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Pulmonary Vascular Injury By Polycations In Perfused Rat Lungs.

S. Chang, J. Westcott, J. Henson, N. Voelkel
Published 1987 · Medicine

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Polycations, such as protamine sulfate and polylysine, have been implicated in the cause of pulmonary edema, but the mechanism is unknown. We studied the vascular effect of protamine in isolated rat lungs perfused with a cell- and plasma-free solution. Protamine (50-1,000 micrograms/ml) increased lung perfusion pressure and caused edema. Blocking the pulmonary vasoconstriction with papaverine (10(-4) M) did not prevent lung edema. In addition, lungs treated with protamine and papaverine showed increased extravascular leakage of 125I-albumin, indicating increased vascular permeability. Histological examination of these lungs showed marked endothelial injury. Functional endothelial damage was further demonstrated by the impairment of the acetylcholine-induced vascular relaxation in protamine-treated vascular rings. Antihistamines and indomethacin failed to block the pulmonary vasoconstriction and increased vascular permeability caused by protamine. In addition, we found that anionic substances, heparin and albumin, blocked the lung injury induced by protamine, whereas other polycations, polylysine and hexadimethrine bromide, caused pulmonary vasoconstriction and increased vascular permeability similar to protamine. We conclude that protamine causes pulmonary endothelial injury and lung edema and suggest that the injury may be charge mediated.



This paper is referenced by
10.1152/AJPCELL.1995.269.1.C103
Extracellular matrix hyaluronan is a determinant of the endothelial barrier.
R. L. Qiao (1995)
10.1007/BF01721682
The adult respiratory distress syndrome
N. Voelkel (2005)
10.1016/J.BIOMATERIALS.2006.04.029
High efficiency gene transfer using chitosan/DNA nanoparticles with specific combinations of molecular weight and degree of deacetylation.
M. Lavertu (2006)
10.1080/10611860290016766
Biocompatibility of Polycations: In Vitro Agglutination and Lysis of Red Blood Cells And In Vivo Toxicity
E. Moreau (2002)
10.1016/j.biomaterials.2009.07.017
The suppression of lung tumorigenesis by aerosol-delivered folate-chitosan-graft-polyethylenimine/Akt1 shRNA complexes through the Akt signaling pathway.
Hu-Lin Jiang (2009)
10.1111/j.1399-6576.1995.tb04150.x
Effects of heparin on the inhibitory action of protamine on endothelium‐mediated vasorelaxation
T. Akata (1995)
10.1023/A:1016444808000
Chitosans as Absorption Enhancers for Poorly Absorbable Drugs. 1: Influence of Molecular Weight and Degree of Acetylation on Drug Transport Across Human Intestinal Epithelial (Caco-2) Cells
N. G. Schipper (2004)
10.1016/S0741-5214(05)80016-3
Effective and less toxic reversal of low-molecular weight heparin anticoagulation by a designer variant of protamine.
T. Wakefield (1995)
10.1038/sj.gt.3302997
Galactosylated chitosan-graft-polyethylenimine as a gene carrier for hepatocyte targeting
H-L Jiang (2007)
10.1016/0741-5214(92)90416-6
Heparin-mediated reductions of the toxic effects of protamine sulfate on rabbit myocardium.
T. Wakefield (1992)
10.1016/S0378-5173(96)80002-Y
Oral absorption studies of lipid-polylysine conjugates of thyrotropin releasing hormone (TRH1) and luteinizing hormone releasing hormone (LHRH1)
N. Flinn (1996)
10.1164/AJRCCM/147.4.982
The effect of human eosinophil granule major basic protein on airway responsiveness in the rat in vivo. A comparison with polycations.
D. Uchida (1993)
10.11389/JJRS1963.30.1788
[Estimation of pulmonary vascular permeability by indocyanine green in permeability pulmonary edema].
S. Kato (1992)
10.1164/AJRCCM/139.2.534
Charge-related lung microvascular injury.
S. Chang (1989)
10.1016/j.phrs.2015.09.008
Charge is an important determinant of hemodynamic and adverse cardiovascular effects of cationic drugs.
M. Pugsley (2015)
10.1023/B:PHAM.0000016249.52831.a5
Uptake and Cytotoxicity of Chitosan Molecules and Nanoparticles: Effects of Molecular Weight and Degree of Deacetylation
M. Huang (2004)
10.1016/j.carbpol.2014.09.078
Thiolated nanocarriers for oral delivery of hydrophilic macromolecular drugs.
S. Dünnhaupt (2015)
10.1177/026765919701200204
The effect of protamine on the epicardial microflow and the graft flow in open-heart surgery
A. Belboul (1997)
10.1016/S0040-8166(96)80030-9
Distribution of anionic sites on microvascular endothelium of the chick chorioallantoic membrane.
C. B. Henry (1996)
10.3390/ma13215005
Physicochemical Properties and Cell Viability of Shrimp Chitosan Films as Affected by Film Casting Solvents. I-Potential Use as Wound Dressing
H. Y. C. Eulálio (2020)
10.1152/JAPPLPHYSIOL.00175.2002
Rate of NO scavenging alters effects of recombinant hemoglobin solutions on pulmonary vasoreactivity.
T. Resta (2002)
10.1016/S0950-821X(05)80459-1
Heparin and protamine use in peripheral vascular surgery: a comparison between surgeons of the Society for Vascular Surgery and the European Society for Vascular Surgery.
T. Wakefield (1994)
10.1016/S0006-8993(97)01039-1
Effects of the bile salt sodium deoxycholate, protamine, and inflammatory mediators on the potassium permeability of the frog nerve perineurium
B. A. Todd (1997)
10.26182/5ED09E194BB81
Endothelial glycocalyx biomarkers in sepsis and trauma: associations with inflammation, organ failure and bolus fluid therapy.
L. Smart (2020)
10.1016/J.EJPHAR.2007.06.031
Activation of protein kinase B/Akt and endothelial nitric oxide synthase mediates agmatine-induced endothelium-dependent relaxation.
A. Santhanam (2007)
10.1152/AJPCELL.1994.267.4.C1013
Mammalian urinary bladder permeability is altered by cationic proteins: modulation by divalent cations.
C. J. Tzan (1994)
10.1016/0022-4804(91)90023-F
Increased prostacyclin and adverse hemodynamic responses to protamine sulfate in an experimental canine model.
T. Wakefield (1991)
10.1152/JAPPL.1994.77.2.1030
Evans blue dye in the assessment of permeability-surface area product in perfused rat lungs.
M. Dallal (1994)
10.1111/j.1476-5381.1994.tb14019.x
Contractile properties of synthetic cationic polypeptides in guinea‐pig isolated trachea
D. Spina (1994)
10.1247/CSF.29.165
Protamine-induced epithelial barrier disruption involves rearrangement of cytoskeleton and decreased tight junction-associated protein expression in cultured MDCK strains.
E. Peixoto (2005)
10.1097/00004647-199704000-00013
Nitric Oxide and Effects of Cationic Polypeptides in Canine Cerebral Arteries
H. Kinoshita (1997)
10.1164/AJRCCM/147.4.896
Cationic proteins induce airway hyperresponsiveness dependent on charge interactions.
A. Coyle (1993)
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