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Anticancer Potential Of Green Synthesized Silver Nanoparticles Using Extract Of Nepeta Deflersiana Against Human Cervical Cancer Cells (HeLA)

E. Al-sheddi, N. N. Farshori, M. Al-Oqail, S. Al-Massarani, Q. Saquib, R. Wahab, J. Musarrat, A. Al-Khedhairy, M. A. Siddiqui
Published 2018 · Chemistry, Medicine

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In this study, silver nanoparticles (AgNPs) were synthesized using aqueous extract of Nepeta deflersiana plant. The prepared AgNPs (ND-AgNPs) were examined by ultraviolet-visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscope (SEM), and energy dispersive spectroscopy (EDX). The results obtained from various characterizations revealed that average size of synthesized AgNPs was 33 nm and in face-centered-cubic structure. The anticancer potential of ND-AgNPs was investigated against human cervical cancer cells (HeLa). The cytotoxic response was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), neutral red uptake (NRU) assays, and morphological changes. Further, the influence of cytotoxic concentrations of ND-AgNPs on oxidative stress markers, reactive oxygen species (ROS) generation, mitochondrial membrane potential (MMP), cell cycle arrest and apoptosis/necrosis was studied. The cytotoxic response observed was in a concentration-dependent manner. Furthermore, the results also showed a significant increase in ROS and lipid peroxidation (LPO), along with a decrease in MMP and glutathione (GSH) levels. The cell cycle analysis and apoptosis/necrosis assay data exhibited ND-AgNPs-induced SubG1 arrest and apoptotic/necrotic cell death. The biosynthesized AgNPs-induced cell death in HeLA cells suggested the anticancer potential of ND-AgNPs. Therefore, they may be used to treat the cervical cancer cells.
This paper references
10.1002/cmmi.282
Assessing cytotoxicity of (iron oxide-based) nanoparticles: an overview of different methods exemplified with cationic magnetoliposomes.
S. Soenen (2009)
10.1016/J.INDCROP.2013.10.050
Green synthesis and characterization of silver nanoparticles using Boerhaavia diffusa plant extract and their anti bacterial activity
P. P. N. Vijay Kumar (2014)
10.1016/j.colsurfb.2010.02.007
Biosynthesis of silver and gold nanoparticles using Brevibacterium casei.
K. Kalishwaralal (2010)
10.1021/LA010414L
Formation of Colloidal Gold Nanoparticles in an Ultrasonic Field: Control of Rate of Gold(III) Reduction and Size of Formed Gold Particles
K. Okitsu (2001)
10.1021/LA052055Q
Role of halide ions and temperature on the morphology of biologically synthesized gold nanotriangles.
Akhilesh Rai (2006)
10.1002/SMLL.200700378
Size-dependent cytotoxicity of gold nanoparticles.
Y. Pan (2007)
10.1016/j.saa.2012.03.002
Optimization for rapid synthesis of silver nanoparticles and its effect on phytopathogenic fungi.
C. Krishnaraj (2012)
10.1016/j.ijpharm.2011.08.020
Comparative sensitivity of tumor and non-tumor cell lines as a reliable approach for in vitro cytotoxicity screening of lysine-based surfactants with potential pharmaceutical applications.
D. Nogueira (2011)
10.4014/JMB.1310.10095
Fabrication of biogenic antimicrobial silver nanoparticles by Streptomyces aegyptia NEAE 102 as eco-friendly nanofactory.
N. El-Naggar (2014)
10.1016/J.MATLET.2012.02.024
Incorporation of silver nanoparticles on textile materials by an aqueous procedure
I. Osório (2012)
10.1371/journal.pone.0069534
Copper Oxide Nanoparticles Induced Mitochondria Mediated Apoptosis in Human Hepatocarcinoma Cells
M. A. Siddiqui (2013)
10.1016/j.colsurfb.2013.01.037
Biosynthesis, characterization and cytotoxic effect of plant mediated silver nanoparticles using Morinda citrifolia root extract.
T. Y. Suman (2013)
10.1016/j.colsurfb.2011.11.001
Synthesis of silver nanoparticles using Piper longum leaf extracts and its cytotoxic activity against Hep-2 cell line.
S. Jacob (2012)
10.1016/S0927-7757(97)00087-3
pH Dependent changes in the optical properties of carboxylic acid derivatized silver colloidal particles
M. Sastry (1997)
10.1016/j.mrgentox.2012.01.001
Cytotoxic and necrotic responses in human amniotic epithelial (WISH) cells exposed to organophosphate insecticide phorate.
Q. Saquib (2012)
Evaluation of the in vitro anticancer, antimicrobial and antioxidant activities of some Yemeni plants used in folk medicine.
R. A. Mothana (2009)
10.3945/an.116.012211
World Cancer Report 2014. Geneva, Switzerland: World Health Organization, International Agency for Research on Cancer, WHO Press, 2015.
Shelley McGuire (2016)
10.2147/IJN.S71182
Potential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles
K. D. Arunachalam (2015)
10.1016/j.nano.2011.06.014
Antibacterial activity, inflammatory response, coagulation and cytotoxicity effects of silver nanoparticles.
F. Martinez-Gutierrez (2012)
thesis , characterization , and evaluation of antimicrobial and cytotoxic effect of silver and titanium nanoparticle
P. Corbeau
10.1038/NMAT758
Biomimetic synthesis and patterning of silver nanoparticles
R. Naik (2002)
10.1016/0022-1759(91)90198-O
A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry.
I. Nicoletti (1991)
10.1021/LA0477183
Determination of nanocrystal sizes: a comparison of TEM, SAXS, and XRD studies of highly monodisperse CoPt3 particles.
H. Borchert (2005)
10.1016/j.colsurfb.2014.11.014
Molybdenum nanoparticles-induced cytotoxicity, oxidative stress, G2/M arrest, and DNA damage in mouse skin fibroblast cells (L929).
M. A. Siddiqui (2015)
10.1016/J.PROCBIO.2012.09.025
Green biosynthesis of silver nanoparticles from Annona squamosa leaf extract and its in vitro cytotoxic effect on MCF-7 cells
Raju Vivek (2012)
10.4103/0973-1296.172968
Anticancer Activity of Chloroform Extract and Sub-fractions of Nepeta deflersiana on Human Breast and Lung Cancer Cells: An In vitro Cytotoxicity Assessment
M. Al-Oqail (2015)
Synthesis, characterization, and evaluation of antimicrobial and cytotoxic effect of silver and titanium nanoparticle
F. Martinez-Gutierrez (2010)
10.1111/j.1472-765X.2012.03219.x
Silver nanoparticles: influence of stabilizing agent and diameter on antifungal activity against Candida albicans and Candida glabrata biofilms
D. Monteiro (2012)
10.1016/j.colsurfb.2014.06.064
Synthesis, characterization and toxicological evaluation of iron oxide nanoparticles in human lung alveolar epithelial cells.
S. Dwivedi (2014)
10.1016/j.cbi.2010.12.011
Possible involvement of oxidative stress in potassium bromate-induced genotoxicity in human HepG2 cells.
Y. Zhang (2011)
10.1021/np200906s
Natural products as sources of new drugs over the 30 years from 1981 to 2010.
D. Newman (2012)
10.1016/j.colsurfb.2016.01.027
Silver nanoparticles synthesized from Adenium obesum leaf extract induced DNA damage, apoptosis and autophagy via generation of reactive oxygen species.
M. Farah (2016)
10.1021/ED084P322
Synthesis and Study of Silver Nanoparticles
S. Solomon (2007)
10.1016/0304-3835(87)90173-X
Short-term quantitative in vitro cytotoxicity assay involving an S-9 activating system.
E. Borenfreund (1987)
10.1016/0022-1759(83)90303-4
Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.
T. Mosmann (1983)
10.32388/pfg7jq
International agency for research on cancer.
J. Higginson (1974)
10.1016/j.tiv.2011.12.011
Titanium dioxide nanoparticles induced cytotoxicity, oxidative stress and DNA damage in human amnion epithelial (WISH) cells.
Q. Saquib (2012)
10.1016/j.ejmech.2010.02.054
5-Isopropylidene-3-ethyl rhodanine induce growth inhibition followed by apoptosis in leukemia cells.
S. Ravi (2010)
10.1016/j.toxlet.2008.04.015
The apoptotic effect of nanosilver is mediated by a ROS- and JNK-dependent mechanism involving the mitochondrial pathway in NIH3T3 cells.
Yi-Hong Hsin (2008)
Medicinal Plants of Saudi Arabia
J S Mossa (2000)
Studies in the flora of Arabia 2, some new and interesting species of labiatae
C. Hedge (1982)
10.1016/j.cis.2008.09.002
Silver nanoparticles: green synthesis and their antimicrobial activities.
V. Sharma (2009)
10.1016/j.tiv.2010.06.008
Protective potential of trans-resveratrol against 4-hydroxynonenal induced damage in PC12 cells.
M. A. Siddiqui (2010)
10.1016/j.toxlet.2009.07.009
PVP-coated silver nanoparticles and silver ions induce reactive oxygen species, apoptosis and necrosis in THP-1 monocytes.
Rasmus Foldbjerg (2009)
10.1016/j.ejmech.2013.07.017
Synthesis and anticancer activity of some novel 2-phenazinamine derivatives.
Xiaochun Gao (2013)
10.1088/0957-4484/14/1/321
Extracellular synthesis of silver nanoparticles by a silver-tolerant yeast strain MKY3
M. Kowshik (2003)
10.1002/CHIN.200316243
The Optical Properties of Metal Nanoparticles: The Influence of Size, Shape, and Dielectric Environment
K. L. Kelly (2003)
10.1016/j.cis.2010.02.001
Biological synthesis of metal nanoparticles by microbes.
K. Narayanan (2010)
10.1021/la103401z
Sonochemical coating of paper by microbiocidal silver nanoparticles.
R. Gottesman (2011)
10.1007/s00253-005-0179-3
The use of microorganisms for the formation of metal nanoparticles and their application
D. Mandal (2005)
10.1016/J.ARABJC.2012.04.001
Phytochemical fabrication and characterization of silver nanoparticles by using Pepper leaf broth
K. Mallikarjuna (2014)
10.1021/NL0155274
Fungus-mediated synthesis of silver nanoparticles and their immobilization in the mycelial matrix: a novel biological approach to nanoparticle synthesis
P. Mukherjee (2001)
e use of microorganisms for the formation of metal nanoparticles and their application
D. Mandal (2006)
10.1039/C0MD00069H
Silver nanoparticles—the real “silver bullet” in clinical medicine?
K. Wong (2010)
10.1007/s12645-013-0038-3
Fungus-mediated synthesis of silver nanoparticles and evaluation of antitumor activity
S. Elsonbaty (2013)
10.1016/J.FREERADBIOMED.2004.01.016
Iron oxide particles for molecular magnetic resonance imaging cause transient oxidative stress in rat macrophages.
Albrecht Stroh (2004)
10.3906/BIY-1302-21
Silver nanoparticles: cytotoxic, apoptotic, and necrotic effects on MCF-7 cells
H. Çiftçi (2013)
10.1088/0957-4484/18/10/105101
The conjugation of amyloid beta protein on the gold colloidal nanoparticles' surfaces
K. Yokoyama (2007)
10.1002/btpr.233
Biosynthesis of silver nanoparticles using Eclipta leaf
A. K. Jha (2009)
10.1039/C1GC15386B
Green synthesis of metal nanoparticles using plants
S. Iravani (2011)
10.1186/1471-2121-10-65
Anti-proliferative activity of silver nanoparticles
P. Asharani (2009)
10.1016/J.FITOTE.2003.12.012
Medicinal plant diversity in the flora of Saudi Arabia 1: a report on seven plant families.
M. A. Rahman (2004)
10.1002/EJI.1830230128
Inhibition of human immunodeficiency virus infection by the lectin jacalin and by a derived peptide showing a sequence similarity with gp120
J. Favero (1993)
10.1016/j.nano.2010.02.001
Synthesis, characterization, and evaluation of antimicrobial and cytotoxic effect of silver and titanium nanoparticles.
F. Martinez-Gutierrez (2010)



This paper is referenced by
10.1088/2043-6254/ab5103
Biofabrication of silver nanoparticles from aqueous leaf extract of Leucas aspera and their anticancer activity on human cervical cancer cells
Raviteja Chavata (2019)
10.1007/s13204-020-01585-7
Biosynthesis of silver nanoparticles by Cyperus pangorei and its potential in structural, optical and catalytic dye degradation
C. Parvathiraja (2020)
10.1016/j.jphotobiol.2020.111841
Biosynthesis of hematite nanoparticles using Rheum emodi and their antimicrobial and anticancerous effects in vitro.
D. Sharma (2020)
10.1016/j.bcab.2020.101518
Medicinal plants: Treasure trove for green synthesis of metallic nanoparticles and their biomedical applications
H. Chandra (2020)
10.1007/978-3-030-11155-7_29-1
Biosynthesized Gold and Silver Nanoparticles in Cancer Theranostics
S. K. Nethi (2020)
10.1007/s13167-020-00210-5
Genoprotective activities of plant natural substances in cancer and chemopreventive strategies in the context of 3P medicine
Lenka Koklesová (2020)
10.3390/nano10061083
Green Plasmonic Nanoparticles and Bio-Inspired Stimuli-Responsive Vesicles in Cancer Therapy Application
Valeria De Matteis (2020)
10.1155/2019/8148219
Comparative study of antitumor activity between lipophilic bismuth nanoparticles (BisBAL NPs) and chlorhexidine on human squamous cell carcinoma
Fernando Martínez-Pérez (2019)
10.1186/s43141-020-00081-3
Biogenic nanoparticles: a comprehensive perspective in synthesis, characterization, application and its challenges
S. Patil (2020)
10.1016/j.mimet.2019.105716
Myristica fragrans bio-active ester functionalized ZnO nanoparticles exhibit antibacterial and antibiofilm activities in clinical isolates.
Tijo Cherian (2019)
10.1016/j.jpba.2019.112970
Synthesis and characterization of Reishi mushroom-mediated green synthesis of silver nanoparticles for the biochemical applications.
A. Aygün (2019)
10.1155/2020/9184284
Recent Trends in Nanocarrier-Based Targeted Chemotherapy: Selective Delivery of Anticancer Drugs for Effective Lung, Colon, Cervical, and Breast Cancer Treatment
Ketao Jin (2020)
10.1038/s41598-020-73683-z
Bio fabrication of silver nanoparticles with antibacterial and cytotoxic abilities using lichens
Mona AlQahtani (2020)
10.1007/s10876-019-01697-3
Antineoplastic Biogenic Silver Nanomaterials to Combat Cervical Cancer: A Novel Approach in Cancer Therapeutics
Hamed Barabadi (2019)
10.1007/978-981-15-5179-6_8
Green Synthesis of Nanoparticles and Their Application in Cancer Therapy
V. D. Matteis (2020)
10.1007/978-981-15-0391-7
Nanoparticles and their Biomedical Applications
Ajit Kumar N. Shukla (2020)
10.1002/jemt.23604
Populus ciliata mediated synthesis of silver nanoparticles and their antibacterial activity.
M. Hafeez (2020)
10.1016/j.ceramint.2020.01.086
Osteogenic silver oxide doped mesoporous bioactive glass for controlled release of doxorubicin against bone cancer cell line (MG-63): In vitro and in vivo cytotoxicity evaluation
M. S. Rahman (2020)
10.3390/cancers12082099
Oxidative Stress and Apoptotic Responses Elicited by Nostoc-Synthesized Silver Nanoparticles against Different Cancer Cell Lines
Reham Samir Hamida (2020)
10.1016/j.colsurfb.2019.110508
Comparative safety analysis of bactericidal nano-colloids: Assessment of potential functional toxicity and radical scavenging action.
Muhammad Arslan Hashmi (2019)
10.1007/978-981-15-1702-0_2
Biological Activities of Nanoparticles and Mechanism of Action
Karan Chaudhary (2020)
10.1007/s00284-020-02058-4
Novel Biosynthesis of Copper Nanoparticles Using Zingiber and Allium sp. with Synergic Effect of Doxycycline for Anticancer and Bactericidal Activity
A. Yaqub (2020)
10.3390/nano9081089
Tangeretin-Assisted Platinum Nanoparticles Enhance the Apoptotic Properties of Doxorubicin: Combination Therapy for Osteosarcoma Treatment
S. Gurunathan (2019)
10.1016/j.carbpol.2020.116282
Seaweed polysaccharide derived bioaldehyde nanocomposite: Potential application in anticancer therapeutics.
Faisal Kholiya (2020)
10.1016/j.scp.2019.100210
Diagnosis of the multiple effect of selenium nanoparticles decorated by Asteriscus graveolens components in inhibiting HepG2 cell proliferation
Samie Yaseen Sharaf Zeebaree (2020)
10.2147/IJN.S258319
Cytotoxic and Antimicrobial Efficacy of Silver Nanoparticles Synthesized Using a Traditional Phytoproduct, Asafoetida Gum
S. Devanesan (2020)
10.1016/j.jddst.2019.101263
Biogenic silver nanoparticles using Oxalis corniculata characterization and their clinical implications
K. Karimzadeh (2019)
10.1016/j.jddst.2019.101376
Anticancer activity of silver nanoparticles synthesized using aqueous fruit shell extract of Tamarindus indica on MCF-7 human breast cancer cell line
A. Gomathi (2020)
10.1155/2020/8898360
Boswellia dalzielii-Mediated Silver Nanoparticles Inhibited Acute Myeloid Leukemia (AML) Kasumi-1 Cells by Inducing Cell Cycle Arrest
I. Adebayo (2020)
10.1155/2019/7361695
Characterization and Anticancer Potential of Silver Nanoparticles Biosynthesized from Olea chrysophylla and Lavandula dentata Leaf Extracts on HCT116 Colon Cancer Cells
Noha Moslah Al Sufyani (2019)
10.1016/j.jphotobiol.2020.111902
A nanotechnology-based new approach in the treatment of breast cancer: Biosynthesized silver nanoparticles using Cuminum cyminum L. seed extract.
Sahar Dinparvar (2020)
10.1007/978-3-030-31866-6_101
Testing Green Silver Nanoparticles for Genotoxicity, Antioxidant and Anticancer Activity
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