Online citations, reference lists, and bibliographies.
← Back to Search

Phenotypic Characterization Of Transgenic Miscanthus Sinensis Plants Overexpressing Arabidopsis Phytochrome B

Ok-Jin Hwang, S. Lim, Yun-jeong Han, Ah-Young Shin, Do-Soon Kim, Jeong-Il Kim
Published 2014 · Biology

Cite This
Download PDF
Analyze on Scholarcy
Share
Phytochromes are dimeric pigment proteins with reversible photochromism between red and far-red light-absorbing forms. They are photoreceptors that regulate various aspects of plant growth and development and have been used for biotechnological applications to improve agricultural performance of crops. Miscanthus species have been suggested as one of the most promising energy crops. In this paper, Arabidopsis phytochrome B (PHYB) gene was introduced into Miscanthus sinensis using Agrobacterium-mediated transformation method that we developed recently, with the herbicide resistance gene (BAR) as a selection marker. After putative transgenic plants were selected using the herbicide resistance assay, genomic integration of the transgene was confirmed by genomic PCR and Southern blot analysis, and transgene expression was validated by Northern blot analysis. Compared to nontransformed control plants, transgenic plants overexpressing PHYB showed phenotypes with increased phytochrome B function, which includes increased chlorophyll content, decreased plant height, and delayed flowering. Therefore, these results suggest that Arabidopsis phytochrome B is functional in M. sinensis and provide a method to develop Miscanthus varieties with enhanced agricultural performance using phytochromes.
This paper references
10.1007/978-1-4939-2453-0_7
Transgene-induced gene silencing in plants.
Y. Jin (2015)
10.1038/NBT0896-995
Genetic engineering of harvest index in tobacco through overexpression of a phytochrome gene
Paul R.H. Robson (1996)
10.1016/J.INDCROP.2004.01.004
Establishing Miscanthus sinensis from seed using conventional sowing methods
D. Christian (2005)
10.1023/A:1006464304199
Plants as bioreactors for protein production: avoiding the problem of transgene silencing
C. De Wilde (2004)
10.1126/SCIENCE.1114736
The Path Forward for Biofuels and Biomaterials
A. Ragauskas (2006)
10.1093/pcp/pcq025
Functional characterization of phytochrome autophosphorylation in plant light signaling.
Yun-jeong Han (2010)
10.1199/tab.0148
Phytochrome Signaling Mechanisms
J. Li (2011)
Shade avoidance,”New Phytologist
K. A. Franklin (2008)
10.1105/tpc.5.2.147
Mutations in the gene for the red/far-red light receptor phytochrome B alter cell elongation and physiological responses throughout Arabidopsis development.
J. Reed (1993)
10.1105/tpc.104.023879
Phytochrome Phosphorylation Modulates Light Signaling by Influencing the Protein–Protein Interaction w⃞
Jeong-Il Kim (2004)
10.1105/tpc.3.12.1275
Overexpression of Phytochrome B Induces a Short Hypocotyl Phenotype in Transgenic Arabidopsis.
D. Wagner (1991)
10.1105/tpc.109.072280
Evolutionary Studies Illuminate the Structural-Functional Model of Plant Phytochromes[W]
S. Mathews (2010)
10.1101/GAD.14.3.257
Light: an indicator of time and place.
M. Neff (2000)
10.1631/jzus.B1000168
Overexpression of the phytochrome B gene from Arabidopsis thaliana increases plant growth and yield of cotton (Gossypium hirsutum)
A. Q. Rao (2011)
10.1007/s00299-012-1280-6
Overexpression of an Arabidopsis β-glucosidase gene enhances drought resistance with dwarf phenotype in creeping bentgrass
Yun-jeong Han (2012)
10.1146/ANNUREV.GENET.38.072902.092259
Light signal transduction in higher plants.
Meng Chen (2004)
10.1146/ANNUREV.ARPLANT.56.032604.144208
Phytochrome structure and signaling mechanisms.
N. Rockwell (2006)
10.1073/PNAS.88.23.10806
Phytochrome a overexpression inhibits hypocotyl elongation in transgenic Arabidopsis.
M. Boylan (1991)
Enhanced recovery of transformants of Agrobacterium tumefaciens after freeze-thaw transformation and drug selection.
H. Chen (1994)
10.1111/j.1757-1707.2011.01090.x
Establishment of an efficient in vitro culture and particle bombardment‐mediated transformation systems in Miscanthus sinensis Anderss., a potential bioenergy crop
X. Wang (2011)
10.1104/PP.120.1.73
Heterologous expression of Arabidopsis phytochrome B in transgenic potato influences photosynthetic performance and tuber development.
A. Thiele (1999)
10.3389/fpls.2013.00107
The potential of C4 grasses for cellulosic biofuel production
Tim van der Weijde (2013)
10.1046/J.1365-313X.1997.12051079.X
Expression of heterologous phytochromes A, B or C in transgenic tobacco plants alters vegetative development and flowering time.
K. Halliday (1997)
10.1016/S0961-9534(03)00030-8
The development and current status of perennial rhizomatous grasses as energy crops in the US and Europe
I. Lewandowski (2003)
10.1007/s00299-008-0648-0
Production of purple-colored creeping bentgrass using maize transcription factor genes Pl and Lc through Agrobacterium-mediated transformation
Yun-jeong Han (2008)
10.1051/agro/2009034
Agronomic and physiological performances of different species of Miscanthus, a major energy crop. A review
H. Zub (2012)
10.1046/J.1365-3040.1997.D01-106.X
Fundamental and biotechnological applications of phytochrome transgenes
P. Robson (1997)
10.1093/JXB/ERM205
The molecular analysis of the shade avoidance syndrome in the grasses has begun.
Tesfamichael H Kebrom (2007)
10.1007/s11627-009-9214-x
Genetic improvement of C4 grasses as cellulosic biofuel feedstocks
Katrin Jakob (2009)
10.1146/annurev.arplant.59.032607.092859
Decoding of light signals by plant phytochromes and their interacting proteins.
Gabyong Bae (2008)
10.1007/BF03030650
Phytochrome-mediated photomorphogenesis in plants
Yun-jeong Han (2009)
Establishing from seed using conventional sowing methods
D. Christian (2005)
10.1016/B978-0-12-381518-7.00003-0
Miscanthus: A Promising Biomass Crop
E. Heaton (2010)
10.1111/J.1365-2486.2008.01662.X
Meeting US biofuel goals with less land: the potential of Miscanthus
E. Heaton (2008)
10.1016/S0012-1606(03)00212-4
From seed to seed: the role of photoreceptors in Arabidopsis development.
J. A. Sullivan (2003)
10.1042/BST024517SC
Phytochromes: photosensory perception and signal transduction.
P. Quail (1995)
10.1073/PNAS.92.19.8596
Mutational analysis of phytochrome B identifies a small COOH-terminal-domain region critical for regulatory activity.
D. Wagner (1995)
10.1007/s00438-011-0621-4
Phytochrome B regulates Heading date 1 (Hd1)-mediated expression of rice florigen Hd3a and critical day length in rice
R. Ishikawa (2011)
10.1111/j.1365-313X.2009.04105.x
Light signal transduction: an infinite spectrum of possibilities.
J. Chory (2010)
10.1105/tpc.9.12.2271
Biochemical characterization of Arabidopsis wild-type and mutant phytochrome B holoproteins.
T. Elich (1997)
10.1007/s11240-013-0419-7
Agrobacterium-mediated genetic transformation of Miscanthus sinensis
Ok-Jin Hwang (2013)
10.1038/nrm728
Phytochrome photosensory signalling networks
P. Quail (2002)



This paper is referenced by
PART OF A SPECIAL ISSUE ON BIOBASED VALUE CHAINS FOR A GROWING BIOECONOMY
J. Clifton-Brown (2018)
10.1007/s10118-020-2424-8
Phase Separation in PCDTBT:PCBM Blends: from Flory-Huggins Interaction Parameters to Ternary Phase Diagrams
M. Biernat (2020)
10.1134/s0965545x20030177
A Self-Polishing Polyacrylate- g -polysiloxane Paint for Marine Antifouling Application
Z. Xia (2020)
10.1016/S2095-3119(16)61434-X
The molecular mechanism of shade avoidance in crops- How data from Arabidopsis can help to identify targets for increasing yield and biomass production
Yun-jia Tang (2017)
10.1016/j.biotechadv.2014.12.005
Photo-biotechnology as a tool to improve agronomic traits in crops.
M. Gururani (2015)
10.1007/s40243-018-0132-x
Co-sensitization aided efficiency enhancement in betanin–chlorophyll solar cell
S. Sreeja (2018)
10.1039/c9nj03774h
Lithium ferrite (α-LiFe5O8) nanorod based battery-type asymmetric supercapacitor with NiO nanoflakes as the counter electrode
J. William (2019)
10.1093/jxb/ery258
Exploiting SPL genes to improve maize plant architecture tailored for high-density planting
Hongbin Wei (2018)
10.1007/s11240-016-1078-2
Plant regeneration from calli in Japanese accessions of Miscanthus
W. Takahashi (2016)
10.1016/J.JIEC.2017.09.033
Review on earth-abundant and environmentally benign Cu-Sn-X(X = S, Se) nanoparticles by chemical synthesis for sustainable solar energy conversion
Babu Pejjai (2017)
10.1016/J.IJHYDENE.2016.11.084
Status review on earth-abundant and environmentally green Sn-X (X = Se, S) nanoparticle synthesis by solution methods for photovoltaic applications
Babu Pejjai (2017)
10.1002/pld3.210
Regulation of monocot and dicot plant development with constitutively active alleles of phytochrome B
Wei Hu (2020)
10.1039/C6TC02330D
Fluorescent graphene-like carbon nitrides: synthesis, properties and applications
A. Wang (2016)
10.1039/C5RA10639G
Bioframe synthesis of NF–TiO2/straw charcoal composites for enhanced adsorption-visible light photocatalytic degradation of RhB
X. Wang (2015)
10.1371/journal.pone.0170578
Functional Characterization of Cotton GaMYB62L, a Novel R2R3 TF in Transgenic Arabidopsis
H. I. Butt (2017)
10.1111/gcbb.12566
Breeding progress and preparedness for mass‐scale deployment of perennial lignocellulosic biomass crops switchgrass, miscanthus, willow and poplar
J. Clifton-Brown (2019)
Semantic Scholar Logo Some data provided by SemanticScholar