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Preparation And Characterization Of Self-Assembled Nanoparticles Of Hyaluronic Acid-Deoxycholic Acid Conjugates

Xuemeng Dong, Chenguang Liu

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Novel amphiphilic biopolymers were synthesized using hyaluronic acid (HA) as a hydrophilic segment and deoxycholic acid (DOCA) as a hydrophobic segment by a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide mediated coupling reaction. The structural characteristics of the HA-DOCA conjugates were investigated usingH1NMR. Self-assembled nanoparticles were prepared based on HA-DOCA conjugates, and its characteristics were investigated using dynamic laser light scattering, transmission electron microscopy (TEM), and fluorescence spectroscopy. The mean diameter was about 293.5 nm with unimodal size distribution in distilled water. The TEM images revealed that the shape of HA-DOCA self-aggregates was spherical. The critical aggregation concentration (CAC) was in the range of 0.025–0.056 mg/mL. The partition equilibrium constant (Kv) of pyrene in self-aggregates solution was from1.45×104to3.64×104. The aggregation number of DOCA groups per hydrophobic microdomain, estimated by the fluorescence quenching method using cetylpyridinium chloride, increased with increasing degree of substitution.