Online citations, reference lists, and bibliographies.
← Back to Search

Characterization Of PEBBLEs As A Tool For Real-Time Measurement Of Dictyostelium Discoideum Endosomal PH

Everett J. Moding, Jessica Hellyer, Kevin Rank, P. Lostroh, M. Brasuel
Published 2009 · Computer Science, Chemistry

Save to my Library
Download PDF
Analyze on Scholarcy
Share
The measurement of intracellular ion concentration change is important for understanding the cellular mechanisms for communication. Recently developed nanosensors, (Photonic Explorers for Biomedical use with Biologically Localized Embedding) PEBBLEs, have a number of advantages for measuring ions in cells over established methods using microelectrodes, unbound fluorescent dyes, or NMR. PEBBLE sensors have been shown to work in principle for measuring dynamic ion changes, but few in vivo applications have been demonstrated. We modified the protocol for the fabrication of pH sensing PEBBLEs and developed a protocol for the utilization of these sensors for the monitoring of dynamic pH changes in the endosomes of slime mold Dictyostelium discoideum (D. discoideum). Oregon Green 514-CdSe Quantum Dot PEBBLEs were used to measure real-time pH inside D. discoideum endosomes during cAMP stimulation. Endosomal pH was shown to decrease during cAMP signaling, demonstrating a movement of protons into the endosomes of D. discoideum amoebae.
This paper references
10.1083/jcb.200412145
A developmentally regulated Na-H exchanger in Dictyostelium discoideum is necessary for cell polarity during chemotaxis
H. Patel (2005)
10.1073/PNAS.88.11.4951
Regulation of movement speed by intracellular pH during Dictyostelium discoideum chemotaxis.
B. van Duijn (1991)
10.1021/JA072522L
Ion-selective nano-optodes incorporating quantum dots.
J. Dubach (2007)
10.1021/CM052750J
Synthesis and Characterization of Ratiometric, pH Sensing Nanoparticles with Covalently Attached Fluorescent Dyes
H. Sun (2006)
10.1039/B108568A
A fluorescent PEBBLE nanosensor for intracellular free zinc.
J. P. Sumner (2002)
10.1021/AC990629O
Optical nanosensors for chemical analysis inside single living cells. 1. Fabrication, characterization, and methods for intracellular delivery of PEBBLE sensors.
H. Clark (1999)
10.1074/JBC.R300010200
Eukaryotic Chemotaxis: Distinctions between Directional Sensing and Polarization*
P. Devreotes (2003)
10.1007/BF02703931
Mechanism of cAMP-induced H+-efflux ofDictyostelium cells: a role for fatty acids
H. Flaadt (2007)
10.1111/J.1399-3054.1995.TB00846.X
FITC‐dextran for measuring apoplast pH and apoplastic pH gradients between various cell types in sunflower leaves
B. Hoffmann (1995)
10.1021/AC0342323
Ratiometric optical PEBBLE nanosensors for real-time magnesium ion concentrations inside viable cells.
E. Park (2003)
Tested studies for laboratory teaching
D. M. Bozzone (1993)
10.1128/JB.149.1.99-105.1982
Analogs of cyclic AMP as chemoattractants and inhibitors of Dictyostelium chemotaxis.
P. J. V. Van Haastert (1982)
Measurement of cytoplasmic pH in Dictyostelium discoideum by using a new method for introducing macromolecules into living cells.
M. Fechheimer (1986)
10.1002/JEMT.20282
Spectral characterization of Dictyostelium autofluorescence
R. Engel (2006)
10.1016/0300-9084(92)90072-M
Kinetics of endosomal acidification in Dictyostelium discoideum amoebae. 31P-NMR evidence for a very acidic early endosomal compartment.
F. Brénot (1992)
Measurements of intracellular pH and its relevance to cell differentiation in Dictyostelium discoideum.
K. Inouye (1985)
10.1021/AC990630N
Optical nanosensors for chemical analysis inside single living cells. 2. Sensors for pH and calcium and the intracellular application of PEBBLE sensors.
H. Clark (1999)
10.1016/0012-1606(87)90300-9
Relationships between extracellular pH, intracellular pH, and gene expression in Dictyostelium discoideum.
C. Town (1987)
10.1083/JCB.101.3.778
Intracellular pH in Dictyostelium discoideum: a 31P nuclear magnetic resonance study
J. Jentoft (1985)
10.1083/JCB.99.5.1883
Transient increase in intracellular pH during Dictyostelium differentiation
G. Jamieson (1984)
10.1016/S0960-894X(99)00512-0
Synthesis and fluorescence properties of Oregon Green 514 labeled peptides.
C. Delmotte (1999)
10.1021/AC0701233
Multicolor quantum dot encoding for polymeric particle-based optical ion sensors.
C. Xu (2007)
10.1007/S00216-007-1244-9
Noninvasive monitoring of intracellular pH change induced by drug stimulation using silica nanoparticle sensors
Jiaofeng Peng (2007)
10.1016/0014-5793(87)80848-7
Cyclic AMP induces a transient alkalinization in Dictyostelium
R. J. Aerts (1987)
10.1515/BCHM2.1984.365.2.1255
Extracellular lumazine from aggregating Dictyostelium discoideum cells. Influence of pH on its fluorescence.
I. Tatischeff (1984)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar