← Back to Search
Weight Reduction Increases Adipose But Decreases Cardiac LPL In Reduced-obese Zucker Rats.
D. Bessesen, A. D. Robertson, R. Eckel
Published 1991 · Biology, Medicine
Download PDFAnalyze on Scholarcy
Lipoprotein lipase (LPL) activity and mRNA levels were measured in cardiac muscle and adipose tissue from lean, obese, and weight-stable reduced-obese Zucker rats, both fasted and 2 h after feeding. Fasting epididymal fat LPL activity was substantially higher in obese rats relative to lean rats [6.9 vs. 0.2 nmol free fatty acid (FFA).10(6) cells-1.min-1; P = 0.0001], and was higher still in reduced-obese rats (15.7 nmol FFA.10(6) cells-1.min-1; P = 0.002). Adipose tissue LPL increased with feeding in all three groups. In marked contrast, fasting cardiac muscle LPL was lower in obese rats relative to lean (28.8 vs. 38.5 nmol FFA.g-1.min-1; P = 0.0064) and was lower still in reduced-obese rats (14.5 nmol FFA.g-1.min-1; P = 0.0001). LPL mRNA levels increased in adipose tissue along with enzyme activity; however, the magnitude of the changes were relatively small, suggesting that the primary regulatory steps are posttranslational. Weight reduction studies were also carried out in Sprague-Dawley rats with similar results. These studies show that sustained weight reduction results in coordinate changes in tissue-specific LPL, favoring delivery of lipoprotein triglyceride fatty acids to adipose tissue relative to cardiac muscle and the restoration of energy stores.
This paper references
Plasma lipoprotein metabolism in lean and in fat chickens produced by divergent selection for plasma very low density lipoprotein concentration.
H. Griffin (1989)
Increased adipose tissue lipoprotein lipase activity during the development of the genetically obese rat (fa/fa).
R. Gruen (1978)
Lipoprotein lipase gene expression in rat adipocytes is regulated by isoproterenol and insulin through different mechanisms.
M. Raynolds (1990)
Genetic and developmental regulation of the lipoprotein lipase gene: loci both distal and proximal to the lipoprotein lipase structural gene control enzyme expression.
T. Kirchgessner (1989)
Lipoprotein lipase. A multifunctional enzyme relevant to common metabolic diseases.
R. Eckel (1989)
Lipoprotein lipase and hepatic lipase mRNA tissue specific expression, developmental regulation, and evolution.
C. Semenkovich (1989)
The effects of weight loss on the activity and expression of adipose-tissue lipoprotein lipase in very obese humans.
P. Kern (1990)
Localization of lipoprotein lipase mRNA in selected rat tissues.
I. Goldberg (1989)
Increased lipoprotein lipase content in the adipose tissue of suckling and weaning obese Zucker rats.
I. Dugail (1988)
Enzymatic alterations in the obese: long or short-term regulatory errors?
M. R. Greenwood (1984)
Insulin stimulation of adipose tissue lipoprotein lipase. Use of the euglycemic clamp technique.
C. Sadur (1982)
Insulin regulation of lipoprotein lipase activity in 3T3-L1 adipocytes is mediated at posttranscriptional and posttranslational levels.
C. Semenkovich (1989)
The influence of starvation and refeeding on the lipoprotein lipase activity of skeletal muscle and adipose tissue of lean and obese Zucker rats.
D. W. Quig (1983)
Regulation of lipoprotein lipase in different rat tissues.
M. Kuwajima (1988)
Fat calories may be preferentially stored in reduced-obese women: a permissive pathway for resumption of the obese state.
T. J. Yost (1988)
Effect of feeding and obesity on lipoprotein lipase activity, immunoreactive protein, and messenger RNA levels in human adipose tissue.
J. Ong (1989)
Meal-induced changes in lipoprotein lipase activity in brown fat and other tissues of rats.
L. A. Smolin (1986)
Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
P. Chomczyński (1987)
Effects of long-term moderate food restriction on growth, serum factors, lipogenic enzymes and adipocyte glucose metabolism in lean and obese Zucker rats.
M. Cleary (1987)
Lipoprotein lipase activity and cellularity in brown and white adipose tissue in Zucker obese rats.
B. Horwitz (1984)
A simple method to determine fat cell size and number in four mammalian species.
M. di Girolamo (1971)
Tissue-specific regulation of guinea pig lipoprotein lipase; effects of nutritional state and of tumor necrosis factor on mRNA levels in adipose tissue, heart and liver.
S. Enerbäck (1988)
Nutritional regulation of lipoprotein lipase in guinea pig tissues.
H. Semb (1986)
Regulation of lipoprotein lipase activity in the sand rat: effect of nutritional state and cAMP modulation.
T. Chajek-Shaul (1988)
Lipoprotein metabolism influenced by training-induced changes in human skeletal muscle.
B. Kiens (1989)
The response of lipoprotein lipase to feeding and fasting. Evidence for posttranslational regulation.
M. Doolittle (1990)
METABOLISM OF ISOLATED FAT CELLS. I. EFFECTS OF HORMONES ON GLUCOSE METABOLISM AND LIPOLYSIS.
M. Rodbell (1964)
This paper is referenced by
Resistance of adipose tissue lipoprotein lipase to insulin action in rats fed an obesity-promoting diet.
F. Picard (2002)
Increased glyceroneogenesis in adipose tissue from rats adapted to a high-protein, carbohydrate-free diet: role of dietary fatty acids.
S. Brito (2006)
PDFlib PLOP: PDF Linearization, Optimization, Protection Page inserted by evaluation version
S. Utiyama (2001)
Unususal increase of lipogenesis in rat white adipose tissue after multiple cycles of starvation-refeeding.
Z. Kochan (1997)
Chylomicron and palmitate metabolism by perfused hearts from diabetic mice.
A. S. Neitzel (2003)
Dietary D-psicose, a C-3 epimer of D-fructose, suppresses the activity of hepatic lipogenic enzymes in rats.
T. Matsuo (2001)
Lipoprotein lipase: physiology, biochemistry, and molecular biology.
I. Goldberg (2001)
Contribution of hyperinsulinemia to modulation of lipoprotein lipase activity in the obese Zucker rat.
A. Boivin (2000)
Less Body Fat Accumulation with D-Psicose Diet versus D-Fructose Diet
T. Matsuo (2001)
Papel del tejido adiposo blanco en las complicaciones vasculares asociadas a la obesidad
Almudena Gómez-Hernández (2013)
NO-1886 (ibrolipim), a lipoprotein lipase activator, increases the expression of uncoupling protein 3 in skeletal muscle and suppresses fat accumulation in high-fat diet-induced obesity in rats.
M. Kusunoki (2005)
Physiological regulation of lipoprotein lipase.
S. Kersten (2014)
Trafficking of dietary oleic, linolenic, and stearic acids in fasted or fed lean rats.
D. Bessesen (2000)
Intra-Abdominal Fat Adipocyte Hypertrophy through a Progressive Alteration of Lipolysis and Lipogenesis in Metabolic Syndrome Rats
I. Pérez-Torres (2019)
Abnormal A1 adenosine receptor function in genetic obesity
K. Lanoue (1994)
Trafficking of dietary fat in obesity-prone and obesity-resistant rats.
M. Jackman (2006)
Trafficking of dietary fat and resistance to obesity
D. Bessesen (2008)
Overexpression of lipoprotein lipase improves insulin resistance induced by a high-fat diet in transgenic rabbits
S. Kitajima (2004)
Lipoprotein lipase: structure, function, regulation, and role in disease
J. R. Mead (2002)
Global O-GlcNAc Levels Modulate Transcription of the Adipocyte Secretome during Chronic Insulin Resistance
Edith E. Wollaston-Hayden (2015)
Effect of Diet on Respiratory Fuel Mixture, Insulin Sensitivity, and Autonomic Nervous System Function.
H. Roy (1996)
Hepatic and Adipose Tissue Depot-Specific Changes in Lipid Metabolism in Late-Onset Obese (LOB) Rats
F. Frick (2008)
Suppression of carcass weight loss in cachexia in rats bearing Leydig cell tumor by the novel compound NO-1886, a lipoprotein lipase activator.
Masayuki Ohara (1998)
LIPOPROTEIN LIPASE: FROM GENE TO OBESITY Running Title: LPL and Obesity
H. Wang (2009)
Alteration in lipoprotein lipase activity bound to triglyceride-rich lipoproteins in the postprandial state in type 2 diabetes Published, JLR Papers in Press, February 16, 2004. DOI 10.1194/jlr.M300435-JLR200
V. Pruneta-Deloche (2004)
action in rats fed an obesity-promoting diet Resistance of adipose tissue lipoprotein lipase to insulin
Frédéric Picard (2016)
Lipoprotein lipase: from gene to obesity.
H. Wang (2009)
Regulation of adipose tissue lipoprotein lipase in young and old rats
M. Bergö (1997)
The effects of high-fat diet feeding over generations on body fat accumulation associated with lipoprotein lipase and leptin in rat adipose tissues.
Q. Wu (1999)
Skeletal Muscle–Specific Deletion of Lipoprotein Lipase Enhances Insulin Signaling in Skeletal Muscle but Causes Insulin Resistance in Liver and Other Tissues
H. Wang (2008)
Dietary fat is shunted away from oxidation, toward storage in obese Zucker rats.
D. Bessesen (1995)
Peptide Designed to Elicit Apoptosis in Adipose Tissue Endothelium Reduces Food Intake and Body Weight
D. Kim (2010)See more