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Soluble Signalling Factors Derived From Differentiated Cartilage Tissue Affect Chondrogenic Differentiation Of Rat Adult Marrow Stromal Cells

Nazish Ahmed, Rita Dreier, Achim Prof. Dr. Göpferich, J. Grifka, Susanne Grässel
Published 2007 · Medicine, Biology
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Background: Chondral defects show lack of proper regeneration whereas osteochondral lesions display limited regeneration capacity. Latter is probably due to immigration of chondroprogenitor cells from the subchondral bone. Known chondroprogenitor cells for cartilage tissues are multi-potent adult marrow stromal or mesenchymal stem cells (MSCs). In vitro chondrogenic differentiation of these precursor cells usually require cues from growth and signalling factors provided in vivo by surrounding tissues and cells. We hypothesise that signalling factors secreted by differentiated cartilage tissue can initiate and maintain chondrogenic differentiation status of MSCs. Methods: To study such paracrine communication between allogenic rat articular cartilage and rat MSCs embedded in alginate beads a novel coculture system without addition of external growth factors has been established. Results: Impact of cartilage on differentiating MSCs was observed at two different time points. Firstly, sustained expression of Sox9 was observed at an early stage which indicated induction of chondrogenic differentiation. Secondly, late stage repression of collagen X indicated pre-hypertrophic arrest of differentiation. In the culture supernatant we have identified vascular endothelial growth factor alpha (VEGF-164α), matrix metalloproteinase (MMP) -13 and tissue inhibitors of MMPs (TIMP-1 and TIMP-2) which could be traced back either to the cartilage explant or to the MSCs under the influence of cartilage. Conclusion: The identified factors might be involved in regulation of collagen X gene and protein expression and therefore, may have an impact on the control and regulation of MSCs differentiation.
This paper references
10.1016/S0945-053X(03)00012-X
Tissue engineering and cell therapy of cartilage and bone.
Ranieri Cancedda (2003)
Aggrecan synthesized by mature bovine chondrocytes suspended in alginate. Identification of two distinct metabolic matrix pools.
Su San Mok (1994)
10.1016/S0945-053X(00)00094-9
Transcriptional mechanisms of chondrocyte differentiation.
Benoit de Crombrugghe (2000)
10.1002/dvdy.1212
Vascular regression is required for mesenchymal condensation and chondrogenesis in the developing limb.
Melinda Yin (2001)
10.1016/0959-437X(94)90141-O
Bone and cartilage differentiation.
A. Hari Reddi (1994)
CD 45-positive cells of haematopoietic origin enhance chondrogenic marker gene expression in rat marrow stromal cells
Nazish Ahmed
10.1083/jcb.109.5.2537
Induction and prevention of chondrocyte hypertrophy in culture
Peter Brūckner (1989)
10.1002/jcb.20652
The control of chondrogenesis.
Mary B Goldring (2006)
10.1002/1097-4636(20011205)57:3<394::AID-JBM1182>3.0.CO;2-9
Three-dimensional cartilage formation by bone marrow-derived cells seeded in polylactide/alginate amalgam.
Edward J. Caterson (2001)
10.1038/8792
Sox9 is required for cartilage formation
Weimin Bi (1999)
10.1096/fasebj.11.2.9039954
Heritable diseases of the skeleton. Part I: Molecular insights into skeletal development-transcription factors and signaling pathways.
Stefan Mundlos (1997)
10.1002/(SICI)1097-0177(199703)208:3<387::AID-AJA9>3.0.CO;2-E
Collagenase-3 (MMP-13) is expressed by hypertrophic chondrocytes, periosteal cells, and osteoblasts during human fetal bone development.
Nina Johansson (1997)
10.1016/S0092-8674(03)00551-8
TIMP-2 Mediated Inhibition of Angiogenesis An MMP-Independent Mechanism
Dong-Wan Seo (2003)
10.1083/jcb.108.1.191
Cartilage contains mixed fibrils of collagen types II, IX, and XI
Markus Mendler (1989)
10.1016/j.molmed.2005.09.002
Mesenchymal stem cell therapy to rebuild cartilage.
David Magne (2005)
10.1002/1529-0131(200105)44:5<1082::AID-ANR188>3.0.CO;2-X
The splice variants VEGF121 and VEGF189 of the angiogenic peptide vascular endothelial growth factor are expressed in osteoarthritic cartilage.
Thomas Pufe (2001)
10.1002/(SICI)1097-0177(199904)214:4<279::AID-AJA1>3.0.CO;2-W
Maturational disturbance of chondrocytes in Cbfa1-deficient mice.
Masaki Inada (1999)
10.1074/jbc.272.12.7608
The Role of the C-terminal Domain of Human Collagenase-3 (MMP-13) in the Activation of Procollagenase-3, Substrate Specificity, and Tissue Inhibitor of Metalloproteinase Interaction*
Vera Knäuper (1997)
Critical roles for collagenase-3 ( MMP 13 ) in development of growth plate cartilage and in endochondral ossification
Andrei Chagina (2005)
10.1053/joca.2001.0447
L-Sox5, Sox6 and Sox9 control essential steps of the chondrocyte differentiation pathway.
Véronique Lefebvre (2001)
10.1074/jbc.M413913200
Smad3 Induces Chondrogenesis through the Activation of SOX9 via CREB-binding Protein/p300 Recruitment*[boxs]
Takayuki Furumatsu (2005)
10.1016/S0945-053X(01)00132-9
Role of the subchondral vascular system in endochondral ossification: endothelial cell-derived proteinases derepress late cartilage differentiation in vitro.
A. V. Babarina (2001)
10.1074/jbc.271.3.1544
Biochemical Characterization of Human Collagenase-3 (*)
Vera Knäuper (1996)
The role of vascular endothelial growth factor in human dental pulp cells : induction of chemotaxis , proliferation , and differentiation and activation of the AP - 1 - dependent signaling path
K Matsushita (2000)
10.1083/jcb.200211089
Type X collagen gene regulation by Runx2 contributes directly to its hypertrophic chondrocyte–specific expression in vivo
Qiping Zheng (2003)
10.1242/dev.01461
Altered endochondral bone development in matrix metalloproteinase 13-deficient mice
Dominique Stickens (2004)
10.1002/path.1527
Vascular endothelial growth factor (VEGF) induces matrix metalloproteinase expression in immortalized chondrocytes.
Thomas Pufe (2004)
10.1016/S0925-4773(98)00210-X
Regulation of chondrocyte differentiation by Cbfa1
Ik Sun Kim (1999)
10.1002/bdrc.20048
Transcriptional control of chondrocyte fate and differentiation.
Véronique Lefebvre (2005)
10.1038/sj.onc.1202333
Both AP-1 and Cbfa1-like factors are required for the induction of interstitial collagenase by parathyroid hormone
Daniel. Porte (1999)
10.1111/j.1432-1033.1996.0576r.x
Biosynthesis and processing of type XVI collagen in human fibroblasts and smooth muscle cells.
Susanne Grässel (1996)
10.1359/jbmr.2002.17.4.639
Proteolysis involving matrix metalloproteinase 13 (collagenase-3) is required for chondrocyte differentiation that is associated with matrix mineralization.
Congzhong Wu (2002)
10.1016/j.molmed.2005.01.007
TIMP-2: an endogenous inhibitor of angiogenesis.
William G. Stetler-Stevenson (2005)
10.1006/excr.1995.1024
Terminal differentiation of chondrocytes in culture is a spontaneous process and is arrested by transforming growth factor-beta 2 and basic fibroblast growth factor in synergy.
Kurt E. Böhme (1995)
10.1136/ard.60.11.1070
Vascular endothelial growth factor in articular cartilage of healthy and osteoarthritic human knee joints
David Pfander (2001)
10.1136/gut.47.1.50
Increased expression of collagenase-3 (MMP-13) and MT1-MMP in oesophageal cancer is related to cancer aggressiveness.
Tsuyoshi Etoh (2000)
10.1002/art.1780350715
Type X collagen synthesis in human osteoarthritic cartilage. Indication of chondrocyte hypertrophy.
Klaus von der Mark (1992)
10.1002/bit.20828
Chondrogenic differentiation of bovine bone marrow mesenchymal stem cells (MSCs) in different hydrogels: influence of collagen type II extracellular matrix on MSC chondrogenesis.
Darko Bosnakovski (2006)
10.1038/9467
VEGF couples hypertrophic cartilage remodeling, ossification and angiogenesis during endochondral bone formation
Hans-Peter Gerber (1999)
10.1006/excr.2001.5278
Chondrogenic differentiation of mesenchymal stem cells from bone marrow: differentiation-dependent gene expression of matrix components.
Frank Barry (2001)
10.1007/s002239900698
Inhibition of Chondrocyte Terminal Differentiation and Matrix Calcification by Soluble Factors Released by Articular Chondrocytes
Akitoshi Jikko (1999)
10.1172/JCI118475
Cloning, expression, and type II collagenolytic activity of matrix metalloproteinase-13 from human osteoarthritic cartilage.
Pratima Mitchell (1996)
10.1042/bj3310453
Induction of matrix metalloproteinase activation cascades based on membrane-type 1 matrix metalloproteinase: associated activation of gelatinase A, gelatinase B and collagenase 3.
Susan E. Cowell (1998)
L - Sox 5 , Sox 6 and Sox 9 control essential steps of the chondro - cyte differentiation pathway
V Lefebvre (2001)
10.1074/jbc.271.29.17119
The Soluble Catalytic Domain of Membrane Type 1 Matrix Metalloproteinase Cleaves the Propeptide of Progelatinase A and Initiates Autoproteolytic Activation
H. Will (1996)
10.1006/excr.1997.3858
In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells.
Brian H. Johnstone (1998)
10.1002/1097-4652(200010)185:1<98::AID-JCP9>3.0.CO;2-1
Isolation, characterization, and chondrogenic potential of human bone marrow-derived multipotential stromal cells.
Manas K. Majumdar (2000)
10.1006/excr.1997.3849
Role of the subchondral vascular system in endochondral ossification: endothelial cells specifically derepress late differentiation in resting chondrocytes in vitro.
K Bittner (1998)
10.1056/NEJM199410063311401
Treatment of deep cartilage defects in the knee with autologous chondrocyte transplantation.
Mats Brittberg (1994)



This paper is referenced by
Modular assembly for in vitro investigation and engineering of articular cartilage
Benjamin S. Schon (2014)
10.1186/s13075-014-0453-9
Subchondral bone influences chondrogenic differentiation and collagen production of human bone marrow-derived mesenchymal stem cells and articular chondrocytes
Michaela Leyh (2014)
10.1007/978-3-319-35135-3_20
Role of PACAP and VIP Signalling in Regulation of Chondrogenesis and Osteogenesis
Tamás Juhász (2016)
10.1007/s10439-016-1575-9
Chondrocytes, Mesenchymal Stem Cells, and Their Combination in Articular Cartilage Regenerative Medicine
Arshan Nazempour (2016)
10.3109/21691401.2016.1146733
Indirect coculture of stem cells with fetal chondrons using PCL electrospun nanofiber scaffolds
Parisa Nikpou (2017)
10.1007/978-3-319-40144-7_7
Stem Cells for Articular Cartilage Repair and Regeneration
Dinesh Parate (2016)
In vitro models for cartilage engineering using primary cells and biodegradable scaffolds
Marta Alves da Silva (2011)
10.17179/excli2017-453
Effects of platelet rich plasma and chondrocyte co-culture on MSC chondrogenesis, hypertrophy and pathological responses
Rouhallah Ramezanifard (2017)
10.1016/j.peptides.2015.02.001
PACAP and VIP signaling in chondrogenesis and osteogenesis
Tamás Juhász (2015)
10.1002/term.2608
Low-oxygen conditions promote synergistic increases in chondrogenesis during co-culture of human osteoarthritic stem cells and chondrocytes.
Susan E. Critchley (2018)
10.1155/2019/5871698
Cell-to-Cell Culture Inhibits Dedifferentiation of Chondrocytes and Induces Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells
Xingfu Li (2019)
10.1038/s41598-019-51070-7
The interplay between chondrocyte spheroids and mesenchymal stem cells boosts cartilage regeneration within a 3D natural-based hydrogel
Annachiara Scalzone (2019)
MicroRNA Expression During Chondrogenic Differentiation and Inflammation of Equine Cells
Midori E Buechli (2013)
10.1016/j.biomaterials.2011.05.033
Enhanced MSC chondrogenesis following delivery of TGF-β3 from alginate microspheres within hyaluronic acid hydrogels in vitro and in vivo.
Liming Bian (2011)
PCBP 2 siRNA Reverses the Alcohol-induced Pro-fibrogenic Effects in Hepatic Stellate Cells
Ravi Shankar Shukla (2011)
10.1089/ten.TEB.2012.0273
Cell sources for articular cartilage repair strategies: shifting from monocultures to cocultures.
Jeroen Leijten (2013)
10.5966/sctm.2013-0079
Human bone marrow-derived mesenchymal stem cells display enhanced clonogenicity but impaired differentiation with hypoxic preconditioning.
Lisa B. Boyette (2014)
10.1002/stem.325
Engineering musculoskeletal tissues with human embryonic germ cell derivatives.
Shyni Varghese (2010)
10.1186/s13287-016-0301-8
Characterization of mesenchymal stem cells and fibrochondrocytes in three-dimensional co-culture: analysis of cell shape, matrix production, and mechanical performance
Mary Clare McCorry (2016)
10.1089/ten.TEA.2012.0384
Genipin-crosslinked cartilage-derived matrix as a scaffold for human adipose-derived stem cell chondrogenesis.
Nai-Chen Daniel Cheng (2013)
10.1089/ten.TEB.2012.0101
Enhancing the mesenchymal stem cell therapeutic response: cell localization and support for cartilage repair.
Sarah E Bulman (2013)
10.1016/j.jcyt.2019.10.004
Mesenchymal stem cells in the treatment of articular cartilage degeneration: new biological insights for an old-timer cell.
Alessandra Colombini (2019)
10.1016/j.peptides.2019.04.009
Decreased synovial fluid pituitary adenylate cyclase-activating polypeptide (PACAP) levels may reflect disease severity in post-traumatic knee osteoarthritis after anterior cruciate ligament injury
Bing-Yin Sun (2019)
10.1007/978-94-007-2900-1_26
Chondrogenesis from Human Mesenchymal Stem Cells: Role of Culture Conditions
Jean F. Welter (2012)
10.1002/term.235
Passaged human chondrocytes accumulate extracellular matrix when induced by bovine chondrocytes.
Nazish Ahmed (2010)
10.1007/978-1-61779-815-3
Somatic Stem Cells
Shree Ram Singh (2012)
10.1016/j.joca.2011.07.005
Structured three-dimensional co-culture of mesenchymal stem cells with chondrocytes promotes chondrogenic differentiation without hypertrophy.
Margaret E. Cooke (2011)
10.1007/978-3-319-40144-7
Bone and Cartilage Regeneration
Phuc Van Pham (2016)
Impact on cartilage repair of the knee: Patient profiling and single-stage regeneration
T. S. de Windt (2015)
10.4995/Thesis/10251/48526
Scaffold surface modifications and culture conditions as key parameters to develop cartilage and bone tissue engineering implants
Joaquín Ródenas Rochina (2015)
10.1002/jor.22047
Osteogenic and chondrogenic potential of biomembrane cells from the PMMA-segmental defect rat model.
Helen Elizabeth Gruber (2012)
10.1002/jor.21154
Interaction of periosteal explants with articular chondrocytes alters expression profile of matrix metalloproteinases.
Matthias Rickert (2010)
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