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The Effect Of Storage Time On Adipose-Derived Stem Cell Recovery From Human Lipoaspirates

Pedro P. Carvalho, Xiying Wu, Gang Yu, Isabel Rosa Dias, Manuela E Gomes, Rui Luís Reis, Jeffrey M Gimble
Published 2011 · Biology, Medicine
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Multipotent adipose-derived stromal/stem cells (ASCs) can be isolated with high yield from human subcutaneous lipoaspirates. This study reports our experience isolating, expanding, differentiating and immunophenotypically characterizing ASCs over a period of 4 days after having surgically obtained the lipoaspirate samples. The ultimate goal is to understand how to optimize the consistent isolation of ASCs from lipoaspirates. The length of time between adipose tissue harvest and processing will need to be systematically evaluated with respect to cell yield, viability, and function since some distance is likely to exist between the plastic surgeon’s office where lipoaspiration is performed and the current Good Manufacturing Practices (cGMP) laboratory where the ASCs are isolated. The objective of this study was to determine the effect of time delays on the yield and function of ASCs after collagenase digestion. We were able to isolate ASCs from lipoaspirates up to 72 h after the surgical procedure. The ASCs isolated on sequential days after the original tissue harvest proliferated, differentiated and maintained cell surface markers. We found that the initial 24-hour period is optimal for isolating ASCs with respect to cell yield and that there was no significant difference between ASC cell proliferation and differentiation ability within this period of time. In contrast, each of these parameters declined significantly for tissues maintained at room temperature for 48 or 72 h prior to isolation. These findings should be considered in the future development of standard operating procedures for cGMP processing of clinical-grade human ASCs.
This paper references
10.1053/meta.2001.21690
Thiazolidinediones and glucocorticoids synergistically induce differentiation of human adipose tissue stromal cells: biochemical, cellular, and molecular analysis.
Y D Halvorsen (2001)
10.1634/stemcells.2005-0234
Immunophenotype of human adipose-derived cells: temporal changes in stromal-associated and stem cell-associated markers.
James B. Mitchell (2006)
10.1016/j.ijom.2009.01.001
Novel maxillary reconstruction with ectopic bone formation by GMP adipose stem cells.
Karri Mesimäki (2009)
10.1080/14653240600855905
Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement.
Massimiliano Dominici (2006)
10.1016/j.jcms.2004.06.002
Autologous stem cells (adipose) and fibrin glue used to treat widespread traumatic calvarial defects: case report.
Stefan Lendeckel (2004)
10.1002/9780470571224.PSE211
Phase II Clinical Trials
Say Beng Tan (2010)
10.1080/14653240310003026
Adipose-derived adult stem cells: isolation, characterization, and differentiation potential.
Jm Gimble (2003)
10.1007/s10350-005-0052-6
A Phase I Clinical Trial of the Treatment of Crohn’s Fistula by Adipose Mesenchymal Stem Cell Transplantation
Damián García-Olmo (2005)
10.1007/s00266-007-9019-4
Cell-Assisted Lipotransfer for Cosmetic Breast Augmentation: Supportive Use of Adipose-Derived Stem/Stromal Cells
Kotaro Yoshimura (2007)
10.1007/s00384-008-0559-0
Treatment of enterocutaneous fistula in Crohn’s Disease with adipose-derived stem cells: a comparison of protocols with and without cell expansion
Damián García-Olmo (2008)
10.1091/mbc.E02-02-0105
Human adipose tissue is a source of multipotent stem cells.
Patricia A. Zuk (2002)
10.1007/DCR.0b013e3181973487
Expanded Adipose-Derived Stem Cells for the Treatment of Complex Perianal Fistula: a Phase II Clinical Trial
Damián García-Olmo (2009)
10.1089/ten.teb.2008.0423
Adipose tissue-derived stem cells and their application in bone and cartilage tissue engineering.
Tommaso Rada (2009)
10.1634/stemcells.2005-0235
The immunogenicity of human adipose-derived cells: temporal changes in vitro.
Kevin R. Mcintosh (2006)
10.1161/01.RES.0000265074.83288.09
Adipose-derived stem cells for regenerative medicine.
Jeffrey M Gimble (2007)
10.1016/j.cps.2005.08.004
Adipose tissue: stem cells and beyond.
Sunil S. Tholpady (2006)
10.1002/jcp.1138
Surface protein characterization of human adipose tissue-derived stromal cells.
Stan Gronthos (2001)
10.1111/j.1524-4741.2009.00873.x
Progenitor-enriched adipose tissue transplantation as rescue for breast implant complications.
Kotaro Yoshimura (2010)
10.1016/S1535-1513(08)79213-0
Cell-Assisted Lipotransfer for Facial Lipoatrophy: Efficacy of Clinical Use of Adipose-Derived Stem Cells
Robert L. Ruberg (2010)
10.1007/s12015-010-9147-0
Distinct Stem Cells Subpopulations Isolated from Human Adipose Tissue Exhibit Different Chondrogenic and Osteogenic Differentiation Potential
Tommaso Rada (2010)
10.1089/107632701300062859
Multilineage cells from human adipose tissue: implications for cell-based therapies.
Patricia A. Zuk (2001)
10.1080/14653240310004539
Yield of human adipose-derived adult stem cells from liposuction aspirates.
Louise B Aust (2004)
10.1517/14712598.8.9.1417
Expanded adipose-derived stem cells for the treatment of complex perianal fistula including Crohn's disease.
Damián García-Olmo (2008)
10.1097/01.prs.0000288015.70922.e4
Influences of Preservation at Various Temperatures on Liposuction Aspirates
Daisuke Matsumoto (2007)
10.1007/s00384-003-0490-3
Autologous stem cell transplantation for treatment of rectovaginal fistula in perianal Crohn's disease: a new cell-based therapy
Damián García-Olmo (2003)
10.1089/ten.2006.12.3375
Cell-assisted lipotransfer: supportive use of human adipose-derived cells for soft tissue augmentation with lipoinjection.
Daisuke Matsumoto (2006)
Extra - cellular matrix mineralization and osteoblast gene expression by human adipose tissue - derived stromal cells
D. Franklin
10.1007/978-1-60327-169-1_5
Isolation of human adipose-derived stem cells from biopsies and liposuction specimens.
Severine G. Dubois (2008)
10.1089/107632701753337681
Extracellular matrix mineralization and osteoblast gene expression by human adipose tissue-derived stromal cells.
Y D Halvorsen (2001)
The immunophenotype of human adipose derived cells : temporal changes in stromal - and stem cell - associated markers
K. McIntosh (2006)
Thia - zolidinediones and glucocorticoids synergistically induce differentiation of human adipose tissue stromal cells : biochemical , cellular , and molecular analysis
A. Bond
10.3109/14653241003649528
Yield and characterization of subcutaneous human adipose-derived stem cells by flow cytometric and adipogenic mRNA analyzes.
Gang Yu (2010)



This paper is referenced by
10.1097/PRS.0000000000004030
Effect of Cryopreservation on Human Adipose Tissue and Isolated Stromal Vascular Fraction Cells: In Vitro and In Vivo Analyses
Fabiana Cristina Zanata (2018)
10.5772/55924
Adipose Derived Stem Cells: Current State of the Art and Prospective Role in Regenerative Medicine and Tissue Engineering
Vincenzo Vindigni (2013)
10.1016/j.jcyt.2013.04.001
A non-enzymatic method for isolating human adipose tissue-derived stromal stem cells.
Forum S Shah (2013)
10.1016/j.jflm.2019.101875
Isolation and culture of human adipose-derived mesenchymal stromal/stem cells harvested from postmortem adipose tissues.
Takashi Saitō (2019)
10.1002/adhm.201500623
Exploring the Potential of Starch/Polycaprolactone Aligned Magnetic Responsive Scaffolds for Tendon Regeneration.
A I Gonçalves (2016)
The Isolation and Differentiation of Adipose Derived Mesenchymal Stem Cells
Max Merilovich (2016)
10.1177/0885328216667633
Augmenting in vitro osteogenesis of a glycine–arginine–glycine–aspartic-conjugated oxidized alginate–gelatin–biphasic calcium phosphate hydrogel composite and in vivo bone biogenesis through stem cell delivery
Nguyen Tb Linh (2016)
10.1002/jcp.26363
Crosstalk between adipose stem cells and tendon cells reveals a temporal regulation of tenogenesis by matrix deposition and remodeling.
Raquel Costa-Almeida (2018)
10.1002/jbm.a.34983
Undifferentiated human adipose-derived stromal/stem cells loaded onto wet-spun starch-polycaprolactone scaffolds enhance bone regeneration: nude mice calvarial defect in vivo study.
Pedro P. Carvalho (2014)
10.1002/term.1683
Chondrogenic potential of injectable κ-carrageenan hydrogel with encapsulated adipose stem cells for cartilage tissue-engineering applications.
Elena Geta Popa (2015)
10.1017/erm.2015.10
Critical steps in the isolation and expansion of adipose-derived stem cells for translational therapy.
Simone Elkjær Riis (2015)
Autologous Adipose Stem Cells Use for Skin Regeneration and Treatment in Humans
Mohammed Adel Salahat (2013)
10.1039/c9nr04355a
Magneto-mechanical actuation of magnetic responsive fibrous scaffolds boosts tenogenesis of human adipose stem cells.
Ana Rita Tomás (2019)
10.1371/journal.pone.0035422
Endothelial Differentiation of Human Stem Cells Seeded onto Electrospun Polyhydroxybutyrate/Polyhydroxybutyrate-Co-Hydroxyvalerate Fiber Mesh
Alessandra Arcoverde Cavalcanti Zonari (2012)
Development of magnetic responsive nanocomposite scaffolds for tendon tissue engineering
Ana Rita Tomás (2018)
10.1007/s13770-019-00238-3
Optimal Condition of Isolation from an Adipose Tissue-Derived Stromal Vascular Fraction for the Development of Automated Systems
Su Jin Lee (2020)
10.1016/J.JEVS.2014.06.023
Characterization of Nucleated Cells From Equine Adipose Tissue and Bone Marrow Aspirate Processed for Point-of-Care Use
Ivone G. Bruno (2014)
10.1088/1758-5090/ab33e8
Human platelet lysate-based nanocomposite bioink for bioprinting hierarchical fibrillar structures.
Bárbara B Mendes (2019)
10.1007/978-1-4614-5711-4_4
Characterization of MSCs: From Early Studies to the Present
Mark F. Pittenger (2013)
10.1002/9781118498026.CH12
Adipose Tissue–Derived Stem Cells and Their Regeneration Potential
Jeffrey M Gimble (2013)
10.1039/c8nr04273j
Human-based fibrillar nanocomposite hydrogels as bioinstructive matrices to tune stem cell behavior.
Bárbara B Mendes (2018)
10.1089/ten.TEC.2012.0465
Xenofree enzymatic products for the isolation of human adipose-derived stromal/stem cells.
Pedro P. Carvalho (2013)
10.1002/jcb.26573
Tendon explant cultures to study the communication between adipose stem cells and native tendon niche.
Raquel Costa-Almeida (2018)
10.1111/cpr.12493
Bi‐directional modulation of cellular interactions in an in vitro co‐culture model of tendon‐to‐bone interface
Isabel Calejo (2018)
10.1088/1748-605X/ab0de6
Exploring platelet lysate hydrogel-coated suture threads as biofunctional composite living fibers for cell delivery in tissue repair.
Raquel Costa-Almeida (2019)
10.3390/genes10060474
Immunophenotyping of a Stromal Vascular Fraction from Microfragmented Lipoaspirate Used in Osteoarthritis Cartilage Treatment and Its Lipoaspirate Counterpart
D. Polancec (2019)
10.1007/s40883-017-0029-8
Injectable Hyaluronic Acid Hydrogels Enriched with Platelet Lysate as a Cryostable Off-the-Shelf System for Cell-Based Therapies
Lisete S. Neves (2017)
10.3892/ijmm.2018.3979
In vitro enhancement and functional characterization of neurite outgrowth by undifferentiated adipose-derived stem cells.
Vesna Bucan (2019)
10.1016/j.actbio.2017.09.014
Tissue-engineered magnetic cell sheet patches for advanced strategies in tendon regeneration.
A I Gonçalves (2017)
10.1016/j.nano.2018.02.008
Triggering the activation of Activin A type II receptor in human adipose stem cells towards tenogenic commitment using mechanomagnetic stimulation.
A I Gonçalves (2018)
10.1002/stem.629
Concise review: Adipose-derived stromal vascular fraction cells and stem cells: let's not get lost in translation.
Jeffrey M. Gimble (2011)
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