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GHK And DNA: Resetting The Human Genome To Health

L. Pickart, Jessica Michelle Vasquez-Soltero, Anna Margolina
Published 2014 · Biology, Medicine

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During human aging there is an increase in the activity of inflammatory, cancer promoting, and tissue destructive genes plus a decrease in the activity of regenerative and reparative genes. The human blood tripeptide GHK possesses many positive effects but declines with age. It improves wound healing and tissue regeneration (skin, hair follicles, stomach and intestinal linings, and boney tissue), increases collagen and glycosaminoglycans, stimulates synthesis of decorin, increases angiogenesis, and nerve outgrowth, possesses antioxidant and anti-inflammatory effects, and increases cellular stemness and the secretion of trophic factors by mesenchymal stem cells. Recently, GHK has been found to reset genes of diseased cells from patients with cancer or COPD to a more healthy state. Cancer cells reset their programmed cell death system while COPD patients' cells shut down tissue destructive genes and stimulate repair and remodeling activities. In this paper, we discuss GHK's effect on genes that suppress fibrinogen synthesis, the insulin/insulin-like system, and cancer growth plus activation of genes that increase the ubiquitin-proteasome system, DNA repair, antioxidant systems, and healing by the TGF beta superfamily. A variety of methods and dosages to effectively use GHK to reset genes to a healthier state are also discussed.
This paper references
10.3109/01902148609061488
The effect of culture conditions on cytodifferentiation of fetal mouse lung respiratory passageways.
S. R. Hilfer (1986)
10.1097/00019616-199611000-00017
Fibrinogen Is a Predictor of Mortality in Coronary Heart Disease Patients
MichalBenderly (1996)
10.1111/j.1467-2494.2005.00279.x
New anti‐RNS and ‐RCS products for cosmetic treatment
J. Cebrian (2005)
10.1111/j.1469-1809.2009.00551.x
Interaction between Fibrinogen and IL‐6 Genetic Variants and Associations with Cardiovascular Disease Risk in the Cardiovascular Health Study
C. Carty (2010)
10.1038/ejhg.2013.299
Human longevity and variation in DNA damage response and repair: study of the contribution of sub-processes using competitive gene-set analysis
B. Debrabant (2014)
10.1002/jcb.22617
Ferroportin1 and hephaestin overexpression attenuate iron‐induced oxidative stress in MES23.5 dopaminergic cells
Ning Song (2010)
10.1186/1752-0509-8-27
Stress induced telomere shortening: longer life with less mutations?
A. Trusina (2012)
10.1016/S1074-7613(00)80013-8
Interleukin-18 binding protein: a novel modulator of the Th1 cytokine response.
D. Novick (1999)
Lipid regulation in SZ95 sebocytes by glycyl-histidyl-lysine
S. Schagen (2007)
Method of using copper(II) containing compounds to accelerate wound healing
L. Pickart (1992)
10.1007/978-1-4684-5730-8_11
Effects of glycyl-histidyl-lysyl chelated Cu(II) on ferritin dependent lipid peroxidation.
D. M. Miller (1990)
10.1038/ni.2391
Restraint of inflammatory signaling by interdependent strata of negative regulatory pathways
P. Murray (2012)
10.1002/JBM.B.30246
Biotinylated GHK peptide incorporated collagenous matrix: A novel biomaterial for dermal wound healing in rats.
V. Arul (2005)
10.1038/354341c0
Nature Genetics
(1991)
10.1016/0197-0186(95)00114-X
ROLE OF METALLOTHIONEIN AND OTHER ANTIOXIDANTS IN SCAVENGING SUPEROXIDE RADICALS AND THEIR POSSIBLE ROLE IN NEUROPROTECTION
S. Hussain (1996)
10.1161/01.ATV.21.6.1065
Plasma Fibrinogen as a Predictor of Total and Cause-Specific Mortality in Elderly Japanese-American Men
K. Yano (2001)
Effect of Gly-Gly-His, Gly-His-Lys and their copper complexes on TNF-alpha-dependent IL-6 secretion in normal human dermal fibroblasts.
A. Gruchlik (2012)
10.1016/0005-2760(92)90256-U
The influence of medium components on Cu(2+)-dependent oxidation of low-density lipoproteins and its sensitivity to superoxide dismutase.
C. Thomas (1992)
10.1517/17425247.2012.717068
Oral delivery of peptides and proteins using lipid-based drug delivery systems
P. Li (2012)
10.1186/1471-230X-14-44
Hepatoprotective role of liver fatty acid binding protein in acetaminophen induced toxicity
Y. Gong (2014)
BioMed Research International
Method of using copper(II) containing compounds to accelerate wound healing United States Patent 5164367
L Pickart (1992)
10.1159/000353857
Aging-Related Genes in Mesenchymal Stem Cells: A Mini-Review
Kyung-Rok Yu (2013)
10.1093/gerona/glu042
Epigenetics of aging and aging-related disease.
A. Brunet (2014)
10.1152/AJPHEART.00376.2007
TNF-alpha-induced mitochondrial oxidative stress and cardiac dysfunction: restoration by superoxide dismutase mimetic Tempol.
N. Mariappan (2007)
10.1021/pr300310b
Proteomic identification of in vivo interactors reveals novel function of skin cornification proteins.
W. P. Vermeij (2012)
Antioxi - dant role ofmetallothioneins : a comparative overview
R. A. Momin (2000)
10.1021/TX700185S
Glycyl-histidyl-lysine (GHK) is a quencher of alpha,beta-4-hydroxy-trans-2-nonenal: a comparison with carnosine. insights into the mechanism of reaction by electrospray ionization mass spectrometry, 1H NMR, and computational techniques.
G. Beretta (2007)
10.1002/psc.2455
Stem cell recovering effect of copper‐free GHK in skin
H. Choi (2012)
10.1126/science.1132939
The Connectivity Map: Using Gene-Expression Signatures to Connect Small Molecules, Genes, and Disease
J. Lamb (2006)
10.1016/j.jpba.2008.02.012
Acrolein sequestering ability of the endogenous tripeptide glycyl-histidyl-lysine (GHK): characterization of conjugation products by ESI-MSn and theoretical calculations.
G. Beretta (2008)
10.1098/rstb.2010.0276
The first long-lived mutants: discovery of the insulin/IGF-1 pathway for ageing
C. Kenyon (2011)
10.4172/2155-952X.1000144
The Tripeptide, GHK, Induces Programmed Cell Death in SH-SY5Y Neuroblastoma Cells
Luay E. Matalka (2012)
NF-í µí¼…B-induced oxidative stress contributes to mitochondrial and cardiac dysfunction in type II diabetes
N Mariappan (2010)
10.1152/ajpendo.00357.2011
Disruption of paraoxonase 3 impairs proliferation and antioxidant defenses in human A549 cells and causes embryonic lethality in mice.
Sarah L. Kempster (2012)
10.1042/BJ2630539
Human fibroblasts release reactive oxygen species in response to interleukin-1 or tumour necrosis factor-alpha.
B. Meier (1989)
10.1007/s10585-010-9305-4
A ‘metastasis-prone’ signature for early-stage mismatch-repair proficient sporadic colorectal cancer patients and its implications for possible therapeutics
Y. Hong (2010)
10.1038/nrm3758
Molecular mechanisms of epithelial–mesenchymal transition
Samy Lamouille (2014)
10.1136/jcp.54.3.176
Antioxidants in health and disease
I. Young (2001)
10.1038/ng0506-500
GenePattern 2.0
M. Reich (2006)
10.1007/BF02369351
Copper addition prevents the inhibitory effects of interleukin 1-β on rat pancreatic islets
C. Vinci (2006)
The human tripeptide GHK (Glycyl-L-Histidyl- L-Lysine), " in The Copper Switch, and The Treatment of the Degenerative Conditions of Aging
L Pickart (2009)
10.1089/pho.2012.3312
Gene expression and release of growth factors during delayed wound healing: a review of studies in diabetic animals and possible combined laser phototherapy and growth factor treatment to enhance healing.
P. Peplow (2012)
10.6000/1927-7229.2014.03.02.2
GHK, the Human Skin Remodeling Peptide, Induces Anti-Cancer Expression of Numerous Caspase, Growth Regulatory, and DNA Repair Genes
L. Pickart (2014)
10.1038/nature08980
The genetics of ageing
C. Kenyon (2010)
10.1021/JF0114381
Antioxidant and cyclooxygenase activities of fatty acids found in food.
G. E. Henry (2002)
Synergetic antioxidant and reparative action of thymogen, dalargin and peptide Gly-His-Lys in tubular bone fractures
V. Y. Cherdakov (2010)
10.3181/00379727-4-15
On the fractionation of agglutinins and antitoxin
R. Gibson (1906)
10.1186/gm367
A gene expression signature of emphysema-related lung destruction and its reversal by the tripeptide GHK
J. Campbell (2012)
10.1007/s11010-010-0457-y
Wound repair and anti-oxidative capacity is regulated by ITGB4 in airway epithelial cells
Chi Liu (2010)
10.1038/nsmb.2806
Ubiquitin sets the timer: impacts on aging and longevity
Éva Kevei (2014)
10.1016/J.ATHEROSCLEROSIS.2005.08.026
Paraoxonase 1 (PON1) is a more potent antioxidant and stimulant of macrophage cholesterol efflux, when present in HDL than in lipoprotein-deficient serum: relevance to diabetes.
M. Rosenblat (2006)
Mollecular Biology of the Cell
H Lodish (2004)
10.1093/gerona/glu057
Chronic inflammation (inflammaging) and its potential contribution to age-associated diseases.
C. Franceschi (2014)
10.2174/1381612043452901
Induction of cytoprotective genes through Nrf2/antioxidant response element pathway: a new therapeutic approach for the treatment of inflammatory diseases.
X. Chen (2004)
10.1016/j.mayocp.2014.01.020
Targets of immune regeneration in rheumatoid arthritis.
P. Hohensinner (2014)
10.4161/cam.22164
The dual roles of homeobox genes in vascularization and wound healing
Suraj Kachgal (2012)
10.1016/J.LFS.2006.09.018
A therapeutic approach for diabetic wound healing using biotinylated GHK incorporated collagen matrices.
V. Arul (2007)
10.1016/j.atherosclerosis.2011.11.031
Apolipoprotein M binds oxidized phospholipids and increases the antioxidant effect of HDL.
Sara Elsøe (2012)
Antioxi - dant role ofmetallothioneins : a comparative overview
G. E. Henry (2000)
10.1186/1479-5876-5-11
Gene expression profiling of cutaneous wound healing
Kavita Deonarine (2006)
10.1089/WOUND.2012.0386
A Network Approach to Wound Healing.
Tom Arodz (2013)
NF-B-induced oxidative stress contributes to mitochondrial and cardiac dysfunction in type II diabetes
N Mariappan (2010)
10.1007/s00011-010-0238-9
Human skin penetration of a copper tripeptide in vitro as a function of skin layer
Jurij J. Hostynek (2011)
10.1016/j.actbio.2014.01.020
Enhanced trophic factor secretion by mesenchymal stem/stromal cells with Glycine-Histidine-Lysine (GHK)-modified alginate hydrogels.
Soumia Jose (2014)
10.1038/jid.2010.433
ROS quenching potential of the epidermal cornified cell envelope.
W. P. Vermeij (2011)
10.1517/17425247.2012.735658
Liposomal delivery of proteins and peptides
Janani Swaminathan (2012)
NF-κB-induced oxidative stress contributes to mitochondrial and cardiac dysfunction in type II diabetes
N. Mariappan (2010)
The human tripeptide GHK (Glycyl-L-Histidyl- L-Lysine)
L. Pickart (2009)
Reparative activity of different functional group peptides in hepatopathyes
M. Y. Smakhtin (2006)
Hepatoprotective role of liver fatty acid binding protein in BioMed Research International 9 acetaminophen induced toxicity
Y. Gong (2014)
The influence of medium components on Cu-dependent oxidation of low-density lipoproteins and its sensitivity to superoxide dismutase
C. E. Thomas (1992)
10.1016/j.freeradbiomed.2014.03.031
Proteasome activation delays aging in vitro and in vivo.
N. Chondrogianni (2014)
10.1053/JOCA.2000.0400
Sodium hyaluronate inhibits interleukin-1-evoked reactive oxygen species of bovine articular chondrocytes.
K. Fukuda (2001)
10.1016/j.neuint.2012.02.009
Protein homeostasis and aging: Role of ubiquitin protein ligases
N. Jana (2012)
Antioxidant role of metallothioneins: a comparative overview.
A. Viarengo (2000)
10.1016/0162-0134(92)84013-D
Cytoprotective effect of copper(II) complexes against ethanol-induced damage to rat gastric mucosa.
M. Alberghina (1992)
10.1002/hep.20857
Antioxidative function of L‐FABP in L‐FABP stably transfected Chang liver cells
G. Wang (2005)
10.1163/156856208784909435
The human tri-peptide GHK and tissue remodeling
L. Pickart (2008)



This paper is referenced by
TriHex TechnologyTM from Alastin Skincare powers effective skincare
A. Widgerow (2016)
10.3390/COSMETICS2030236
GHK-Cu may Prevent Oxidative Stress in Skin by Regulating Copper and Modifying Expression of Numerous Antioxidant Genes
L. Pickart (2015)
10.3389/fnins.2018.00644
Glycine-Histidine-Lysine (GHK) Alleviates Neuronal Apoptosis Due to Intracerebral Hemorrhage via the miR-339-5p/VEGFA Pathway
Heyu Zhang (2018)
10.1007/978-3-642-27814-3_162-1
Resetting Skin Genome Back to Health Naturally with GHK
L. Pickart (2015)
10.3390/ijms19071987
Regenerative and Protective Actions of the GHK-Cu Peptide in the Light of the New Gene Data
L. Pickart (2018)
10.1111/wrr.12520
GHK‐Cu‐liposomes accelerate scald wound healing in mice by promoting cell proliferation and angiogenesis
X. Wang (2017)
10.3390/ijms21176190
Ternary Cu(II) Complex with GHK Peptide and Cis-Urocanic Acid as a Potential Physiologically Functional Copper Chelate
Karolina Bossak-Ahmad (2020)
10.1016/j.lfs.2019.117139
Protective effects of GHK-Cu in bleomycin-induced pulmonary fibrosis via anti-oxidative stress and anti-inflammation pathways.
Wen-hui Ma (2019)
10.1016/j.biochi.2015.09.016
Proteases decode the extracellular matrix cryptome.
S. Ricard-Blum (2016)
10.21926/obm.geriatr.1803009
The Effect of the Human Plasma Molecule GHK-Cu on Stem Cell Actions and Expression of Relevant Genes
L. Pickart (2018)
10.3389/fnins.2020.576389
Glycine-Histidine-Lysine (GHK) Alleviates Astrocytes Injury of Intracerebral Hemorrhage via the Akt/miR-146a-3p/AQP4 Pathway
Heyu Zhang (2020)
Tripeptides, compositions les contenant et leurs utilisations cosmétiques
Olivier Peschard (2015)
10.1007/978-3-030-19962-3_12
Drug Delivery Advances for the Regeneration of Aged Skin
Daniela Castillo Pérez (2019)
10.3390/COSMETICS5020029
Skin Regenerative and Anti-Cancer Actions of Copper Peptides
L. Pickart (2018)
10.1111/jocd.13270
Effects of anhydrous gel with TriHex peptides on healing after hybrid laser resurfacing
A. M. Nelson (2020)
Tripeptides, compositions comprising the same, and uses in particular cosmetics
Olivier Peschard (2014)
10.3390/biomedicines8020038
Long-Term Oral Administration of LLHK, LHK, and HK Alters Gene Expression Profile and Restores Age-Dependent Atrophy and Dysfunction of Rat Salivary Glands
Y. Ishikawa (2020)
A Tripeptide/Hexapeptide Anti-Aging Regimen that Targets Both Collagen and Elastin, and Improves both Physician and Subject Scoring of Facial Aesthetics
Nelson Aislyn (2018)
10.1039/c8mt00221e
Metal-dependent hormone function: the emerging interdisciplinary field of metalloendocrinology.
M. J. Stevenson (2019)
10.3389/fphar.2017.00904
GHK Peptide Inhibits Bleomycin-Induced Pulmonary Fibrosis in Mice by Suppressing TGFβ1/Smad-Mediated Epithelial-to-Mesenchymal Transition
Xiao-ming Zhou (2017)
10.1155/2015/648108
GHK Peptide as a Natural Modulator of Multiple Cellular Pathways in Skin Regeneration
L. Pickart (2015)
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