy for Neuroprotection in Acute Ischemic Stroke
M. Ginsberg (2010)
Nutritional factors in the prevention and management of coronary artery disease and heart failure.
U. Das (2015)
Nanoparticle Albumin-Bound Anticancer Agents
N. Desai (2015)
Transcytosis: crossing cellular barriers.
P. Tuma (2003)
Characteristics of albumin binding to opossum kidney cells and identification of potential receptors
N. Brunskill (1997)
Vesicle formation and trafficking in endothelial cells and regulation of endothelial barrier function
Richard D. Minshall (2001)
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R. Grebe (2019)
Endothelial Cell-Surface Gp60 Activates Vesicle Formation and Trafficking via Gi-Coupled Src Kinase Signaling Pathway
R. Minshall (2000)
Renal albumin absorption in physiology and pathology.
H. Birn (2006)
Investigational Therapies for Ischemic Stroke: Neuroprotection and Neurorecovery
P. Sahota (2011)
S. Covey (2003)
The protein corona of polymeric nanocarriers: characterization and impact on cellular uptake
S. Schöttler (2015)
The multiple facets of FcRn in immunity
N. M. Stapleton (2015)
Functional and morphological studies of protein transcytosis in continuous endothelia.
D. Predescu (2004)
Endocytosis of nanomedicines.
G. Sahay (2010)
Nanoparticle Albumin-Bound Paclitaxel (Abraxane®)
N. Desai (2016)
Molecular Communication and Nanonetwork for Targeted Drug Delivery: A Survey
U. Chude-Okonkwo (2017)
The blood-brain barrier: Cellular basis
R. Janzer (2004)
Homing of negatively charged albumins to the lymphatic system: general implications for drug targeting to peripheral tissues and viral reservoirs.
P. J. Swart (1999)
Tyrosine phosphorylation of caveolin-1 in the endothelium.
T. Aoki (1999)
Cytoskeletal regulation of pulmonary vascular permeability.
S. Dudek (2001)
Elektronenmikroskopische Analyse des Einflusses von Hydroxyethylstärke auf die endotheliale Permeabilität während des frühen akuten Lungenschadens in vivo
A. Basedow (2010)
Giant membrane vesicles as a model to study cellular substrate uptake dissected from metabolism
D.P.Y. Koonen (2004)
Albumin: A Versatile Drug Carrier
Effect of hyperlipidemic diets, proinflammatory agents and plasma expanders on leukocyte adhesion to the endothelium of aorta and carotid artery of rats.
M. Gabaldón (1994)
Update on the cellular and molecular basis of capillary permeability.
J. Schnitzer (1993)
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