Online citations, reference lists, and bibliographies.
← Back to Search

α-Tocopherol Enrichment Of Monocytes Decreases Agonist-Induced Adhesion To Human Endothelial Cells

K. Islam, S. Devaraj, I. Jialal
Published 1998 · Biology

Cite This
Download PDF
Analyze on Scholarcy
Background —Monocyte-endothelium adhesion is a crucial early event in atherogenesis. Several reports indicate that α-tocopherol (AT) is a potent antioxidant in plasma and LDL and also has intracellular effects that are antiatherogenic. Recently, it has been shown that AT supplementation results in decreased monocyte–endothelial cell adhesion. However, there is a paucity of data on the mechanisms by which AT inhibits adhesion of monocytes. We studied the effect of AT enrichment of a human monocytic cell line, U937, on adhesion to human umbilical vein endothelial cells (HUVECs). Methods and Results —Both lipopolysaccharide (LPS)– and N -formyl-methionyl-leucyl-phenylalanine (FMLP)–stimulated U937 adhesion to HUVECs were studied. AT (50 and 100 μmol/L) significantly decreased adhesion of both LPS- and FMLP-stimulated U937 cells to HUVECs (LPS-treated cells, P <0.0125; FMLP-treated cells, P <0.05). Expression of the adhesion molecules CD11a, CD11b, CD11c, very late antigen-4 (VLA-4), and L-selectin, as assessed by flow cytometry, was increased in the stimulated U937 cells, and AT resulted in significant reduction in the expression of CD11b and VLA-4. In addition, activation of the transcription factor nuclear factor-κB (NF-κB), as assessed by gel shift assays, was inhibited by pretreatment with AT in LPS-treated U937 cells. Conclusions —AT significantly decreases adhesion of activated monocytes to endothelial cells by decreasing expression of CD11b and VLA-4 on monocytes, possibly by inhibiting the activation of NF-κB.
This paper references
Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
M. Fried (1981)
Isolation of a rel-related human cDNA that potentially encodes the 65-kD subunit of NF-kappa B.
S. Ruben (1991)
Antioxidants inhibit the expression of intercellular cell adhesion molecule-1 and vascular cell adhesion molecule-1 induced by oxidized LDL on human umbilical vein endothelial cells.
L. Cominacini (1997)
Perturbation of cultured human endothelial cells by atherogenic levels of low density lipoprotein.
J. Holland (1988)
The inducible transcription activator NF-κB: regulation by distinct protein subunits
P. Baeuerle (1991)
The effects of alpha tocopherol supplementation on monocyte function. Decreased lipid oxidation, interleukin 1 beta secretion, and monocyte adhesion to endothelium.
S. Devaraj (1996)
Native LDL increases endothelial cell adhesiveness by inducing intercellular adhesion molecule-1.
D. M. Smalley (1996)
Inhibition of HIV‐1 replication and NF-x B activity by cysteine and cysteine derivatives
S. Mihm (1991)
Endothelial-leukocyte adhesion molecules in human disease.
M. Bevilacqua (1994)
Comparison of supplementation of RRR-alpha-tocopherol and racemic alpha-tocopherol in humans. Effects on lipid levels and lipoprotein susceptibility to oxidation.
P. Reaven (1993)
Protective role of vitamin E in biological systems.
L. Packer (1991)
Modified low density lipoprotein and its constituents augment cytokine-activated vascular cell adhesion molecule-1 gene expression in human vascular endothelial cells.
B. Khan (1995)
A 65-kappaD subunit of active NF-kappaB is required for inhibition of NF-kappaB by I kappaB.
P. Baeuerle (1989)
Atherogenic concentrations of low-density lipoprotein enhance endothelial cell generation of epoxyeicosatrienoic acid products.
K. Pritchard (1990)
Endothelial nuclear factor-kappa B and the initiation of the atherosclerotic lesion.
T. Collins (1993)
Oxidized lipoprotein (a) induces cell adhesion molecule Mac-1 (CD 11b) and enhances adhesion of the monocytic cell line U937 to cultured endothelial cells.
M. Ragab (1996)
Monocytes from patients with combined hypercholesterolemia-hypertriglyceridemia and isolated hypercholesterolemia show an increased adhesion to endothelial cells in vitro: II. Influence of intrinsic and extrinsic factors on monocyte binding.
J. Jongkind (1995)
Enhancement of monocyte adhesion to endothelial cells by oxidatively modified low-density lipoprotein is mediated by activation of CD11b.
C. Weber (1995)
Activation of NFκB by the respiratory burst of macrophages
N. Kaul (1996)
Inhibition of NF-kappa B DNA binding activity by alpha-tocopheryl succinate.
Y. Suzuki (1993)
Inhibition of NF-κB Activation by Vitamin E Derivatives
Y. Suzuki (1993)
An atherogenic diet rapidly induces VCAM-1, a cytokine-regulatable mononuclear leukocyte adhesion molecule, in rabbit aortic endothelium.
H. Li (1993)
Effect of dietary supplementation with alpha-tocopherol on the oxidative modification of low density lipoprotein.
I. Jialal (1992)
A gel electrophoresis method for quantifying the binding of proteins to specific DNA regions: application to components of the Escherichia coli lactose operon regulatory system
M. M. Garner (1981)
I kappa B: a specific inhibitor of the NF-kappa B transcription factor.
P. Baeuerle (1988)
Leucocyte-endothelial interactions and regulation of leucocyte migration
D. Adams (1994)
Activated transcription factor nuclear factor-kappa B is present in the atherosclerotic lesion.
K. Brand (1996)
Alpha-tocopherol inhibits agonist-induced monocytic cell adhesion to cultured human endothelial cells.
R. Faruqi (1994)
Involvement of the immune system in human atherogenesis: current knowledge and unanswered questions.
P. Libby (1991)
Vascular cell adhesion molecule-1 (VCAM-1) gene transcription and expression are regulated through an antioxidant-sensitive mechanism in human vascular endothelial cells.
N. Marui (1993)
Reactive oxygen intermediates as apparently widely used messengers in the activation of the NF‐kappa B transcription factor and HIV‐1.
R. Schreck (1991)
Vitamin E: beyond antioxidant function.
M. Traber (1995)
Intracellular thiols regulate activation of nuclear factor kappa B and transcription of human immunodeficiency virus.
F. Staal (1990)
Monocyte/macrophage recruitment and expression of endothelial adhesion proteins in human atherosclerotic lesions.
C. Duplàa (1996)
Levels of Soluble Cell Adhesion Molecules in Patients With Dyslipidemia
Anne M. Hackman (1996)
Vitamin E inhibits low-density lipoprotein-induced adhesion of monocytes to human aortic endothelial cells in vitro.
A. Martín (1997)
Antisense oligonucleotides to the p65 subunit of NF-kappa B block CD11b expression and alter adhesion properties of differentiated HL-60 granulocytes
J. A. Sokoloski (1993)
Neovascular expression of E-selectin, intercellular adhesion molecule-1, and vascular cell adhesion molecule-1 in human atherosclerosis and their relation to intimal leukocyte content.
K. O'brien (1996)
The effects of alpha-tocopherol on critical cells in atherogenesis.
S. Devaraj (1998)
Increased adhesiveness of isolated monocytes to endothelium is prevented by vitamin C intake in smokers.
C. Weber (1996)
The pathogenesis of atherosclerosis: a perspective for the 1990s
R. Ross (1993)
Activation of DNA-binding activity in an apparently cytoplasmic precursor of the NF-κB transcription factor
P. Baeuerle (1988)
Linoleic acid activates nuclear transcription factor-kappa B (NF-kappa B) and induces NF-kappa B-dependent transcription in cultured endothelial cells.
B. Hennig (1996)

This paper is referenced by
Oxidative stress and antioxidant therapy: their impact in diabetes-associated erectile dysfunction.
L. D. De Young (2004)
Role of Oxidized Lipids in Atherosclerosis
M. Garelnabi (2012)
α-Tocopherol decreases CD36 expression in human monocyte-derived macrophages
S. Devaraj (2001)
The effects of α-tocopherol on oxidative damage and serum levels of Clara cell protein 16 in aspiration pneumonitis induced by bile acids
H. Alaçam (2013)
Differential inhibition by α- and β-tocopherol of human erythroleukemia cell adhesion: role of integrins
I. Breyer (2001)
Emerging Role of Flavonoids in Inhibition of NF-B-Mediated Signaling Pathway : A Review
K. Devi (2009)
Regulation of gene expression by α-tocopherol
A. Azzi (2004)
Effect of PDE5 inhibition combined with free oxygen radical scavenger therapy on erectile function in a diabetic animal model
L. D. Young (2003)
Inhibitory effect of delta-tocotrienol, a HMG CoA reductase inhibitor, on monocyte-endothelial cell adhesion.
Jun-Tzu Chao (2002)
Role of Oxidants and Antioxidants in Atherosclerosis: Results of In Vitro and In Vivo Investigations
R. Siekmeier (2007)
Symposium: Molecular mechanisms of protective effects of vitamin E in atherosclerosis
R. Eckhardt (2001)
Enteral feeding enriched with carotenoids normalizes the carotenoid status and reduces oxidative stress in long-term enterally fed patients.
N. Vaisman (2006)
Inflammation and Cancer
A. Hossain (2007)
Nutritional modulation of vascular function
Michela Zanetti (2012)
Vitamin E attenuates myocardial ischemia-reperfusion injury in murine AIDS
Y. Chen (2007)
The role of α-tocopherol in preventing disease
A. Azzi (2004)
Diverse agents act at multiple levels to inhibit the Rel/NF-κB signal transduction pathway
J. Epinat (1999)
Foreign body-type multinucleated giant cell formation is potently induced by alpha-tocopherol and prevented by the diacylglycerol kinase inhibitor R59022.
A. K. McNally (2003)
Antioxidants and endothelium protection.
D. Praticò (2005)
Tocotrienol is the most effective vitamin E for reducing endothelial expression of adhesion molecules and adhesion to monocytes.
A. Theriault (2002)
The Efficacy of α-lipoic Acid on the Endotoxin-induced Acute Lung Injury
J. Huh (2007)
A standardized bamboo leaf extract inhibits monocyte adhesion to endothelial cells by modulating vascular cell adhesion protein-1
S. Choi (2013)
Cardiovascular disease: C-reactive protein and the inflammatory disease paradigm: HMG-CoA reductase inhibitors, alpha-tocopherol, red yeast rice, and olive oil polyphenols. A review of the literature.
L. Patrick (2001)
Oxidized LDL Upregulates Angiotensin II Type 1 Receptor Expression in Cultured Human Coronary Artery Endothelial Cells: The Potential Role of Transcription Factor NF-&kgr;B
D. Li (2000)
Heart disease and single-vitamin supplementation.
M. Traber (2007)
Toll-like receptors 2 and 4 mediate hyperglycemia induced macrovascular aortic endothelial cell inflammation and perturbation of the endothelial glycocalyx.
R. Pahwa (2016)
Inflammation and infection in acute coronary syndrome.
N. Albert (2000)
Low-Density Lipoprotein Postsecretory Modification, Monocyte Function, and Circulating Adhesion Molecules in Type 2 Diabetic Patients With and Without Macrovascular Complications The Effect of α-Tocopherol Supplementation
S. Devaraj (2000)
Vascular Protective Role of Vitexicarpin Isolated from Vitex rotundifolia in Human Umbilical Vein Endothelial Cells
S. M. Lee (2011)
Anti-inflammatory effects of different drugs/agents with antioxidant property on endothelial expression of adhesion molecules.
Yung-Hsiang Chen (2006)
Inhibition of cytokine-induced IkappaB kinase activation as a mechanism contributing to the anti-atherogenic activity of tilianin in hyperlipidemic mice.
Kung-woo Nam (2005)
Vitamin E and heart disease: basic science to clinical intervention trials.
W. A. Pryor (2000)
See more
Semantic Scholar Logo Some data provided by SemanticScholar